A5101417733- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Cancer Genomics and Diagnostics
- Intraocular Surgery and Lenses
- Retinal Imaging and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Ophthalmology and Visual Impairment Studies
- Nanoplatforms for cancer theranostics
- Retinal and Macular Surgery
- Metabolism, Diabetes, and Cancer
- Connexins and lens biology
- Cancer Research and Treatments
- Melanoma and MAPK Pathways
- Circadian rhythm and melatonin
- Urinary Bladder and Prostate Research
- Inflammatory Biomarkers in Disease Prognosis
- Lung Cancer Treatments and Mutations
- Urinary Tract Infections Management
- Bacterial Genetics and Biotechnology
- Cancer Cells and Metastasis
- Viral-associated cancers and disorders
Nanjing Drum Tower Hospital
2016-2025
Affiliated Hospital of Nantong University
2013-2025
Nantong University
2013-2025
Model Animal Research Center
2020-2024
Nanjing Medical University
2017-2024
Soochow University
2024
Nanjing University
2018-2023
Nanjing Jiangning Hospital
2023
National Yang Ming Chiao Tung University
2020-2021
Data Harbor (United States)
2015
Abstract Strategies that enhance the function of T cells are critical for immunotherapy. One negative regulator T-cell activity is ligand PD-L1, which expressed on dentritic (DCs) or some tumor and functions through binding programmed death-1 (PD-1) receptor activated cells. Here we described first time a non-viral mediated approach to reprogram primary human by disruption PD-1. We showed gene knockout PD-1 electroporation plasmids encoding sgRNA Cas9 was technically feasible. The inhibitory...
BACKGROUND. Recent genomic and bioinformatic technological advances have made it possible to dissect the immune response personalized neoantigens encoded by tumor-specific mutations. However, timely efficient identification of is still a major obstacle neoantigen-based cancer immunotherapy.
Human cytotoxic T-lymphocyte membrane-camouflaged nanoparticles combined with low-dose irradiation: a new approach to enhance drug targeting in gastric cancer Lianru Zhang, Rutian Li, Hong Chen, Jia Wei, Hanqing Qian, Shu Su, Jie Shao, Lifeng Wang, Xiaoping Baorui Liu The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School Nanjing University and Clinical Institute University, Nanjing, People’s Republic of China Abstract: Cell membrane-derived are becoming more...
Abstract Poor infiltration of activated lymphocytes into tumors represents a fundamental factor limiting the therapeutic effect adoptive cell immunotherapy. A tumor-penetrating peptide, iRGD, has been widely used to deliver drugs tumor tissues. In this study, we demonstrate for first time that iRGD could also facilitate in both 3D spheroids and several xenograft mouse models. addition, combining modification with PD-1 knockout reveals superior anti-tumor efficiency. Mechanistic studies...
The successful use of immune cell checkpoint inhibitors PD-1 and PD-L1, over the past 5 y has raised concern using immunotherapy to treat various cancers. Epstein-Barr virus-associated gastric cancer (EBVaGC) exhibits high infiltration lymphocytes amplification immune-related genes including PD-L1 as distinguished from virus-non-associated (EBVnGC). Here, we presume that this PD-1/PD-L1 pathway may hinder efficacy adoptive T therapy toward EBVaGC. These studies reveal possibility generating...
Abstract Diabetic cardiomyopathy is a progressive disease in diabetic patients, and myocardial insulin resistance contributes to its pathogenesis through incompletely-defined mechanisms. Striated muscle preferentially expressed protein kinase (SPEG) has two kinase-domains critical cardiac regulator. Here we show that SPEG phosphorylated on Ser 2461 /Ser 2462 /Thr 2463 by B (PKB) response insulin. PKB-mediated phosphorylation of activates second kinase-domain, which turn phosphorylates...
Abstract Background The introduction of the anti-PD-1 antibody has greatly improved clinical outcomes patients with non-small cell lung cancer (NSCLC). In this study, we retrospectively analyzed efficacy PD-1 antibody-based therapy in locally advanced inoperable or metastatic NSCLC and reported an association between peripheral blood biomarkers response these patients. Methods This single-center study included medical record data treated as a first-line subsequent line treatment, either...
