A5035573232- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Hypothalamic control of reproductive hormones
- Pharmacological Receptor Mechanisms and Effects
- Chemical Synthesis and Analysis
- Forensic Anthropology and Bioarchaeology Studies
- Pharmacological Effects and Assays
- Neurotransmitter Receptor Influence on Behavior
- Bone and Dental Protein Studies
- Pain Mechanisms and Treatments
- Lipid Membrane Structure and Behavior
- Hearing, Cochlea, Tinnitus, Genetics
- Pleistocene-Era Hominins and Archaeology
- Regulation of Appetite and Obesity
- Neurobiology and Insect Physiology Research
- Biochemical Analysis and Sensing Techniques
- Glycosylation and Glycoproteins Research
- Olfactory and Sensory Function Studies
- Cannabis and Cannabinoid Research
- Ion channel regulation and function
- Neuroendocrine regulation and behavior
- Neuroendocrine Tumor Research Advances
- Mass Spectrometry Techniques and Applications
- Cancer, Stress, Anesthesia, and Immune Response
- Archaeology and ancient environmental studies
Centre de Recherches sur la Cognition Animale
2021-2025
Université de Toulouse
2010-2025
Centre National de la Recherche Scientifique
2012-2025
AMIS - Laboratoire d'anthropologie moléculaire et imagerie de synthèse
2016-2024
Université Toulouse III - Paul Sabatier
2009-2023
Institut de Pharmacologie et de Biologie Structurale
2008-2020
ORCID
2020
Institut de Biochimie et Génétique Cellulaires
2006
Inserm
2006
Université Libre de Bruxelles
1993-1998
Selective PCR amplification of human and mouse genomic DNAs with oligonucleotides encoding highly conserved regions the delta-opioid somatostatin receptors generated a DNA probe (hOP01, 761 bp) its murine counterpart (mOP86, 447 bp). hOP01 was used to screen cDNA library from brainstem. A clone (named hORL1) isolated, sequenced found encode protein 370 amino acids whose primary structure displays seven putative membrane-spanning domains G protein-coupled membrane receptor. The hORL1 receptor...
The cloning of several receptors activated by either CC or CXC chemokines and belonging to the G protein-coupled family has been reported recently. In present work, we describe a human gene, named ChemR13, encoding new CC-chemokine receptor. gene encodes protein 352 amino acids with calculated molecular mass 40 600 Da displaying single potential site for N-linked glycosylation. Using set overlapping lambda clones, genomic organisation locus was investigated, demonstrating that ChemR13 is...
A cDNA clone encoding a receptor protein which presents all the characteristics of guanine-nucleotide-binding (G-protein)-coupled was isolated from human brain stem library. The probe used (HGMP08) 600 bp DNA fragment amplified by low-stringency PCR, using genomic as template and degenerate oligonucleotide primers corresponding to conserved sequences amongst known G-protein-coupled receptors. deduced amino acid sequence encodes 472 residues shares 97.3% identity with rat cannabinoid cloned...
Nociceptin (orphanin FQ), the newly discovered natural agonist of opioid receptor-like (ORL1) receptor, is a neuropeptide that endowed with pronociceptive activity in vivo. derived from larger precursor, prepronociceptin (PPNOC), whose human, mouse, and rat genes we have now isolated. The PPNOC gene highly conserved three species displays organizational features are strikingly similar to those preproenkephalin, preprodynorphin, preproopiomelanocortin, precursors endogenous peptides,...
Leukocyte chemoattractants act through a rapidly growing subfamily of G protein-coupled receptors. We report the cloning novel human gene encoding an orphan receptor (ChemR23) related to C3a, C5a and formyl Met-Leu-Phe receptors, more distantly subfamilies chemokine ChemR23 transcripts were found be abundant in monocyte-derived dendritic cells macrophages, treated or not with LPS. Low expression could also detected by reverse transcription-PCR CD4+ T lymphocytes. The was assigned radiation...
Neuropeptide FF (NPFF) has been proposed to play a role in pain modulation, opioid tolerance, and several other physiological processes. However, pharmacological agents that would help define roles for this peptide are still missing. Here we report the discovery of potent selective NPFF receptor antagonist, RF9, can be administered systemically. This compound does not show any effects by itself but block efficiently increase blood pressure heart rate evoked NPFF. When chronically coinjected...
Neuropeptides FF (NPFF) and AF (NPAF) are involved in pain modulation opioid tolerance. These peptides were known to act through uncharacterized G protein‐coupled receptors (GPCR). We describe here, using an aequorin‐based assay as screening tool, that orphan GPCR, previously designated HLWAR77, is a functional high affinity receptor for NPFF related peptides. This further NPFFR. Binding experiments performed with new radioiodinated probe, [ 125 I]‐EYF, derived from the EFW‐NPSF sequence of...
BACKGROUND AND PURPOSE Opiates remain the most effective compounds for alleviating severe pain across a wide range of conditions. However, their use is associated with significant side effects. Neuropeptide FF (NPFF) receptors have been implicated in several opiate‐induced neuroadaptive changes including development tolerance. In this study, we investigated consequences NPFF receptor blockade on acute and chronic stimulation opioid mice by using RF9, potent selective antagonist that can be...
The evolutionary relationships among extinct African hominin taxa are highly debated and largely unresolved, due in part to a lack of molecular data. Even within taxa, it is not always clear, based on morphology alone, whether ranges variation sexual dimorphism versus potentially undescribed taxonomic diversity. For Paranthropus robustus , Pleistocene found only South Africa, both phylogenetic other 1,2 the nature intraspecific 3–6 still disputed. Here we report mass spectrometric (MS)...
To understand how two structurally analogous ligand-receptor systems, the nociceptin/opioid receptor-like 1 (ORL1) and dynorphin A/κ-opioid receptor (KOR1) achieve selectivity, chimeras were generated analyzed. Replacing discrete domains located between N-terminus top of third transmembrane helix KOR1 by homologous ORL1 yields hybrid receptors, which, in comparison with parent KOR1, display up to 300-fold increased affinity but low sensitivity toward nociceptin, unaltered (high) A. These...
Derivatization of biologically active peptides by conjugation with fluorophores or radionuclide-bearing moieties is an effective and commonly used approach to prepare molecular tools diagnostic agents. Whereas lysine, cysteine, N-terminal amino acids have been mostly for peptide conjugation, we describe a new, widely applicable based on the nonclassical bioisosteric replacement guanidine group in arginine functionalized carbamoylguanidine moiety. Four arginine-containing receptor ligands...