A5074170934- Nanoparticles: synthesis and applications
- Lipid Membrane Structure and Behavior
- Advanced Electron Microscopy Techniques and Applications
- Antimicrobial Peptides and Activities
- Integrated Circuits and Semiconductor Failure Analysis
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Graphene and Nanomaterials Applications
- Plant Stress Responses and Tolerance
- Vibrio bacteria research studies
- Air Quality and Health Impacts
- Metabolomics and Mass Spectrometry Studies
- Bacterial Genetics and Biotechnology
- Pneumonia and Respiratory Infections
- Diamond and Carbon-based Materials Research
- Microplastics and Plastic Pollution
- Arsenic contamination and mitigation
- Electron and X-Ray Spectroscopy Techniques
- Force Microscopy Techniques and Applications
- Ion-surface interactions and analysis
- Selenium in Biological Systems
- Advanced X-ray Imaging Techniques
- Probiotics and Fermented Foods
- Heavy Metal Exposure and Toxicity
- Aluminum toxicity and tolerance in plants and animals
Czech Academy of Sciences, Institute of Molecular Genetics
2018-2025
Czech Academy of Sciences
2018-2022
Charles University
2016-2021
In the body, engineered nanoparticles (NPs) may be recognized and processed by immune cells, among which macrophages play a crucial role. We evaluated effects of selected NPs [NM-100 (TiO2), NM-110 (ZnO), NM-200 (SiO2), NM-300 K (Ag)] on THP-1 macrophage-like cells. The cells were exposed to subcytotoxic concentrations (1–25 µg/mL) expression immunologically relevant genes (VCAM1, TNFA, CXCL8, ICAM1, CD86, CD192, IL1B) was analyzed RT-qPCR. cytokines, growth factors surface molecules...
Arsenic (As) contaminates the food chain and decreases agricultural production through impairing plants, particularly due to oxidative stress. To better understand As tolerance mechanisms, two contrasting tobacco genotypes: As-sensitive
Rapid progress in the development of highly efficient nanoparticle-based construction technologies has not always been accompanied by a corresponding understanding their effects on human health and ecosystems. In this study, we compare toxicological pristine TiO2, ZnO, SiO2, coated SiO2 nanoparticles, evaluate suitability as additives to consolidants weathered materials. First, water soluble tetrazolium 1 (WST-1) lactate dehydrogenase (LDH) assays were used determine viability alveolar A549...
Biocompatible DNA origami nanostructures (DONs) attract significant interest as potential targeted drug carriers. Their sensitivity to various environmental triggers is beneficial a release or decomposition mechanism, but it also hinders their stability; therefore, they require some extent of cross-linking. Using cisplatin, we demonstrate that molecule can both act therapeutic and cross-linking agent for DONs. In this work, triangular two-dimensional DONs are loaded with ∼1000 cisplatin...
Abstract Tens of thousands rapidly evolving long non-coding RNA (lncRNA) genes have been identified, but functions were assigned to relatively few them. The lncRNA contribution the mouse oocyte physiology remains unknown. We report evolutionary history and functional analysis Sirena1, most expressed 10th abundant poly(A) transcript in oocytes. Sirena1 appeared common ancestor rat became engaged two different post-transcriptional regulations. First, antisense oriented Elob pseudogene...
Bovine serum albumin-embedded Au nanoclusters (BSA-AuNCs) are thoroughly probed by continuous wave electron paramagnetic resonance (CW-EPR), light-induced EPR (LEPR), and sequences of microscopic investigations performed via high-resolution transmission microscopy (HR-TEM), scanning (STEM), energy dispersive X-ray analysis (EDS). To the best our knowledge, this is first report analyzing BSA-AuNCs CW-EPR/LEPR technique. Besides presence Au(0) Au(I) oxidation states in BSA-AuNCs, authors...
