A5060558684- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Carbohydrate Chemistry and Synthesis
- Enzyme Structure and Function
- Enzyme Production and Characterization
- Maritime Navigation and Safety
- RNA Interference and Gene Delivery
- Infrastructure Resilience and Vulnerability Analysis
- Protein purification and stability
- Network Security and Intrusion Detection
- CAR-T cell therapy research
- Maritime Security and History
- Optimization and Search Problems
- Immune Response and Inflammation
- Vehicle Routing Optimization Methods
- Toxin Mechanisms and Immunotoxins
- Diamond and Carbon-based Materials Research
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Catalysis and Immobilization
- Military Defense Systems Analysis
- Maritime Ports and Logistics
- Information and Cyber Security
Charles University
2015-2025
Czech Technical University in Prague
2010-2015
Czech Academy of Sciences
2008-2014
Czech Academy of Sciences, Institute of Microbiology
2008-2014
Institute of Technology of Cambodia
2010
Département de Chimie Moléculaire
2010
Université Joseph Fourier
2010
Centre National de la Recherche Scientifique
2010
Freie Universität Berlin
2007
Slovak University of Technology in Bratislava
1976
In response to the Mumbai attacks of 2008, police have started schedule a limited number inspection checkpoints on road network throughout city. Algorithms for similar security-related scheduling problems been proposed in recent literature, but security networked domains when targets varying importance remains an open problem at large. this paper, we cast as attacker-defender zero-sum game. The strategy spaces both players are exponentially large, so requires development novel, scalable...
Abstract Gangliosides, sialylated glycosphingolipids abundant in the nervous system, play crucial roles neurotransmission, interaction with regulatory proteins, cell–cell recognition, and signaling. Altered gangliosides expression has been correlated pathological processes, including cancer, inflammatory disorders, autoimmune diseases. Gangliosides are important endogenous ligands of Siglecs (Sialic acid‐binding immunoglobulin‐type lectins), I‐type lectins mostly expressed by immune cells,...
Color centers in diamonds have shown excellent potential for applications quantum information processing, photonics, and biology. Here we report chemical vapor deposition (CVD) growth of nanocrystalline diamond (NCD) films as thin 5–6 nm with photoluminescence (PL) from silicon-vacancy (SiV) at 739 nm. Instead conventional 4–6 detonation nanodiamonds (DNDs), prepared employed hydrogenated 2 DNDs (zeta = +36 mV) to form extremely dense (∼1.3 × 1013 cm–2), (2 ± 1 nm), smooth (RMS roughness <...
Understanding materials with dimensions down to a few nanometers is of major importance for fundamental science as well prospective applications. Structural transformation and phonon-confinement effects in the nanodiamonds (NDs) have been theoretically predicted below 3 nm size. Here, we investigate effect size on surface chemistry, microscopic structure, Raman scattering high-pressure high-temperature (HPHT) detonation (DNDs) 2–3 nm. The NDs are controlled by annealing air...
Progress in cytokine engineering is driving therapeutic translation by overcoming these proteins' limitations as drugs. The interleukin-2 (IL-2) a promising immune stimulant for cancer treatment but limited its concurrent activation of both pro-inflammatory effector cells and anti-inflammatory regulatory T cells, toxicity at high doses, short serum half-life. One approach to improve the selectivity, safety, longevity IL-2 complexation with anti-IL-2 antibodies that bias towards cell...
We have designed, synthesized, and characterized peptides containing four repeats of the sequences VAALEKE (peptide E) or VAALKEK K). While alone adopt in aqueous solutions a random coil conformation, their equimolar mixture forms heterodimeric coiled coils as confirmed by CD spectroscopy. 5-Azidopentanoic acid was connected to N-terminus peptide E via short poly(ethylene glycol) spacer. The terminal azide group enabled conjugation with synthetic drug carrier based on...
We study the problem of optimal resource allocation for packet selection and inspection to detect potential threats in large computer networks with multiple computers differing importance. An attacker tries harm these targets by sending malicious packets from entry points network; defender thus needs optimally allocate her resources maximize probability detection under network latency constraints.We formulate as a graph-based security game heterogeneous capabilities propose mathematical...
Coiled coils are a common structural motif in many natural proteins that can also be utilized the design and preparation of drug delivery systems for noncovalent connection two macromolecules. In this work, different pairs peptides forming coiled coil hetero-oligomers were designed, synthesized, characterized. While peptide sequences (VAALEKE)4 (VAALKEK)4 predominantly form heterodimers with randomly orientated chains, (IAALESE)2-IAALESKIAALESE IAALKSKIAALKSE-(IAALKSK)2 tend to higher an...
Agent-based techniques have the potential to improve security of international maritime transport threatened by a steep rise piracy. We demonstrated that randomized route-selection strategy resulting from normal two-player game formulation transit problem can decrease number attacks and payoff accumulated pirates. Coordinating movement patrol vessels without central authority requires for semi-cooperative planning coalition formation. With regard existing applications agent-based techniques,...
Fungal β‐ N ‐acetylhexosaminidases are inducible extracellular enzymes with many biotechnological applications. The enzyme from Penicillium oxalicum has unique enzymatic properties despite its close evolutionary relationship other fungal hexosaminidases. It high GalNAcase activity, tolerates substrates the modified N‐acyl group better and some unusual catalytic properties. In order to understand these features, we performed isolation, biochemical enzymological characterization, molecular...
Abstract Background Nitrilases attract increasing attention due to their utility in the mild hydrolysis of nitriles. According activity and gene screening, filamentous fungi are a rich source nitrilases distinct evolution from widely examined bacterial counterparts. However, fungal have been less explored than ones. typically heterogeneous quaternary structures, forming short spirals extended filaments, these features making structural studies difficult. Results A nitrilase was amplified by...
Abstract Signaling by the human C-type lectin-like receptor, natural killer (NK) cell inhibitory receptor NKR-P1, has a critical role in many immune-related diseases and cancer. receptors have weak affinities to their ligands; therefore, setting up comprehensive model of NKR-P1-LLT1 interactions that considers state on surface is necessary understand its functions. Here we report crystal structures NKR-P1 NKR-P1:LLT1 complexes, which provides evidence forms homodimers an unexpected...
Siglecs, sialic-acid-binding immunoglobulin-like lectins, are key immune cell receptors that recognize sialic acid residues on surfaces. Pathogens and tumor cells exploit Siglecs to evade responses modulate immunity, contributing significantly infectious disease cancer pathogenesis. Siglec-7, primarily expressed natural killer (NK) cells, functions as an inhibitory receptor, tightly regulating the activity. This study investigates interaction between Siglec-7 capsular polysaccharide (CPS) of...