A5042054963- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Retinal Development and Disorders
- Protist diversity and phylogeny
- CRISPR and Genetic Engineering
- Nanocluster Synthesis and Applications
- Wnt/β-catenin signaling in development and cancer
- Digestive system and related health
- Diamond and Carbon-based Materials Research
- Photosynthetic Processes and Mechanisms
- Alzheimer's disease research and treatments
- Cancer-related gene regulation
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Nanomaterials in Catalysis
- Supramolecular Self-Assembly in Materials
- Nuclear Structure and Function
- Histone Deacetylase Inhibitors Research
- Cardiomyopathy and Myosin Studies
- Chemical Reactions and Isotopes
- Research on Leishmaniasis Studies
- 14-3-3 protein interactions
- Graphene and Nanomaterials Applications
- Synthesis and biological activity
- Neuroscience and Neuropharmacology Research
Charles University
2004-2024
Max Planck Institute of Immunobiology and Epigenetics
2010
Czech Academy of Sciences
2004-2005
Institute of Molecular Genetics
2005
Czech Academy of Sciences, Institute of Molecular Genetics
2004
The pentameric WASH complex facilitates endosomal protein sorting by activating Arp2/3, which in turn leads to the formation of F-actin patches specifically on surface. It is generally accepted that attaches membrane via interaction its subunit FAM21 with retromer VPS35. However, we observe and present endosomes even absence We show binds surface both a retromer-dependent retromer-independent manner. anchor directly mediated SWIP. Furthermore, SWIP can interact number phosphoinositide...
We conditionally substituted E-cadherin (E-cad; cadherin 1) with N-cadherin (N-cad; 2) during intestine development by generating mice in which an Ncad cDNA was knocked into the Ecad locus. Mutant were born, demonstrating that N-cad can structurally replace E-cad and establish proper organ architecture. After birth, mutant gradually developed a phenotype both small large died at ~2-3 weeks of age, probably due to malnutrition transition solid food. Molecular analysis revealed extended domain...
Abstract Microtubules of all eukaryotic cells are formed by α- and β-tubulin heterodimers. In addition to the well known cytoplasmic tubulins, a subpopulation tubulin can occur in nucleus. So far, potential function nuclear has remained elusive. this work, we show that β-tubulins various organisms contain multiple conserved export sequences, which targets Exportin 1/CRM1 pathway. We demonstrate exemplarily these NES motifs sufficient mediate GFP as model cargo be inhibited leptomycin B, an...
Nanodiamonds coated with a random cationic copolymer based on (2-dimethylaminoethyl) methacrylate (DMAEMA) and N -(2-hydroxypropyl) methacrylamide (HPMA) enable highly effective cellular delivery of siRNAs. Clathrin-mediated endocytosis is the predominant entry mechanism.
Abstract Tau, an axonal microtubule-associated protein, is a critical regulator of microtubule function and stability. Tau interaction with microtubules regulated by tau phosphorylation. hyperphosphorylation implicated in destabilization related to neurodegenerative disorders. How phosphorylation leads however unknown. Recently, it was shown that molecules on cooperatively assemble into cohesive layers termed envelopes. envelopes protect against degradation microtubule-severing enzymes,...
ABSTRACT Gene silencing using small interfering RNAs (siRNAs) is a selective and promising approach for treatment of numerous diseases. However, broad applications siRNAs are compromised by their low stability in biological environment limited ability to penetrate cells. Nanodiamonds (NDs) coated with cationic polymers can enable cellular delivery siRNAs. Recently, we developed new type ND coating based on random copolymer consisting (2-dimethylaminoethyl) methacrylate (DMAEMA)...
Abstract Tau is an intrinsically-disordered microtubule-associated protein (MAP) implicated in neurodegenerative disease. On microtubules, tau molecules segregate into two kinetically distinct phases, consisting of either independently diffusing or interacting that form cohesive “envelopes” around microtubules. Envelopes differentially regulate lattice accessibility for other MAPs, but the mechanism envelope formation remains unclear. Here, we find envelopes cooperatively, locally altering...
We conditionally substituted E-cadherin (E-cad; cadherin 1) with N-cadherin (N-cad; 2) during intestine development by generating mice in which an Ncad cDNA was knocked into the Ecad locus. Mutant were born, demonstrating that N-cad can structurally replace E-cad and establish proper organ architecture. After birth, mutant gradually developed a phenotype both small large died at ~2-3 weeks of age, probably due to malnutrition transition solid food. Molecular analysis revealed extended domain...
Over the past decade, fluorescent gold nanoclusters (AuNCs) have witnessed growing popularity in biological applications and enormous efforts been devoted to their development. In this protocol, a recently developed, facile method for preparation of water soluble, biocompatible, colloidally stable near-infrared emitting AuNCs described detail. This room-temperature, bottom-up chemical synthesis provides easily functionalizable capped with thioctic acid thiol-modified polyethylene glycol...
Over the past decade, fluorescent gold nanoclusters (AuNCs) have witnessed growing popularity in biological applications and enormous efforts been devoted to their development. In this protocol, a recently developed, facile method for preparation of water soluble, biocompatible, colloidally stable near-infrared emitting AuNCs described detail. This room-temperature, bottom-up chemical synthesis provides easily functionalizable capped with thioctic acid thiol-modified polyethylene glycol...
Abstract The pentameric WASH complex facilitates endosomal protein sorting by activating Arp2/3, which in turn leads to the formation of F-actin patches specifically on surface. It is generally accepted that attaches membrane via interaction its subunit FAM21 with retromer VPS35. However, we observe and present endosomes even absence We show binds surface both a retromer-dependent retromer-independent manner. anchor directly mediated SWIP. Furthermore, SWIP can interact number...