A5055710584- Environmental and Agricultural Sciences
- Remote Sensing and Land Use
- Climate variability and models
- Environmental Changes in China
- Climate change and permafrost
- Cryospheric studies and observations
- Protein Kinase Regulation and GTPase Signaling
- Glycosylation and Glycoproteins Research
- Geology and Paleoclimatology Research
- Meteorological Phenomena and Simulations
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Hypoxia, and Metabolism
- Hydrology and Drought Analysis
- Geophysics and Gravity Measurements
- Mass Spectrometry Techniques and Applications
- Oceanographic and Atmospheric Processes
- PI3K/AKT/mTOR signaling in cancer
- GNSS positioning and interference
- Landslides and related hazards
- Epigenetics and DNA Methylation
- Advanced Proteomics Techniques and Applications
- Precipitation Measurement and Analysis
- Soil Moisture and Remote Sensing
- Hemophilia Treatment and Research
- Remote Sensing in Agriculture
University of Toronto
2020-2024
National Center for Climate Change Strategy and International Cooperation
2013-2024
Qingdao National Laboratory for Marine Science and Technology
2018-2023
China Meteorological Administration
2006-2023
Lunenfeld-Tanenbaum Research Institute
2011-2022
Hospital for Sick Children
2017-2022
Mount Sinai Hospital
2011-2021
Sinai Health System
2017-2021
SickKids Foundation
2021
Canada Research Chairs
2021
De novo uridine-diphosphate-N-acetylglucosamine (UDP-GlcNAc) biosynthesis requires glucose, glutamine, acetyl-CoA and uridine, however GlcNAc salvaged from glycoconjugate turnover dietary sources also makes a significant contribution to the intracellular pool. Herein we ask whether regulates nutrient transport intermediate metabolism in C57BL/6 mice by increasing UDP-GlcNAc turn Golgi N-glycan branching. added drinking water showed dose-dependent increase growth of young mice, while mature...
Background Motor proteins from the kinesin-5 subfamily play an essential role in spindle assembly during cell division of most organisms. These motors crosslink and slide microtubules spindle. Kinesin-5 are phosphorylated at a conserved site by Cyclin-dependent kinase 1 (Cdk1) mitosis. Xenopus laevis has also been reported to be Aurora A vitro. Methodology/Principal Findings We investigate here effect these phosphorylations on laevis, called Eg5. find that phosphorylation threonine 937...
Glioblastomas stem-like cells (GSCs) by invading the brain parenchyma, remains after resection and radiotherapy tumoral microenvironment become stiffer. GSC invasion is reported as stiffness sensitive associated with altered N-glycosylation pattern. Glycocalyx thickness modulates integrins mechanosensing, but details remain elusive glycosylation enzymes involved are unknown. Here, we studied association between matrix modulation, migration MGAT5 induced in fibrillar 3D context.To mimic...
The catalytic subunits of protein kinase CK2, CK2α and CK2α′, are closely related to each other but exhibit functional specialization. To test the hypothesis that specific functions CK2α′ mediated by interaction partners, we used yeast two-hybrid system identify CK2α- or CK2α′-binding proteins. We report identification characterization a novel CK2-interacting protein, designated CKIP-1, interacts with CK2α, not in system. CKIP-1 also <i>in vitro</i> is co-immunoprecipitated from cell...
Abstract Despite major advances in mass spectrometry, the detection of phosphopeptides by liquid chromatography with electrospray spectrometry (LC/ES‐MS) still remains very challenging proteomics analysis. Phosphopeptides do not protonate efficiently due to presence one or more acidic phosphate groups, making their difficult. However, other mechanisms also contribute difficulties phosphopeptide analysis LC/ES‐MS. We report here on such undocumented problem: formation phosphopeptide‐metal ion...
Abstract A World Meteorological Organization (WMO) Commission for Climatology international panel was convened to examine and assess the available evidence associated with five weather-related mortality extremes: 1) lightning (indirect), 2) (direct), 3) tropical cyclones, 4) tornadoes, 5) hail. After recommending acceptance of only events after 1873 (the formation predecessor WMO), committee evaluated accepted following “highest (indirect strike) lightning” as 469 people killed in a...
