A5015667185- Immune Cell Function and Interaction
- Fermentation and Sensory Analysis
- Horticultural and Viticultural Research
- Plant Physiology and Cultivation Studies
- T-cell and B-cell Immunology
- Animal Nutrition and Physiology
- Enzyme Production and Characterization
- Rabbits: Nutrition, Reproduction, Health
- Immunotherapy and Immune Responses
- Pancreatic function and diabetes
- Polyamine Metabolism and Applications
- CAR-T cell therapy research
- Animal Diversity and Health Studies
- Amino Acid Enzymes and Metabolism
- Enzyme function and inhibition
- Biopolymer Synthesis and Applications
- Radiation Effects and Dosimetry
- Advanced Cellulose Research Studies
- Enzyme Structure and Function
- Genetic and phenotypic traits in livestock
- Biochemical and Molecular Research
- Livestock Management and Performance Improvement
- Carbohydrate Chemistry and Synthesis
- Lignin and Wood Chemistry
- Phytase and its Applications
Charles University
2006-2025
European Molecular Biology Laboratory
2025
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2018-2020
Czech Academy of Sciences, Institute of Biotechnology
2019
Czech University of Life Sciences Prague
2014-2017
St Anna Children's Hospital
2016
University of Graz
2007
University of Lübeck
2006
Sächsisches Textilforschungsinstitut
1984-1986
Textile Research Institute
1976-1985
The study objective was to investigate the effect of three levels nanoclay minerals (1%, 1.5% and 2%) on growth performance, internal organs blood biochemistry broiler chickens compared vaccines antibiotics. An experiment nine dietary treatments conducted for over than 36 days. Groups 1, 2 3 were fed diet without served as control group (positive with antibiotics (C1), positive only (C2) negative any them (C3). Treatments groups 2, 3, 4, 5 6 same above-mentioned once a week or in weeks. 2%...
Abstract Signaling by the human C-type lectin-like receptor, natural killer (NK) cell inhibitory receptor NKR-P1, has a critical role in many immune-related diseases and cancer. receptors have weak affinities to their ligands; therefore, setting up comprehensive model of NKR-P1-LLT1 interactions that considers state on surface is necessary understand its functions. Here we report crystal structures NKR-P1 NKR-P1:LLT1 complexes, which provides evidence forms homodimers an unexpected...
Human LLT1 is a C-type lectin-like ligand of NKR-P1 (CD161, gene KLRB1 ), receptor natural killer cells. Using X-ray diffraction, the first experimental structures human were determined. Four under various conditions determined: monomeric, dimeric deglycosylated after N -acetylglucosamine unit in two forms and hexameric with homogeneous GlcNAc 2 Man 5 glycosylation. The form follows classical dimerization mode CD69. monomeric keeps same fold exception position an outer part long loop region....
NKp30 is one of the main human natural killer (NK) cell activating receptors used in directed immunotherapy. The oligomerization ligand binding domain depends on length C-terminal stalk region, but our structural knowledge and its role signal transduction remains limited. Moreover, affected by presence type N-glycosylation. In this study, we assessed whether Our results show that forms oligomers when expressed HEK293S GnTI− lines with simple N-glycans. However, was detected only as monomers...
The pathogenic yeast Candida albicans can proliferate in environments with different carbon dioxide concentrations thanks to the carbonic anhydrase CaNce103p, which accelerates spontaneous conversion of bicarbonate and vice versa. Without functional C. cannot survive atmospheric air. CaNce103p falls into β-carbonic class, along its ortholog ScNce103p from Saccharomyces cerevisiae. crystal structure is interest because this enzyme a potential target for surface disinfectants.Recombinant was...
β‐ N ‐acetylhexosaminidase from the fungus Aspergillus oryzae is a secreted extracellular enzyme that cleaves chitobiose into constituent monosaccharides. It belongs to GH 20 glycoside hydrolase family and consists of two N‐glycosylated catalytic cores noncovalently associated with 10‐ kD O‐glycosylated propeptides. We used X‐ray diffraction mass spectrometry determine structure A. isolated its natural source. The three‐dimensional determined refined resolution 2.3 Å revealed this active as...
The heme-based oxygen sensor protein
The experiment was conducted on Isa Brown laying hens to study the effects of sea buckthorn fruit residues hen performance and quality eggs. total number 1440 eighteen-week-old pullets divided into two groups (720 each) including 24 subgroups (30 hens). experimental group fed diets in which 5% wheat replaced by residues. control without any colour additives. Egg production intensity were evaluated seven periods, from 127 322 days age. In our study, a significant effect (P ≤ .05) laid eggs...
The experiment was conducted on 2640 slow-growing (NL-JA-757) broiler chicks divided into three groups with eight replicates of 110 each for period 49 days. control group fed diets without any color additive, while experimental A and B were containing commercial additive (0.05%) sea buckthorn fruit residue 5% feed respectively. Diets BR1 (starter) from day 1 to 21; BR2 (grower) 22 35, BR3 (finisher) 36 49. weighed days 21, 35 final mean live weight the birds (males females) appeared lower in...
A phylogenetic analysis of the Lecanora rupicola group based on combined nITS rDNA and β-tubulin sequences a dataset ITS, partial polyketide synthase genes reveals previously unrecognized species, which here is introduced under name bicinctoidea. The new species sister L. swartzii complex (including lojkaeana), characterized by eucorticate ascomata, morphological diversity that includes also dwarf-fruticose lineage. preferential occurrence vertical to overhanging siliceous rocks corresponds...
Abstract Working at the border between innate and adaptive immunity, natural killer (NK) cells play a key role in immune system by protecting healthy eliminating malignantly transformed, stressed or virally infected cells. NK cell recognition of target is mediated receptor “zipper” consisting various activating inhibitory receptors, including C-type lectin-like receptors. Among this major group two largest rodent families are NKR-P1 Clr families. Although these have been shown to encode...
Targeted cancer immunotherapy is a promising tool for restoring immune surveillance and eradicating cells. Hydrophilic polymers modified with coiled coil peptide tags can be used as universal carriers designed cell-specific delivery of such biologically active proteins. Here, we describe the preparation pHPMA-based copolymer conjugated immunologically protein B7-H6 via complementary VAALEKE (peptide E) VAALKEK K) sequences. Receptor was described binding partner NKp30, its expression has...