A5032794113- Lipid Membrane Structure and Behavior
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
- Probiotics and Fermented Foods
- Escherichia coli research studies
- Nanopore and Nanochannel Transport Studies
- Ocular Surface and Contact Lens
- RNA and protein synthesis mechanisms
- Antimicrobial agents and applications
- Neonatal Respiratory Health Research
- Advanced Drug Delivery Systems
- Vibrio bacteria research studies
- Pancreatic function and diabetes
- Cellular transport and secretion
- Biochemical Analysis and Sensing Techniques
- Grouting, Rheology, and Soil Mechanics
- Antibiotic Resistance in Bacteria
- Pharmacological Effects of Natural Compounds
- Inhalation and Respiratory Drug Delivery
- Gut microbiota and health
- Yersinia bacterium, plague, ectoparasites research
- Fungal Biology and Applications
- Molecular Sensors and Ion Detection
Charles University
2019-2025
Czech Academy of Sciences, J. Heyrovský Institute of Physical Chemistry
2019-2022
Abstract Colicin production in Escherichia coli ( E . ) strains represents an important trait with regard to microbial survival and competition the complex intestinal environment. A novel colicin type, Z (26.3 kDa), was described as a product of original producer, extraintestinal B1356 strain, isolated from anorectal abscess 17 years-old man. The 4,007 bp plasmid (pColZ) completely sequenced activity cza immunity czi genes were identified. are transcribed opposite directions encode for 237...
We developed modular antimicrobial compounds capable of inducing cytoplasmic membrane perforation. This structure–activity relationship study focuses on the role length their alkenyl hydrophobic module.
Abstract Finding effective antibiotics against multi-resistant strains of bacteria has been a challenging race. Linker-Evolved-Group-Optimized-Lipophosphonoxins (LEGO-LPPOs) are small modular synthetic antibacterial compounds targeting the cytoplasmic membrane. Here we focused on understanding reasons for variable efficacy selected LEGO-LPPOs ( LEGO-1 , LEGO-2 LEGO-3 and LEGO-4 ) differing in hydrophobic linker module structure length. LEGO-1–4 permeabilized membrane Staphylococcus aureus...
We introduce and study a multicomponent lipid film mimicking composition of the human lung surfactant. It consists phospholipids with various headgroups tail saturation. Furthermore, it includes cholesterol oxidized lipids. Langmuir trough fluorescence microscopy experiments are combined fully atomistic molecular dynamics simulations. The considered mixtures form complex interfacial films properties modulated by lateral compression. Cholesterol laterally condenses, lipids expand films; both...
Colicin U is a protein produced by the bacterium Shigella boydii (serovars 1 and 8). It exerts antibacterial activity against strains of enterobacterial genera Escherichia Here, we report that colicin forms voltage-dependent pores in planar lipid membranes; its single-pore conductance was found to be about 22 pS M KCl at pH 6 under 80 mV asolectin bilayers. In agreement with high degree homology between their C-terminal domains, shares some pore characteristics related colicins A B. are...
Fonticins are phage tail-like bacteriocins produced by the Gram-negative bacterium Pragia fontium from family Budviciaceae. This produces contractile-type particles that adsorb on surface of sensitive bacteria and penetrate cell wall, probably during contraction, in a way similar to type VI secretion system. We characterized pore-forming activity fonticins using both living cells vitro model membranes. Using potassium leakage assay, we show able permeabilize cells. On black lipid membranes,...
Abstract Linker-Evolved-Group-Optimized-Lipophosphonoxins (LEGO-LPPO) are small synthetic modular peptidomimetics with promising antimicrobial activity. The LEGO-LPPO mechanism of antibacterial action has been determined to be the depolarization and disruption bacterial membranes. Their nature is advantageous for fine tuning their biological properties. In order optimize structure even further, it important understand interaction membranes at molecular level. this work, we present synthesis...
<title>Abstract</title> Finding effective antibiotics against multi-resistant strains of bacteria has been a challenging race. LEGO-lipophosphonoxins (LEGO-LPPOs) are small modular synthetic antibacterial compounds targeting the cytoplasmic membrane. Here we focused on understanding reasons for variable efficacy selected LEGO-LPPOs (LEGO-1, LEGO-2, LEGO-3, and LEGO-4) differing in hydrophobic linker module structure length. LEGO-1-4 permeabilized membrane <italic>Staphylococcus...