A5004885735- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Photonic and Optical Devices
- Semiconductor Lasers and Optical Devices
- Spectroscopy and Laser Applications
- Semiconductor Quantum Structures and Devices
- Monoclonal and Polyclonal Antibodies Research
- Mitochondrial Function and Pathology
- Analytical Chemistry and Sensors
- Advanced Fluorescence Microscopy Techniques
- Laser Design and Applications
- Alzheimer's disease research and treatments
- Nuclear Structure and Function
- Advanced Electron Microscopy Techniques and Applications
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
- Cardiomyopathy and Myosin Studies
- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- Ubiquitin and proteasome pathways
- S100 Proteins and Annexins
- Protein Structure and Dynamics
- Lysosomal Storage Disorders Research
- Micro and Nano Robotics
Czech Academy of Sciences, Institute of Biotechnology
2017-2025
Czech Academy of Sciences
2023-2025
Charles University
2024
Technische Universität Dresden
2013-2019
Max Planck Institute of Molecular Cell Biology and Genetics
2011-2018
Czech Academy of Sciences, Institute of Molecular Genetics
2018
Marie Curie
2009
Goethe University Frankfurt
2009
Ferdinand-Braun-Institut
2002-2006
Fraunhofer Institute for Physical Measurement Techniques
2003-2006
Non-centrosomal microtubule bundles play important roles in cellular organization and function. Although many diverse proteins are known that can bundle microtubules, biochemical mechanisms by which cells could locally control the nucleation formation of understudied. Here, we demonstrate concentration tubulin into a condensed, liquid-like compartment composed unstructured neuronal protein tau is sufficient to nucleate bundles. We show that, under conditions macro-molecular crowding, forms...
The structural and functional domains of Tp50, the human T lymphocyte surface protein associated with E rosette receptor, were probed use two murine monoclonal antibodies. Lysostripping, immune precipitation, competitive binding experiments demonstrated that antibodies 9.6 35.1 bind to Tp50 epitopes in close proximity. In studies, both caused a similar degree antigenic modulation, inhibited cell proliferative responses, cytotoxic function without affecting cells mediate antibody-dependent...
Abstract Intracellular trafficking of organelles, driven by kinesin-1 stepping along microtubules, underpins essential cellular processes. In absence other proteins on the microtubule surface, performs micron-long runs. Under crowding conditions, however, motility is drastically impeded. It thus unclear how acts as an efficient transporter in intracellular environments. Here, we demonstrate that TRAK1 (Milton), adaptor protein for mitochondrial trafficking, activates and increases robustness...
Optineurin (OPTN) mutations are linked to amyotrophic lateral sclerosis (ALS) and normal tension glaucoma (NTG), but a relevant animal model is lacking, the molecular mechanisms underlying neurodegeneration unknown. We find that OPTN C-terminus truncation (OPTN∆C) causes late-onset of retinal ganglion cells (RGCs), optic nerve (ON), spinal cord motor neurons, preceded by decrease axonal mitochondria in mice. discover directly interacts with both microtubules mitochondrial transport complex...
Constriction of the cytokinetic ring, a circular structure actin filaments, is an essential step during cell division. Mechanical forces driving constriction are attributed to myosin motor proteins, which slide filaments along each other. However, in multiple organisms, ring has been reported be independent. How rings constrict absence activity remains unclear. Here, we demonstrate that anillin, non-motor crosslinker, indispensable cytokinesis, autonomously propels contractility bundles....
Laser spectroscopy has found many industrial applications, e.g., control of automotive exhaust and process monitoring. The midinfrared region is special interest because it stronger absorption lines compared to the near infrared (NIR). However, in NIR high quality reliable laser sources, detectors, passive optical components are available. A quantum cascade could change this situation if fundamental advantages can be exploited with compact systems. It will shown that, using pulsed lasers...
The cytoskeleton is a network of interconnected protein filaments, which provide three-dimensional scaffold for cells. Remodeling the important key cellular processes, such as cell motility, division, or morphogenesis. This remodeling traditionally considered to be driven exclusively by processes consuming chemical energy, dynamics filaments action molecular motors. Here, we review two mechanisms cytoskeletal that are independent consumption energy. In both cases directed motion overlapping...
The cytoskeletal motor protein kinesin-1 has been successfully used for many nanotechnological applications. Most commonly, these applications use a gliding assay geometry where substrate-attached proteins propel microtubules along the surface. So far, this only shown to run undisturbed up 8 h. Longer times cause problems like microtubule shrinkage, getting stuck and slowing down. This is particularly problematic in nanofabricated structures total number of limited detachment at structure...
The development of high-power GaAs-based ridge wave guide distributed feedback lasers is described. emit between 760 nm and 980 either in TM or TE polarization. Over a large current range, the exhibit stable operation single transversal longitudinal mode. A maximum continuous-wave output power about 400 mW, spectral linewidth below 1 MHz side mode suppression ratio greater than 50 dB have been demonstrated at room temperature. provided by first second order gratings, formed an...
Form and function of the mitotic spindle depend on motor proteins that crosslink microtubules move them relative to each other. Among these are kinesin-14s, such as Ncd, which interact with one microtubule via their non-processive domains another diffusive tail domains, latter allowing protein slip along surface. Little is known about influence protein's performance. Here, we show anchorage Ncd's impacts velocity force considerably. Tail domain slippage reduced velocities from 270 nm s-1 60...
Complex morphogenetic processes such as cell division require a tight coordination of the activities microtubules and actin filaments. There is evidence that anillin, conventionally known an actin-binding bundling protein, regulates microtubule-actin crosstalk during division. However, it unknown whether anillin binds directly to sufficient establish crosslinking between Here we address both questions by developing in-vitro system observe anillin-mediated interactions with filaments dynamic...
Ciliary microtubule doublets, consisting of an incomplete B-tubule on the surface a complete A-microtubule, form fundamental structural framework motile and sensory cilia/flagella. proteins PACRG FAP20, which are essential for flagellar stability function, localize to junction between A- B-tubule. However, their precise role in doublet assembly dynamics remains unknown. Here, using cell-free assay, TIRF-microscopy, cryo-electron tomography, we demonstrate that combination FAP20 stabilize...
Microtubules assemble from tubulin heterodimers composed of conserved α- and β- tubulins. In many species, including humans, tubulins are expressed multiple genes. While the resulting isotypes show only subtle sequence differences, microtubules made distinct can differ in their dynamic behaviour, as well structure, for example number protofilaments. cells, co-polymerize into mixed-isotype microtubules. How mixing affects microtubule functionality is unknown. Here we that co-polymerization...
Abstract Neurofibromin 1 (NF1) is a tumor suppressor widely mutated across several cancers and primarily recognized as GTPase-activating protein (GAP) that inhibits RAS signaling. Other potential functions have been suggested but still not fully explored, due to its large size complex domain structure. It remains unclear whether somatic mutations in NF1 can be used inform clinical decision-making. We previously showed plays novel RAS-independent role microtubule damage repair response the...
Tip‐tracking of kinesin‐14 motor proteins is believed to be crucial for the assembly and maintenance dynamic microtubule arrays. However, in contrast other members family, H. sapiens HSET has so far never been observed prominently located at plus ends. Here, using an vitro dynamics reconstitution assay we observe tip‐tracking GFP‐HSET presence EB1 (hsEB1). depended on SxIP‐like motif as well EB homology domain hsEB1. D. melanogaster Ncd S. pombe Klp2 assays accompanied by amino acid sequence...