A5051376268- Antimicrobial Peptides and Activities
- Orthopedic Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Hops Chemistry and Applications
- Antimicrobial Resistance in Staphylococcus
- Porphyrin and Phthalocyanine Chemistry
- Orthopaedic implants and arthroplasty
- Nanoplatforms for cancer theranostics
- Photodynamic Therapy Research Studies
- Oxidative Organic Chemistry Reactions
- Peroxisome Proliferator-Activated Receptors
- Adsorption, diffusion, and thermodynamic properties of materials
- Hormonal and reproductive studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Diatoms and Algae Research
- Antimicrobial agents and applications
- Synthesis and Biological Evaluation
- RNA and protein synthesis mechanisms
- Fermentation and Sensory Analysis
- Cholesterol and Lipid Metabolism
- Powdery Mildew Fungal Diseases
- Nosocomial Infections in ICU
- Bee Products Chemical Analysis
- Endodontics and Root Canal Treatments
- Total Knee Arthroplasty Outcomes
Palacký University Olomouc
2013-2024
Institute of Molecular and Translational Medicine
2012-2024
University Hospital Olomouc
2021-2022
Ocní klinika
2013
Russian Academy of Sciences
1975-1983
The resistance of bacteria towards traditional antibiotics currently constitutes one the most important health care issues with serious negative impacts in practice. Overcoming this issue can be achieved by using antibacterial agents multimode action. Silver nano-particles (AgNPs) are well-known substances showing such Therefore, AgNPs suitable candidates for use combinations order to improve their In work, a systematic study quantifying synergistic effects different modes action and...
Photodynamic therapy is usually used against malignant and non-malignant tumors. Nowadays, due to resistance of bacterial strains, we are looking for a new antimicrobial strategy destroy bacteria with minimal invasive consequences. The worldwide increase in antibiotic among different classes gram-positive gram-negative has led the search alternative anti-microbial therapies such as PDT (aPDT). Development technology combines nontoxic compound, called photosensitizer, visible light...
A series of 80 7-(het)aryl- and 7-ethynyl-7-deazapurine ribonucleosides bearing a methoxy, methylsulfanyl, methylamino, dimethylamino, methyl, or oxo group at position 6, 2,6-disubstituted derivatives methyl amino 2, were prepared, the biological activity compounds was studied compared with that parent 7-(het)aryl-7-deazaadenosine series. Several compounds, in particular 6-substituted 7-deazapurine furyl ethynyl 7, significantly cytotoxic low nanomolar concentrations whereas most much less...
Hops are a well-known source of resins, essential oils and polyphenolic substances, such as proanthocyanidins or prenylflavonoids with significant representatives xanthohumol, isoxanthohumol, 8-prenylnaringenine, represent an ingredient in beer production.Recently, however, many additional bioactive effects hop compounds have been investigated.A systematic review the structure-function relationship between individual hop-derived their bio-activity has lacking.In this we summarize some recent...
The increase in the number of bacterial strains resistant to known antibiotics is alarming. In this study we report synthesis novel compounds termed Lipophosphonoxins II (LPPO II). We show that LPPO display excellent activities against Gram-positive and -negative bacteria, including pathogens multiresistant strains. describe their mechanism action-plasmatic membrane pore-forming activity selective for bacteria. Importantly, neither damage nor cross eukaryotic plasmatic at bactericidal...
Azole antifungal ketoconazole (KET) was demonstrated to activate aryl hydrocarbon receptor (AhR). Since clinically used KET is a racemic mixture of two cis-enantiomers (2R,4S)-(+)-KET and (2S,4R)-(−)-KET, we examined the effects enantiomers on AhR signaling pathway. (+)-KET dose-dependently activated in human gene reporter cell line AZ-AHR, displayed 5–20× higher agonist activity (efficacy), as compared (−)-KET; both were antagonists with equal potency (IC50). Consistently, strongly induced...
The results of studies the catalytic action metal complexes porphines and azaporphines in reactions involving oxidation by molecular oxygen are surveyed. Various mechanisms metal-porphine catalysts analysed a relation between characteristics structures these compounds spectrum their activity is demonstrated. In terms problem preparation new for selective organic under mild conditions, possible ways seeking systems which metal-porphines function as transferring agents outlined. bibliography...
