A5000225403- Aluminum Alloy Microstructure Properties
- Aluminum Alloys Composites Properties
- Lipid Membrane Structure and Behavior
- Bacterial Genetics and Biotechnology
- Material Properties and Applications
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Metallurgy and Material Science
- Advanced machining processes and optimization
- Advanced Surface Polishing Techniques
- Metal Alloys Wear and Properties
- Materials Engineering and Processing
- Corrosion Behavior and Inhibition
- Metal and Thin Film Mechanics
- Animal Virus Infections Studies
- Erosion and Abrasive Machining
- Photosynthetic Processes and Mechanisms
- Diagnosis and Treatment of Venous Diseases
- Peripheral Artery Disease Management
- Manufacturing Process and Optimization
- Protein Structure and Dynamics
- Metallurgical and Alloy Processes
- Venous Thromboembolism Diagnosis and Management
- Tribology and Wear Analysis
- Photoreceptor and optogenetics research
Jan Evangelista Purkyně University in Ústí nad Labem
2015-2024
University of South Bohemia in České Budějovice
2020
Czech Hydrometeorological Institute
2020
Research Institute of Inorganic Chemistry
2014-2018
Charles University
1995-2016
University of Regensburg
2006-2016
Institute of Technology and Business
2013
Max Planck Society
2010
General University Hospital in Prague
2005-2006
St. Charles Hospital
2006
Abstract Surfactin, a highly powerful biosurfactant produced by various strains of the genus Bacillus, exhibits antibacterial, antiviral, antitumor and hemolytic action. This anionic cyclic lipopeptide is constituted heptapeptide interlinked with β-hydroxy fatty acid. Due to its amhipathic nature surfactin incorporates into phospholipid bilayer induces permeabilization perturbation target cells. The rising antibiotic resistance as well number remarkable activities shows that it deserves...
The environment in which managers currently operate is dynamic and turbulent. This fact affects the great complexity dynamism of changes. Thus, decision-making becomes more complex context sustainability. Decisive becoming rare, challenge globalization increasing. Under its influence, no longer deterministic. Decision-making processes prevail conditions uncertainty. In this context, there a very intense need to be acquainted with progressive tools support management apply acquired knowledge...
To find out membrane tolerance strategy to ethanol in Bacillus subtilis that possesses a powerful system of protection against environmental stresses.Cytoplasmic membranes B. were severely affected by even short-term exposure 3% (v/v) ethanol: the growth rate and protein synthesis markedly reduced, no adaptive alterations phospholipids detected. Simultaneously, steady-state DPH fluorescence anisotropy (r(ss)) showed rigidity increased substantially. Analysis phosphoproteome using vitro...
Derivatization of biologically active peptides by conjugation with fluorophores or radionuclide-bearing moieties is an effective and commonly used approach to prepare molecular tools diagnostic agents. Whereas lysine, cysteine, N-terminal amino acids have been mostly for peptide conjugation, we describe a new, widely applicable based on the nonclassical bioisosteric replacement guanidine group in arginine functionalized carbamoylguanidine moiety. Four arginine-containing receptor ligands...
This article deals with the analysis of subsurface deformation effects materials due to periodic action liquid droplets, each a constant volume approximately 36 mm3 distributed spatial frequency 20,000 i/mm. Sample grooves were analyzed within standoff distances where prevailing mechanism is acceleration culmination depletion using stair trajectory avoid possible Doppler effect. Using X-ray μ-CT, network cavities corresponding fractal shape was identified below surface. It apparent that...
Production and control of accurate surface bring some issues. There are technological limits machining, but we have to know how these machined surfaces due functional life-time properties, which affected mainly by roughness surface. Most important part system measuring is suppression nominal shape scanned profile, its filtration according standard ISO 4288 evaluation the relevant standards. This article described process profile. If operator instrument omits aspects at this stage, obtain...
Peening techniques are nowadays attracting more research attention due to their association with the extending of service life and improving surface texture engineering components. Ultrasonic pulsating water jet peening represents a new way mechanical treatment. Accelerated droplets via hammer effect cause small elastic-plastic deformations on surface. This work deals aluminum alloy using an ultrasonic jet, where periodically acting were used as medium. The aim was feasibility study process...
In optically discontinuous and/or anisotropic environments such as lipid membranes, the radiative decay rate is dependent on orientation of emission dipole. A theory fluorescence and depolarization in membranes has been developed that takes orientational dependence into account. The predicts nonexponential total intensity anisotropy different than one isotropic systems. 1,6-diphenyl-1,3,5-hexatriene L-α-dipalmitoyl-phosphatidylcholine large unilamellar vesicles analyzed terms this theory. It...
The article is dedicated to research on the elimination of high iron content (above 3-4%) in aluminium alloys through sedimentation. aim was determine effect sedimentation time reducing material from a refining bath with and identify phases formed structure. Melts were prepared obtained bath, which consisted an AlSi12 alloy 3-4% Fe content. After melting, carried out for 2 hours, 4 6 hours. Sedimentation conducted while maintaining liquid state throughout entire period. cooling castings,...
The most important aim of chemical pre-treatment is the removal contaminants, corrosion products etc. from material surface and obtaining such a basic surface, which will provide sufficient resistance base suitable conditions for adhesion paints or other finish coatings to steel material. When selecting method it should be considered whether degree preparation provides required level cleanliness also roughness profile coating applied after pre-treatment. paper focuses on SEM EDS analysis...
The aim of the research was to compare a classical (turning) machining and an abrasive-free ultrasonic (bufo) at three different materials. surface evaluated on basis Olympus LEXT 3100 measuring roughness hardness HV5. An set I - 4 consisted generator, power output 630 W working frequency 22 kHz ± 10%, used for research. Main results are: increasing HV5 machined surface, lowering parameters Ra application machining, Rz not statistically proved machining. It is possible say according visual...
Abstract An on‐line microfluidic system for determination of dissociation constants enzyme/substrate weak interactions by nanoESI‐MS is introduced. The microchip was designed to permit the enzyme and substrate mix molecular diffusion in a pressure‐driven laminar flow. Introduction reagent solutions into chip an optimized combination micropump autosampler enable automation measurements. performance tested monitoring non‐covalent hen egg white lysozyme with N ‐acetyl glucosamine oligomers....
The paper deals with research focused on the use of fillers in field polymeric materials produced by additive technology SLA (stereolithography). aim is to evaluate 3D printing parameters, mechanical properties (tensile strength, hardness), and interaction individual phases (polymer matrix filler) composite using SEM analysis. tested were cotton flakes ground carbon fibres different proportions. For photosensitive resins, as filler was found have a positive effect not only under static but...