A5017056640- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Microtubule and mitosis dynamics
- Monoclonal and Polyclonal Antibodies Research
- Bacterial Genetics and Biotechnology
- Adipose Tissue and Metabolism
- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Diet and metabolism studies
- Muscle metabolism and nutrition
- Transgenic Plants and Applications
- Toxin Mechanisms and Immunotoxins
- Fungal and yeast genetics research
- Galectins and Cancer Biology
- Photosynthetic Processes and Mechanisms
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
Charles University
2017-2025
Czech Academy of Sciences, Institute of Biotechnology
2021-2024
Objective: The aim of our study was to compare the effects a vegetarian and conventional diet on thigh adipose tissue distribution in subjects with type 2 diabetes (T2D).Methods: Seventy-four T2D were randomly assigned either follow (V, n = 37) or control group who followed an isocaloric anti-diabetic (C, 37). Both diets calorie restricted (−500 kcal/day). To measure insulin sensitivity, hyperinsulinemic (1 mU.kg−1.min−1) isoglycemic clamp conducted. β-Cell function assessed using...
Microtubules (MTs) are dynamically unstable polar biopolymers switching between periods of polymerization and depolymerization, with the switch from to depolymerization phase termed catastrophe reverse transition rescue.1Kirschner M. Mitchison T. Beyond self-assembly: microtubules morphogenesis.Cell. 1986; 45: 329-342https://doi.org/10.1016/0092-8674(86)90318-1Abstract Full Text PDF PubMed Scopus (1076) Google Scholar In presence MT-crosslinking proteins, MTs form parallel or anti-parallel...
Engineered small non-antibody protein scaffolds are a promising alternative to antibodies and especially attractive for use in therapeutics diagnostics. The advantages include smaller size more robust, single-domain structural framework with defined binding surface amenable mutation. This calls systematic approach designing new suitable one or methods of directed evolution. We hereby describe process based on an analysis structures from the Protein Data Bank their experimental examination....
We combined cell-free ribosome display and cell-based yeast selection to build specific protein binders the extracellular domain of human interleukin 9 receptor alpha (IL-9Rα). The target, IL-9Rα, is involved in signalling pathway IL-9, a pro-inflammatory cytokine medically important for its involvement respiratory diseases. successive use modified protocols displays allowed us combine their strengths-the virtually infinite power production (mostly) properly folded soluble proteins display....
Here, we present a previously undescribed approach to modify N-terminal sequences of recombinant proteins increase their production yield in Escherichia coli. Prior research has demonstrated that the nucleotides immediately following start codon can significantly influence protein expression. However, impact these is construct-specific and not universally applicable all proteins. Most previous been limited selecting from few rationally designed sequences. In contrast, used directed...