Novel compounds termed lipophosphonoxins were prepared using a simple and efficient synthetic approach. The general structure of consists four modules: (i) nucleoside module, (ii) an iminosugar (iii) hydrophobic module (lipophilic alkyl chain), (iv) phosphonate linker that holds together modules i-iii. Lipophosphonoxins displayed significant antibacterial properties against panel Gram-positive species, including multiresistant strains. minimum inhibitory concentration (MIC) values the best...
Bacterial resistance to antibiotics is a constantly growing challenge. Photodynamic therapy (PDT) offers new approach the treatment of bacterial and viral diseases. The aim this study was compare efficacy photosensitizers used in PDT applied cell lines strains.We tested cytotoxicity phototoxicity 3 photosensitizers: TPPS4, ZnTPPS4 TMPyP NIH3T3 line using two established methods for measuring ROS production and, MTT viability assay. determined spectrophotometrically over 24 h following...
Accurate and rapid diagnosis of prosthetic joint infection (PJI) is vital for rational effective therapeutic management this condition. Several diagnostic strategies have been developed discriminating between infected noninfected cases. However, none them can reliably diagnose the whole spectrum clinical presentations PJI. Here, we report a new method PJI detection based on magnetically assisted surface enhanced Raman spectroscopy (MA-SERS) using streptavidin-modified magnetic nanoparticles...
The advantages offered by established antibiotics in the treatment of infectious diseases are endangered due to increase number antibiotic-resistant bacterial strains. This leads a need for new antibacterial compounds. Recently, we discovered series compounds termed lipophosphonoxins (LPPOs) that exhibit selective cytotoxicity towards Gram-positive bacteria include pathogens and resistant For further development these compounds, it was necessary identify mechanism their action characterize...
Our research focused on the antimicrobial effects of purified hop (Humulus lupulus L.) fractions including α-bitter acids (humulones), β-bitter (lupulones) and xanthohumol, a commercial CO
The alarming rise of bacterial antibiotic resistance requires the development new compounds. Such compounds, lipophosphonoxins (LPPOs), were previously reported to be active against numerous species, but serum albumins abolished their activity. Here we describe synthesis and evaluation novel antibacterial compounds termed LEGO-LPPOs, loosely based on LPPOs, consisting a central linker module with two attached connector modules either side. are then decorated polar hydrophobic modules. We...
Successful surgeries involving orthopedic implants depend on the avoidance of biofilm development implant surface during early postoperative period. Here, we investigate potential novel antibacterial compounds-second-generation lipophosphonoxins (LPPOs II)-as additives to surgical bone cements. We demonstrate (i) excellent thermostability LPPOs II, which is essential withstand elevated temperatures exothermic cement polymerization; (ii) unchanged tensile strength and elongation at break...
Hospitalized patients with wounds face an increased risk of infection multi-drug-resistant nosocomial bacteria. In this study, samples from almost 10,000 big hospitals in Czech Republic infected were analyzed for the presence bacterial pathogens. 7693 (78.8%), etiological agents identified. Members Enterobacterales (37.1%) and Staphyloccus aureus (21.1%) most prevalent showed methicillin resistance 8.6%. Almost half Klebsiella pneumoniae isolates ESBL-positive 25.6% Enterobacter spp....
Lipophosphonoxins (LPPOs) are small modular synthetic antibacterial compounds that target the cytoplasmic membrane. First-generation LPPOs (LPPO I) exhibit an antimicrobial activity against Gram-positive bacteria; however they do not any Gram-negatives. Second-generation II) also broadened We investigated reasons behind this different susceptibility of bacteria to two generations using model membranes and living Bacillus subtilis Escherichia coli. show both form oligomeric conductive pores...
Broad-spectrum antibiotics administered to patients with severe COVID-19 pneumonia pose a risk of infection caused by Clostridioides difficile. This is reduced mainly strict hygiene measures and early de-escalation antibiotic therapy. Recently, oral vancomycin prophylaxis (OVP) has also been discussed. retrospective study aimed assess the prevalence C. difficile in critical staying an intensive care unit tertiary hospital department anesthesiology, resuscitation, from November 2020 May 2021...
(1) Background: Microorganisms originating from the microflora of oral cavity are main cause inflammatory diseases dental pulp and periapical periodontium, as well failure endodontic treatment. The subsequent root canal treatment is not able to remove all pathogens, a small number viable bacteria remain in dentine tubules, which must be sealed by sealers. These sealers should have at least bacteriostatic effect prevent remaining reproducing. aim this study compare short-term antibacterial...