Cryo-electron tomography (cryo-ET) has transformed structural biology, offering unparalleled insights into biological samples at near-native state [1]. However, its efficacy relies on thin specimens, typically below 200 nm, achievable through Cryogenic Focused Ion Beam (cryo-FIB) milling [2]. Yet, accurately targeting regions of interest (ROIs) during this process remains a challenge. Fluorescence labeling coupled with fluorescence light microscopy (FLM) enhances precision [3], but...
The originality of the present study is description CuO NP interaction with earthworm immune cells and subsequent physiological response from in vitro perspective.
DNA origami nanostructures are emerging as a bottom-up nanopatterning approach. Direct combination of this approach with top-down nanotechnology, such ion beams, has not been considered because the soft nature material. Here we demonstrate that shape 2D deposited on Si substrates is well preserved upon irradiation by modeling implantation, lithography, and sputtering conditions. Structural changes in analyzed using AFM imaging. The observed effects include structure height decrease or...
Rapid progress in the development of highly efficient nanoparticle-based construction technologies has not always been accompanied by a corresponding understanding their effects on human health and ecosystems. Here, we compare toxicological pristine TiO2, ZnO SiO2, coated SiO2 nanoparticles evaluate suitability as additives to consolidants weathered materials. First, WST-1 LDH assays were used determine viability alveolar A549 cells at various nanoparticle concentrations (0–250...
D-alanyl-D-alanine carboxypeptidase, product of dacD gene in Francisella, belongs to penicillin binding proteins (PBPs) and is involved remodeling newly synthetized peptidoglycan. In E. coli, PBPs are various growth phases they able substitute each other a certain extent. The DacD protein was found be accumulated fraction enriched membrane from severely attenuated dsbA deletion mutant strain. It has been presumed that the DsbA not virulence factor by itself but its substrates, whose correct...
Lipophosphonoxins (LPPOs) are small modular synthetic antibacterial compounds that target the cytoplasmic membrane. First-generation LPPOs (LPPO I) exhibit an antimicrobial activity against Gram-positive bacteria; however they do not any Gram-negatives. Second-generation II) also broadened We investigated reasons behind this different susceptibility of bacteria to two generations using model membranes and living Bacillus subtilis Escherichia coli. show both form oligomeric conductive pores...
Abstract Background Inhalation of lead oxide nanoparticles (PbO NPs), which are emitted to the environment by high-temperature technological processes, heavily impairs target organs. These pass through lung barrier and distributed via blood into secondary organs, where they cause numerous pathological alterations. Here, we studied in detail, macrophages as specialized cells involved innate adaptive immune response selected organs unravel their potential involvement reaction subchronic PbO NP...
Nanodiamonds coated with a random cationic copolymer based on (2-dimethylaminoethyl) methacrylate (DMAEMA) and N -(2-hydroxypropyl) methacrylamide (HPMA) enable highly effective cellular delivery of siRNAs. Clathrin-mediated endocytosis is the predominant entry mechanism.
ABSTRACT Gene silencing using small interfering RNAs (siRNAs) is a selective and promising approach for treatment of numerous diseases. However, broad applications siRNAs are compromised by their low stability in biological environment limited ability to penetrate cells. Nanodiamonds (NDs) coated with cationic polymers can enable cellular delivery siRNAs. Recently, we developed new type ND coating based on random copolymer consisting (2-dimethylaminoethyl) methacrylate (DMAEMA)...
Journal Article An Alternative Approach to Cryo-FIB Lift-out Using a Novel Cooled Nanomanipulator Get access Samuel Záchej, Záchej TESCAN, Brno, Czech Republic Search for other works by this author on: Oxford Academic Google Scholar Dominik Pinkas, Pinkas Institute of Molecular Genetics the Academy Sciences, Prague, Martina Zánová, Zánová Vlada Filimonenko, Filimonenko Jakub Javůrek, Javůrek Corresponding author: jakub.javurek@tescan.com Rostislav Váňa Microscopy and Microanalysis, Volume...