<title>Abstract</title> Malignant pleural mesothelioma is an aggressive and lethal malignancy that arises from the mesothelial cells lining pleura. Diagnosis commonly occurs at advanced stage, rendering patients unsuitable for surgical intervention. Pharmacological interventions have shown limited success in controlling disease. In present study, we a case of malignant with large effusion, demonstrating elevated expression PD-L1 (TPS 90%) focal amplification <italic>MET</italic> gene...
<title>Abstract</title> <bold>Background:</bold> The use of machine learning (ML) techniques, particularly XGBoost and logistic regression, to predict sarcopenia among postsurgical gastric cancer patients has gained significant attention in recent research. Sarcopenia, characterized by the progressive loss skeletal muscle mass strength, is a serious concern these due its association with poor postoperative outcomes, including increased morbidity mortality. In this study, was used establish...
3046 Background: Recent genomic and bioinformatic technological advances have made it possible to dissect the immune response personalized neoantigens encoded by tumor-specific mutations.However, rapid efficient identification of is still fraught with difficulty, a systematic evaluation based immunotherapy in advanced refractory epithelial tumors lacking. Methods: Tumor ctDNA samples from 16 cancer patients were underwent mutational profiling cancer-associated genes panel. Neoantigens...
Age-related cataract (ARC) is one of the most common causes severe visual impairment among elderly worldwide with four subtypes, such as cortical, nuclear, subcapsular, and mixed types. DNA damage malfunction repair are believed to contribute pathogenesis ARC. This study examined associations 18 single nucleotide polymorphisms (SNPs) in genes (BLM, WRN, ERCC6, OGG1) ARC Han Chinese from Jiangsu Eye Study, a population-based epidemiologic study. We also determined possible functional...
Abstract The incidence of melanoma is rising globally including China. Comparing to Caucasians, the non‐cutaneous melanomas significantly higher in Chinese. Herein, we performed genomic profiling 89 Chinese surgically resected primary melanomas, acral ( n = 54), cutaneous 22), and mucosal 13), by hybrid capture‐based next‐generation sequencing. We show that tended harbor more pathogenic mutations than other types melanoma, though biological significance this finding remains uncertain....
Insulin is a potent inducer of mRNA transcription and translation, contributing to metabolic regulation. has also been suggested regulate stability through the processing body (P-body) molecular machinery. However, whether how insulin regulates via P-bodies not clear. Here we show that E3-ligase TRIM24 critical factor linking signalling P-bodies. Upon stimulation, protein kinase B (PKB, known as Akt) phosphorylates stimulates its shuttling from nucleus into cytoplasm. interacts with several...
To assess the association of plasma cytokines and growth factor levels with clinical characteristics inflammatory indices in patients gastric cancer. Plasma samples derived from 99 cancer were used for analysis. Levels interferon (IFN)-γ, tumor (TGF)-β1, necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p40, IL-12p70, vascular endothelial (VEGF) measured by Luminex suspension array technology. The between cytokine/growth demographic/clinical was assessed....
Adoptive cellular immunotherapy (ACI) has been demonstrated to be a promising cancer therapeutic; however, the inefficient migration of adoptive immune cells tumors is one rate-limiting factors ACI. The present study investigated whether 2 Gy low dose irradiation (LDI) was able increase lymphocytes gastric cells. Treatment with LDI resulted in marked chemokine (C-X-C motif) ligand 9 (CXCL9) and CXCL10 production from cell lines. A Transwell chamber assay enhanced transmigration cytotoxic T...
To determine the incidence of anterior chamber (AC) instability during phacoemulsification (phaco) combined with intraocular lens implantation and investigate its effect on tissues. Among 248 enrolled eyes, 121 127 eyes were categorized into irrigation nonirrigation groups, respectively, depending use a self-made maintainer (ACM) phaco. AC stability was evaluated using operating microscopy intraoperative optical coherence tomography (iOCT). Slit-lamp examination flare cells performed 1 day...
Abstract Sarcoplasmic/endoplasmic reticulum calcium ATPase SERCA2 mediates re-uptake from the cytosol into sarcoplasmic reticulum, and its dysfunction is a hallmark of heart failure. Multiple factors have been identified to modulate activity, however, regulation still not fully understood. Here we identify Ral-GTPase activating protein RalGAPα1 as critical regulator in cardiomyocytes through downstream target RalA. induced by pressure overload, deficiency causes cardiac exacerbates...