A5008476664- Bacterial Genetics and Biotechnology
- S100 Proteins and Annexins
- Chronic Myeloid Leukemia Treatments
- Peptidase Inhibition and Analysis
- Probiotics and Fermented Foods
- Redox biology and oxidative stress
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Lipid Membrane Structure and Behavior
- Cancer Research and Treatments
- Vibrio bacteria research studies
- Retinoids in leukemia and cellular processes
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Heat shock proteins research
- Chronic Lymphocytic Leukemia Research
- Mitochondrial Function and Pathology
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Eosinophilic Disorders and Syndromes
- Sepsis Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Genomics and Phylogenetic Studies
- Caveolin-1 and cellular processes
- Cancer, Lipids, and Metabolism
- Cell death mechanisms and regulation
Institute of Haematology and Blood Transfusion
2007-2020
Charles University
2000-2012
After reading many 2-DE-based articles featuring lists of the differentially expressed proteins, one starts experiencing a disturbing déjà vu. The same proteins seem to predominate regardless experiment, tissue or species. To quantify occurrence individual in 2-DE experiment reports, we compiled identities identified human, mouse, and rat tissues published three recent volumes Proteomics calculated appearance most predominant dataset. frequently protein is highly abundant glycolytic enzyme...
To find out membrane tolerance strategy to ethanol in Bacillus subtilis that possesses a powerful system of protection against environmental stresses.Cytoplasmic membranes B. were severely affected by even short-term exposure 3% (v/v) ethanol: the growth rate and protein synthesis markedly reduced, no adaptive alterations phospholipids detected. Simultaneously, steady-state DPH fluorescence anisotropy (r(ss)) showed rigidity increased substantially. Analysis phosphoproteome using vitro...
Chronic myeloid leukemia (CML) is a malignant hematopoietic disorder distinguished by the presence of BCR‑ABL1 fused oncogene with constitutive kinase activity. Targeted CML therapy specific tyrosine inhibitors (TKIs) leads to marked improvement in survival patients and their quality life. However, development resistance TKIs remains critical issue for subset patients. The most common cause are numerous point mutations gene, followed less multiple mutation-independent mechanisms. Recently,...
Chronic myeloid leukemia (CML) therapy has markedly improved patient prognosis after introduction of imatinib mesylate for clinical use. However, a subset patients develops resistance to and other tyrosine kinase inhibitors (TKIs), mainly due point mutations in the region encoding domain fused BCR-ABL oncogene. To identify potential therapeutic targets imatinib‑resistant CML cells, we derived imatinib-resistant CML-T1 human cell line clone (CML-T1/IR) by prolonged exposure growth media....
The resistance of malignant cells to chemotherapy calls for the development novel anti-cancer drugs. TNF-related apoptosis-inducing ligand (TRAIL) is a pro-apoptotic cytokine, which selectively induces apoptosis in cells. We derived two TRAIL-resistant HL-60 subclones, HL-60/P1 and HL-60/P2, from TRAIL-sensitive acute promyelocytic leukemia cell line. To identify therapeutically exploitable "weaknesses" that could be used as molecular targets their elimination, we performed proteomic (2-DE)...