A5067776846- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Advanced Fluorescence Microscopy Techniques
- Infectious Aortic and Vascular Conditions
- Tissue Engineering and Regenerative Medicine
- Aortic aneurysm repair treatments
- Pancreatic function and diabetes
- Protein Tyrosine Phosphatases
- Cell Image Analysis Techniques
- Virus-based gene therapy research
- Wireless Body Area Networks
- 3D Printing in Biomedical Research
- Glycosylation and Glycoproteins Research
- Electrospun Nanofibers in Biomedical Applications
Czech Academy of Sciences, Institute of Biotechnology
2023-2025
Charles University
2021-2023
Complex morphogenetic processes such as cell division require a tight coordination of the activities microtubules and actin filaments. There is evidence that anillin, conventionally known an actin-binding bundling protein, regulates microtubule-actin crosstalk during division. However, it unknown whether anillin binds directly to sufficient establish crosslinking between Here we address both questions by developing in-vitro system observe anillin-mediated interactions with filaments dynamic...
MICAL proteins play a crucial role in cellular dynamics by binding and disassembling actin filaments, impacting processes like axon guidance, cytokinesis, cell morphology. Their activity is tightly controlled, as dysregulation can lead to detrimental effects on Although previous studies have suggested that MICALs are autoinhibited, require Rab become active, the detailed molecular mechanisms remained unclear. Here, we report cryo-EM structure of human MICAL1 at nominal resolution 3.1 Å....
Cancer cell invasion through tissue barriers is the intrinsic feature of metastasis, most life-threatening aspect cancer. Detailed observation and analysis cancer behaviour in a 3D environment essential for full understanding mechanisms invasion. The inherent limits optical microscopy resolution do not allow to in-depth intracellular structures, such as invadopodia invading cells. required can be achieved using electron techniques FIB-SEM. However, visualising cells matrix FIB-SEM...
The authors present their contribution to the improvement of methods suitable for detection freezing and thawing damage cells cryopreserved venous grafts used lower limb revascularization procedures. They studied post-thaw viability wall (CVG) immediately after 24 48 h culture at +37 °C in two groups six CVG selected randomly slow refrigerator rapid a water bath °C. were collected from multi-organ tissue brain-dead donors, cryopreserved, stored liquid nitrogen vapor phase five years. was...
The authors review the essential role of modern cryobanks in development cryotechnology before start clinical cell and tissue transplantation programme routine support these programmes within legislative framework settled by European Union directives. Two are presented as examples: autologous allogeneic haematopoietic progenitor (HPC) Czech national cryopreserved vascular (VT) supported cryobank University Hospital Hradec Králové, which is a part Tissue Establishment registered under codes...