A5059582565- Toxic Organic Pollutants Impact
- Carcinogens and Genotoxicity Assessment
- Effects and risks of endocrine disrupting chemicals
- Cancer-related Molecular Pathways
- Pharmacogenetics and Drug Metabolism
- Estrogen and related hormone effects
- Air Quality and Health Impacts
- Cancer, Lipids, and Metabolism
- Neonatal Respiratory Health Research
- Heme Oxygenase-1 and Carbon Monoxide
- Immune Response and Inflammation
- Connexins and lens biology
- Epigenetics and DNA Methylation
- Environmental Toxicology and Ecotoxicology
- Cancer, Hypoxia, and Metabolism
- Genomics, phytochemicals, and oxidative stress
- Inflammatory mediators and NSAID effects
- Neonatal Health and Biochemistry
- Environmental Science and Water Management
- Free Radicals and Antioxidants
- Drug Transport and Resistance Mechanisms
- Metabolism and Genetic Disorders
- Retinoids in leukemia and cellular processes
- Lipid metabolism and biosynthesis
- DNA Repair Mechanisms
Czech Academy of Sciences, Institute of Biophysics
2016-2025
Czech Academy of Sciences
2005-2022
Prague University of Economics and Business
2020
Masaryk University
1997-2016
Cytokinetics (United States)
2016
Veterinary Research Institute
2006-2015
University of Chemistry and Technology, Prague
2014
Czech Academy of Sciences, Institute of Experimental Medicine
2008
Czech Academy of Sciences, Institute of Mathematics
2006
University of Iowa
2004
The toxic equivalency concept used for the risk assessment of polychlorinated biphenyls (PCBs) is based on aryl hydrocarbon receptor (AhR)–mediated toxicity coplanar dioxin-like (DL) PCBs. Most PCBs in environment, however, are non-dioxin-like (NDL) that cannot adopt a structure required AhR activation. For NDL-PCBs, no generally accepted available because their insufficiently characterized. Here, we systematically determined vitro profiles 24 regarding 10 different mechanisms action. Prior...
We examined the effects of gut microbial catabolites tryptophan on aryl hydrocarbon receptor (AhR). Using a reporter gene assay, we show that all studied are low-potency agonists human AhR. The efficacy differed substantially, comprising with no or low (i3-propionate, i3-acetate, i3-lactate, i3-aldehyde), medium (i3-ethanol, i3-acrylate, skatole, tryptamine), and high (indole, i3-acetamide, i3-pyruvate) efficacies. displayed ligand-selective antagonist activities by i3-pyruvate, i3-aldehyde,...
Polychlorinated biphenyls (PCBs) are thought to cause numerous adverse health effects, but their impact on estrogen signaling is still not fully understood. In the present study, we used ER-CALUX bioassay determine estrogenic/antiestrogenic activities of prevalent PCB congeners and mixtures isolated from human male serum. The samples were collected residents an area with extensive environmental contamination a former production site as well neighboring background region in eastern Slovakia....
Sediment extracts from three polluted sites of the river Elbe basin were fractionated using a novel online fractionation procedure. Resulting fractions screened for mutagenic, aryl hydrocarbon receptor (AhR)-mediated, transthyretin (TTR)-binding, and estrogenic activities their potency to inhibit gap junctional intercellular communication (GJIC) compare toxicity patterns identify priority fractions. Additionally, more than 200 compounds compound classes identified GC-MS/MS, LC-MS/MS,...
β-Catenin is a key integrator of cadherin-mediated cell-cell adhesion and transcriptional regulation through the Wnt/β-catenin pathway, which plays an important role in liver biology. Using model contact-inhibited progenitor cells, we examined interactions signaling with aryl hydrocarbon receptor (AhR), ligand-activated transcription factor, mediates toxicity dioxin-like compounds, including their effects on development hepatocarcinogenesis. We found that AhR cooperated induction targets,...
Polycyclic aromatic hydrocarbons (PAHs), such as benzo[a]pyrene (BaP), are carcinogens suggested to be involved in development of human cancer. Several recent studies have reported that PAHs can activate estrogen receptors (ER), either directly or indirectly by producing estrogenic metabolites. We hypothesized the activation ER their metabolites could induce cell proliferation estrogen-sensitive cells. In present study, we found two PAHs, benz[a]anthracene (BaA) and BaP, stimulate breast...
Under Global Initiative for Asthma guidelines, the clinical control of disease activity and adjustment treatment in patients with asthma are based on symptoms, use rescue medication, lung function peak expiratory flow measurement (standard strategy). We investigated whether a strategy to reduce number sputum eosinophils (EOS strategy) gives better lower exacerbation rate compared standard strategy. Fifty-five moderate severe entered this open, randomized, parallel-group study visited...
In the present study, a series of 32 hydroxy- and dihydroxy-polychlorinated biphenyls (OH-PCBs) PCB-derived quinones were prepared evaluated for their in vitro potencies to downregulate gap junctional intercellular communication (GJIC) activate aryl hydrocarbon receptor (AhR) estrogen alpha (ER) well-established liver mammary cell models. The rat epithelial line WB-F344 was used determination GJIC inhibition; AhR-inducing activity determined hepatoma H4IIE.Luc cells stably transfected with...
Extracts of sediment samples collected from the Morava River and its tributaries (Czech Republic) were examined for mutagenic, dioxin-like, estrogenic activities. Moreover, human leukemic HL-60 cell line was tested as a potential model detection effects environmental contaminants on proliferation differentiation processes. Analytical data indicate that sediments contaminated predominantly with polycyclic aromatic hydrocarbons (PAHs) phthalate esters. The sums concentrations 16 U.S....