A5011105258- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Angiogenesis and VEGF in Cancer
- Renal and related cancers
- Epigenetics and DNA Methylation
- 3D Printing in Biomedical Research
- Reproductive Biology and Fertility
- Congenital heart defects research
- Neurogenesis and neuroplasticity mechanisms
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Cancer, Hypoxia, and Metabolism
- Virus-based gene therapy research
- Genetic Syndromes and Imprinting
- Biomedical Ethics and Regulation
- Viral Infectious Diseases and Gene Expression in Insects
- Pancreatic function and diabetes
- Mast cells and histamine
- RNA Research and Splicing
- Prenatal Screening and Diagnostics
- Polyamine Metabolism and Applications
- Retinal Development and Disorders
- Reproductive biology and impacts on aquatic species
Mount Sinai Hospital
2016-2025
Lunenfeld-Tanenbaum Research Institute
2016-2025
University of Toronto
2016-2025
Sinai Health System
2016-2025
Australian Regenerative Medicine Institute
2015-2025
Monash University
2017-2025
University of Pecs
2003-2024
Magyar Agrár- és Élettudományi Egyetem
2021-2024
Takeda (Austria)
2022-2024
University of Szeged
2011-2024
New therapeutic targets for noncognitive reductions in energy intake, absorption, or storage are crucial given the worldwide epidemic of obesity. The gut microbial community (microbiota) is essential processing dietary polysaccharides. We found that conventionalization adult germ-free (GF) C57BL/6 mice with a normal microbiota harvested from distal intestine (cecum) conventionally raised animals produces 60% increase body fat content and insulin resistance within 14 days despite reduced food...
Several newly generated mouse embryonic stem (ES) cell lines were tested for their ability to produce completely ES cell-derived mice at early passage numbers by <==> tetraploid embryo aggregation. One line, designated R1, produced live offspring which as judged isoenzyme analysis and coat color. These culture-derived animals normal, viable, fertile. However, prolonged in vitro culture negatively affected this initial totipotency of after 14, newborns died birth. one the five subclones...
The trophoblast cell lineage is essential for the survival of mammalian embryo in utero. This specified before implantation into uterus and restricted to form fetal portion placenta. A culture mouse blastocysts or early postimplantation trophoblasts presence fibroblast growth factor 4 (FGF4) permitted isolation permanent stem lines. These lines differentiated other subtypes vitro absence FGF4 exclusively contributed vivo chimeras.
Kidney disease affects over 20 million people in the United States alone. Although causes of renal failure are diverse, glomerular filtration barrier is often target injury. Dysregulation VEGF expression within glomerulus has been demonstrated a wide range primary and acquired diseases, although significance these changes unknown. In glomerulus, VEGF-A highly expressed podocytes that make up major portion between blood urinary spaces. this paper, we show glomerular-selective deletion or...
Abstract Summary: We have used a Prx1 limb enhancer to drive expression of Cre Recombinase in transgenic mice. This regulatory element leads throughout the early bud mesenchyme and subset craniofacial mesenchyme. Crossing murine line carrying this transgene reporter mouse harboring floxed Cre‐reporter cassette revealed that recombinase activity is first observed earliest at 9.5 dpc. By mid stages 10.5 dpc recombination essentially complete all mesenchymal cells limb. Expression was never...
The Cre/loxP system has become an important tool in designing postintegrational switch mechanisms for transgenes mice. power and spectrum of application this depends on transgenic mouse lines that provide Cre recombinase activity with a defined cell type-, tissue-, or developmental stage-specificity. We have developed novel line acts as reporter. mice, designated Z/EG (lacZ/EGFP), express lacZ throughout embryonic development adult stages. excision, however, removes the gene, which activates...
Abstract The developmental potential of embryonic stem (ES) cells versus 3.5 day inner cell mass (ICM) was compared after aggregation with normal diploid embry os and developmentally compromised tetraploid embryos. ES were capable colonizing somatic tissues in chimeras but less efficiently than ICMs the same genotype. When ICM"-"tetraploid IS-tetraploid made, newborns almost all completely ICM-or ES-derived. as judged by GPI isozyme analysis, found yolk sac endoderm trophectoderm lineage....
Brain-derived neurotrophic factor (BDNF) regulates neuronal development and function. However, it has been difficult to discern its role in the adult brain influencing complex behavior. Here, we use a recently developed inducible knockout system show that deleting BDNF broad forebrain regions of mice impairs hippocampal-dependent learning long-term potentiation. We nature this loss during earlier stages causes hyperactivity more pronounced deficits. also demonstrate attenuates actions...
Mouse knockout technology provides a powerful means of elucidating gene function in vivo, and publicly available genome-wide collection mouse knockouts would be significantly enabling for biomedical discovery. To date, published exist only about 10% genes. Furthermore, many these are limited utility because they have not been made or phenotyped standardized ways, freely to researchers. It is time harness new technologies efficiencies production mount high-throughput international effort...
To establish the timing of lineage restriction among endodermal derivatives, we developed a method to label permanently subsets lung precursor cells at defined times during development by using Cre recombinase activate floxed alkaline phosphatase or green fluorescent protein genes under control doxycycline-dependent surfactant C promoter. Extensive complete labeling peripheral lung, thyroid, and thymic epithelia, but not trachea, bronchi, gastrointestinal tract occurred when mice were...