A5083765739- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Escherichia coli research studies
- HIV Research and Treatment
- Pneumonia and Respiratory Infections
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Bacterial Infections and Vaccines
- vaccines and immunoinformatics approaches
- Galectins and Cancer Biology
- Infant Nutrition and Health
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Chemical Synthesis and Analysis
- COVID-19 Clinical Research Studies
- Vaccine Coverage and Hesitancy
- Nanocluster Synthesis and Applications
- Peptidase Inhibition and Analysis
- Gold and Silver Nanoparticles Synthesis and Applications
- Parasites and Host Interactions
- Probiotics and Fermented Foods
- Animal Virus Infections Studies
- Gut microbiota and health
Vrije Universiteit Amsterdam
2019-2025
National Research Council
2021-2025
Amsterdam University Medical Centers
2019-2025
Istituto di Chimica Biomolecolare
2024
Cancer Center Amsterdam
2024
Leiden University
2016-2023
Leiden University Medical Center
2018-2023
Amsterdam UMC Location Vrije Universiteit Amsterdam
2013-2022
Finlay Institute
2020-2022
Consejo Superior de Investigaciones Científicas
2021
Coupling of capsular polysaccharides pathogens to immunogenic protein carriers (conjugate vaccines) improves carbohydrate immune response. Our idea is explore gold nanoclusters as prepare fully synthetic vaccines.Gold glyconanoparticles bearing a tetrasaccharide epitope related the Streptococcus pneumoniae type 14 polysaccharide (Pn14PS), T-helper ovalbumin 323-339 peptide (OVA(323-339)), and D-glucose were prepared by one-pot method. Their immunogenicity was tested in mice. Cytokine levels...
Abstract Glycans are involved in various life processes and represent critical targets of biomedical developments. Nevertheless, the accessibility to long glycans with precise structures remains challenging. Here we report on synthesis consisting [→4)-α-Rha-(1 → 3)-β-Man-(1 ] repeating unit, which relevant O -antigen Bacteroides vulgatus , a common component gut microbiota. The optimal combination assembly strategy, protecting group arrangement, glycosylation reaction has enabled us...
Controlling the global COVID-19 pandemic depends, among other measures, on developing preventive vaccines at an unprecedented pace. Vaccines approved for use and those in development intend to elicit neutralizing antibodies block viral sites binding host's cellular receptors. Virus infection is mediated by spike glycoprotein trimer virion surface via its receptor domain (RBD). Antibody response this important outcome of immunization correlates well with neutralization. Here, we show that...
Abstract The immediate call for translational research in the field of coronavirus disease (COVID-19) pandemic, needs new and unexplored angles to support contribute this important worldwide health problem. aim study is better understand pathogenic mechanisms underlying COVID-19, deciphering carbohydrate-mediated interactions SARS-CoV-2 spike protein. We studied carbohydrate-binding receptors that could be viral entry immune-modulatory responses, we protein with host lung microbiota....
The therapeutic approach for the treatment of HIV infection is based on highly active antiretroviral therapy (HAART), a cocktail drugs. Notwithstanding HAART has shown different drawbacks like toxic side effects and emergence viral multidrug resistance. Nanotechnology offers new tools to improve drug prevention. In this scenario, gold nanoparticles are an interesting chemical tool design prepare smart efficient drug-delivery systems. Here we describe preparation antiviral activity...
The gut microbiota guide the development of host immune system by setting a systemic threshold for activation. Lipopolysaccharides (LPSs) from bacteria are able to trigger and local proinflammatory immunomodulatory responses, this capability strongly relies on their fine structures. Up now, only few LPS structures commensals have been elucidated; therefore, molecular motifs that may be important LPS–mammalian cell interactions at level still obscure. Here, we report full structure isolated...
The zwitterionic Streptococcus pneumoniae serotype 1 polysaccharide (Sp1) is an important anchor point for our immune system to act against streptococcal infections. Antibodies can recognize Sp1 saccharides, and it has been postulated that elicit a T-cell-dependent reaction as be presented by MHC-II molecules. To unravel the molecular mode of action this unique we here describe chemical synthesis set fragments, ranging from 3 12 monosaccharides in length. We outline synthetic approach...
Galactofuranose (Galf) is the five-membered ring form of galactose exclusively found in nonmammalian species, among which several are pathogens. To determine putative role this carbohydrate host–pathogen interactions, we synthesized multivalent gold nanoparticles carrying Galf (Galf-GNPs) and show that they recognized by EB-A2 antibody, widely used to detect Galf-containing galactomannan serum Aspergillosis patients. We demonstrated human monocyte-derived dendritic cells bound Galf-GNPs via...
Dendritic Cells (DCs), the most potent antigen-presenting cells, play a critical role in detection of invading pathogens, which are recognized also by multiple carbohydrate-specific receptors. Among them, DC-SIGN is one best characterized, with high-mannose and Lewis-type glycan specificity. In this study, we present targeting device developed using gold nanoparticles functionalized α-fucosyl-β-alanyl amide. The bound to cellular induced internalization as effectively similar particles...
The "pancarcinoma" Tn antigen (αGalNAc-O-Ser/Thr) is a tumor-associated carbohydrate (TACA) overexpressed on the surface of cancer cells and suitable target for anticancer vaccines. However, TACAs commonly show weak immunogenicity, low in vivo stability, poor bioavailability. To address these issues, development physiologically stable TACA synthetic mimetics novel nanocarriers multivalent display are object intense research. Nanomaterials represent scaffolds to multimerize antigens, but...
Biofunctionalized gold nanoparticles (AuNPs) enable innovative translational research and development in biomedicine. Biomolecules such as peptides, proteins, lipids, carbohydrates can be assembled onto AuNPs to yield nanomaterials with unique properties for applications imaging, photothermal therapy, vaccination strategies, drug delivery. The characterization of functionalized still remains an analytical challenge that normally requires the combination multiple techniques. Laser...
Siglec-10 is an inhibitory I-type lectin selectively recognizing sialoglycans exposed on cell surfaces, involved in several patho-physiological processes. The key role plays the regulation of immune functions has made it a potential target for development immunotherapeutics against broad range diseases. However, crystal structure protein not been resolved time being and atomic description interactions with complex glycans previously unraveled. We present here first insights molecular...
Self-adjuvanting vaccines, wherein an antigenic peptide is covalently bound to immunostimulating agent, have been shown be promising tools for immunotherapy. Synthetic Toll-like receptor (TLR) ligands are ideal adjuvants covalent linking peptides or proteins. We here introduce a conjugation-ready TLR4 ligand, CRX-527, potent powerful lipid A analogue, in the generation of novel conjugate-vaccine modalities. Effective chemistry has developed synthesis ligand as well connection it antigen....
One cause of familial Amyotrophic Lateral Sclerosis (ALS) is a mutation in Super Oxide Dismutase 1 (SOD1) whereby amino acid 93 alanine instead glycine (SOD1-G93A). Transgenic mice expressing human SOD1-G93A pathogenic variant develop motor neuron disease (MND), similar to ALS. Humans with ALS and have elevated production inflammatory cytokines, such as IL-6, which may promote MND. We previously showed that infection the Chlorovirus Acanthocystis turfacea chlorella virus (ATCV-1), encodes...