A5008476664- S100 Proteins and Annexins
- Bacterial Genetics and Biotechnology
- Chronic Myeloid Leukemia Treatments
- Enzyme Structure and Function
- Redox biology and oxidative stress
- Chronic Lymphocytic Leukemia Research
- Cell death mechanisms and regulation
- Peptidase Inhibition and Analysis
- Sepsis Diagnosis and Treatment
- Caveolin-1 and cellular processes
- Eosinophilic Disorders and Syndromes
- Cancer Research and Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Retinoids in leukemia and cellular processes
- RNA Interference and Gene Delivery
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Vibrio bacteria research studies
- Heat shock proteins research
- Lipid Membrane Structure and Behavior
- Cancer, Lipids, and Metabolism
- Protein Structure and Dynamics
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
Institute of Haematology and Blood Transfusion
2007-2020
Charles University
2000-2012
After reading many 2-DE-based articles featuring lists of the differentially expressed proteins, one starts experiencing a disturbing déjà vu. The same proteins seem to predominate regardless experiment, tissue or species. To quantify occurrence individual in 2-DE experiment reports, we compiled identities identified human, mouse, and rat tissues published three recent volumes Proteomics calculated appearance most predominant dataset. frequently protein is highly abundant glycolytic enzyme...
To find out membrane tolerance strategy to ethanol in Bacillus subtilis that possesses a powerful system of protection against environmental stresses.Cytoplasmic membranes B. were severely affected by even short-term exposure 3% (v/v) ethanol: the growth rate and protein synthesis markedly reduced, no adaptive alterations phospholipids detected. Simultaneously, steady-state DPH fluorescence anisotropy (r(ss)) showed rigidity increased substantially. Analysis phosphoproteome using vitro...
Chronic myeloid leukemia (CML) is a malignant hematopoietic disorder distinguished by the presence of BCR‑ABL1 fused oncogene with constitutive kinase activity. Targeted CML therapy specific tyrosine inhibitors (TKIs) leads to marked improvement in survival patients and their quality life. However, development resistance TKIs remains critical issue for subset patients. The most common cause are numerous point mutations gene, followed less multiple mutation-independent mechanisms. Recently,...
Chronic myeloid leukemia (CML) therapy has markedly improved patient prognosis after introduction of imatinib mesylate for clinical use. However, a subset patients develops resistance to and other tyrosine kinase inhibitors (TKIs), mainly due point mutations in the region encoding domain fused BCR-ABL oncogene. To identify potential therapeutic targets imatinib‑resistant CML cells, we derived imatinib-resistant CML-T1 human cell line clone (CML-T1/IR) by prolonged exposure growth media....
The resistance of malignant cells to chemotherapy calls for the development novel anti-cancer drugs. TNF-related apoptosis-inducing ligand (TRAIL) is a pro-apoptotic cytokine, which selectively induces apoptosis in cells. We derived two TRAIL-resistant HL-60 subclones, HL-60/P1 and HL-60/P2, from TRAIL-sensitive acute promyelocytic leukemia cell line. To identify therapeutically exploitable "weaknesses" that could be used as molecular targets their elimination, we performed proteomic (2-DE)...