Osteopontin (OPN) is a highly modified protein that found in many tissues and has been associated with variety of physiological pathological processes. Bone OPN potent inhibitor hydroxyapatite crystal formation stimulates bone resorption by osteoclasts; these activities, as well others, are dependent upon phosphorylation the protein. We have used mass spectrometry (MS) to perform comprehensive analysis post-translational modification purified from rat bone. Matrix-assisted laser desorption...
Abstract Aberrant N-glycan Golgi remodeling and metabolism are associated with epithelial–mesenchymal transition (EMT) metastasis in patients breast cancer. Despite this association, the N-glycosylation pathway has not been successfully targeted Here, we show that inhibition of mevalonate fluvastatin, a clinically approved drug, reduces both N-glycan-branching, essential components EMT program tumor metastasis. This indicates novel cross-talk between at endoplasmic reticulum (ER) Golgi....
Histone H3 mutations at amino acids 27 (H3K27M) and 34 (H3G34R) are recurrent drivers of pediatric-type high-grade glioma (pHGG). H3K27M lead to global disruption H3K27me3 through dominant negative PRC2 inhibition, while H3G34R local losses H3K36me3 inhibition SETD2. However, their broader oncogenic mechanisms remain unclear. We characterized the H3.1K27M, H3.3K27M H3.3G34R interactomes, finding that is associated with epigenetic transcription factor changes; in contrast removes a break on...
CKIP-1 is a recently identified interaction partner of protein kinase CK2 with number protein-protein motifs, including an N-terminal pleckstrin homology domain. To test the hypothesis that has role in targeting to specific locations, we examined effects on localization CK2. These studies demonstrated can recruit plasma membrane. Furthermore, domain was found be required for interactions and recruitment In this regard, point mutations abolish membrane compromise addition, replacement...
This work presents a novel scheme to retrieve soil moisture (SM) from the Cyclone Global Navigation Satellite System (CyGNSS) data, which is accomplished by using bagged regression trees (BRT) algorithm with inputs being CyGNSS-derived products, corresponding geolocation, and associated climate type. validated <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">in situ</i> hourly SM data acquired China's automatic observation stations throughout...
Climate change resulted in changes crop growth duration and planting structure, northward movement of region, more severe plant diseases insect pests Northwest China. It caused earlier seeding for spring crop, later autumn accelerated growth, reduced mortality winter crop. To adapt to climate change, measures such as optimization agricultural arrangement, adjustment expansion thermophilic crops, development water-saving agriculture have been taken. Damaging consequences imbalance between...
Protein kinase CK2 is a tetrameric enzyme comprised of two regulatory subunits (CK2β) and catalytic (CK2α and/or CK2α′). The crystal structure dimeric CK2β demonstrated that zinc finger mediates dimerization, therefore we constructed mutant in which cysteine residues 109 114 were mutated to serine. Our objectives examine the effects disrupting subunit on tetramer assembly. Examination this zinc-finger-deficient using yeast two-hybrid assay demonstrates fails form homodimers. In order extend...
Immune responses to factor VIII remain the greatest complication in treatment of severe hemophilia A. Recent epidemiological evidence has highlighted that recombinant produced baby hamster kidney cells is more immunogenic than Chinese ovary cells. Glycosylation differences have been hypothesized influence immunogenicity these synthetic concentrates. In two A mouse models, cell-derived elicited a stronger immune response compared VIII. Furthermore, exhibited accelerated clearance from...
Based on daily precipitation data from 115 climate stations, seasonal and annual their extremes over the Tibetan Plateau its surroundings (TPS) in 1963–2015 are investigated. There exists a clear southeast-northwest gradient extreme but an opposite pattern for consecutive dry days (CDDs). The wet southeast is trending while center northwest 1963–2015. Correspondingly, there drying tendency basins wetting semi-dry summer, which will affect water resources corresponding areas. increase...