A5076044431- Carbohydrate Chemistry and Synthesis
- Antimicrobial agents and applications
- Glycosylation and Glycoproteins Research
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Studies on Chitinases and Chitosanases
- Lysosomal Storage Disorders Research
- Enzyme Production and Characterization
- Synthesis and biological activity
- Synthesis and Biological Evaluation
- Synthesis and Characterization of Heterocyclic Compounds
- Enzyme Catalysis and Immobilization
- Surfactants and Colloidal Systems
- Phenothiazines and Benzothiazines Synthesis and Activities
- Biochemical Analysis and Sensing Techniques
- Amino Acid Enzymes and Metabolism
- Chemical Synthesis and Analysis
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Cancer therapeutics and mechanisms
- Protein Interaction Studies and Fluorescence Analysis
- Polyamine Metabolism and Applications
- Synthesis of heterocyclic compounds
- Click Chemistry and Applications
- Ionic liquids properties and applications
Czech Academy of Sciences, Institute of Microbiology
2016-2024
Czech Academy of Sciences
2014-2024
A.E. Arbuzov Institute of Organic and Physical Chemistry
2011-2022
University of South Bohemia in České Budějovice
2010-2022
Russian Academy of Sciences
2009-2019
National Academy of Sciences of Ukraine
1974-1999
Abstract The synthesis of oligosaccharides using mutant glycosidases has been dynamically developing due to the need for novel carbohydrate‐based materials. Chitooligomers (β‐1→4‐linked oligomers N ‐acetylglucosamine) are bioactive compounds applicable in many industrial and pharmacological areas; however, their accessibility is still rather low. In this work, GH20 β‐ ‐acetylhexosaminidase from fungus Talaromyces flavus was engineered by site‐directed mutagenesis obtain three efficiently...
Structure–activity relationships are important for the design of biocides and sanitizers. During spread resistant strains pathogenic microbes, insights into correlation between structure activity become especially significant. The most commonly used nitrogen-containing compounds; phosphorus-containing ones have been studied to a lesser extent. In present study, broad range sterically hindered quaternary phosphonium salts (QPSs) based on tri-tert-butylphosphine was tested their against...
Fungal β‐ N ‐acetylhexosaminidases are inducible extracellular enzymes with many biotechnological applications. The enzyme from Penicillium oxalicum has unique enzymatic properties despite its close evolutionary relationship other fungal hexosaminidases. It high GalNAcase activity, tolerates substrates the modified N‐acyl group better and some unusual catalytic properties. In order to understand these features, we performed isolation, biochemical enzymological characterization, molecular...
Abstract Background Nitrilases attract increasing attention due to their utility in the mild hydrolysis of nitriles. According activity and gene screening, filamentous fungi are a rich source nitrilases distinct evolution from widely examined bacterial counterparts. However, fungal have been less explored than ones. typically heterogeneous quaternary structures, forming short spirals extended filaments, these features making structural studies difficult. Results A nitrilase was amplified by...
Abstract This work is a structure‐activity relationship study that investigates the influence of nature and amount negative charge in carbohydrate substrates on affinity β‐ N ‐acetylhexosaminidases, stimulation natural killer cells. It describes synthetic procedures yielding novel glycosides are useful immunoactivation. Specifically, we present thorough ability six C‐6 modified ‐acetylhexosaminides (aldehyde, uronate, 6‐ O ‐sulfate, ‐phosphate) to serve as for cleavage glycosylation by...
N-Acetylhexosamine oligosaccharides terminated with GalNAc act as selective ligands of galectin-3, a biomedically important human lectin. Their synthesis can be accomplished by β-N-acetylhexosaminidases (EC 3.2.1.52). Advantageously, these enzymes tolerate the presence functional groups in substrate molecule, such thiourea linker useful for covalent conjugation glycans to multivalent carrier, affording glyconjugates. β-N-Acetylhexosaminidases exhibit activity towards both N-acetylglucosamine...
Two libraries of mono- and dimeric pyrrolidine iminosugars were synthesized by CuAAC (thio)urea-bond-forming reactions from the respective azido/aminohexylpyrrolidine iminosugar precursors. The resulting monomeric compounds screened for inhibition β-N-acetylglucosaminidase Jack beans, plant ortholog human lysosomal hexosaminidases. A selection best inhibitors these was then evaluated against β-N-acetylhexosaminidase B (hHexB) nucleocytoplasmic (hOGA). This evaluation identified a potent (nM)...
Here we describe a complex enzymatic approach to the efficient transformation of abundant waste chitin, byproduct food industry, into valuable chitooligomers with degree polymerization (DP) ranging from 6 11. This method involves three-step process: initial hydrolysis chitin using engineered variants novel fungal chitinase Talaromyces flavus generate low-DP chitooligomers, followed by an extension desired DP high-yielding Y445N variant β-N-acetylhexosaminidase Aspergillus oryzae, achieving...
β-N-Acetylhexosaminidases feature so-called wobbling specificity, which means that they cleave substrates both in gluco- and galacto- configurations, with the activity ratio depending on enzyme source. Here we present new finding fungal β-N-acetylhexosaminidases are able to hydrolyze transfer 4-deoxy-N-acetylhexosaminides high yields. This clearly demonstrates 4-hydroxy moiety at substrate pyranose ring is not essential for binding active site, was also confirmed by molecular docking of...
New 1-cetyl-4-aza-1-azoniabicyclo[2.2.2]octane bromide complexes with copper(II) and lanthanum(III) nitrate were characterized using dynamic light scattering transmission electron microscopy, self-assembly the morphological behavior elucidated. For complex, 3D crystal structure was X-ray diffractometry. These metallosurfactants tested as antitumor agents, a high cytotoxic effect comparable doxorubicin revealed against M-HeLa A-549 cell lines. Both 2 times more active toward MCF-7 line than...
Hyaluronic acid, a naturally occurring carbohydrate biopolymer in human tissues, finds wide application cosmetics, medicine, and material science. Its anionic properties play crucial role its interaction with positively charged macromolecules ions. Among these macromolecules, arginine molecules or polyarginine peptides demonstrate potential drug delivery when complexed hyaluronan. This study aimed to compare elucidate the results of both experimental computational investigations on...