A5058749953- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Retinal Development and Disorders
- Reproductive Biology and Fertility
- Renal and related cancers
- Assisted Reproductive Technology and Twin Pregnancy
- Prenatal Screening and Diagnostics
- 3D Printing in Biomedical Research
- Neurogenesis and neuroplasticity mechanisms
- Ovarian function and disorders
- Reproductive System and Pregnancy
- RNA Interference and Gene Delivery
- Neuroscience and Neural Engineering
- Tissue Engineering and Regenerative Medicine
- Virus-based gene therapy research
- Reproductive Health and Technologies
- Biomedical Ethics and Regulation
- Sperm and Testicular Function
- COVID-19 Impact on Reproduction
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Endometriosis Research and Treatment
- Cancer-related molecular mechanisms research
- Ectopic Pregnancy Diagnosis and Management
- Pelvic floor disorders treatments
Hadassah Medical Center
2015-2024
Hebrew University of Jerusalem
1995-2024
Tel Aviv University
2010-2022
University Medical Center
2012
University Hospital and Clinics
2012
Stornoway Historical Society
2010
United States Nuclear Regulatory Commission
2010
Center for Neuro-Oncology
2010
Monash Institute of Medical Research
2000-2006
Hadassah Academic College
1991-2005
Human embryonic stem (ES) cells are pluripotent that may be used in transplantation medicine. These can induced to differentiate into from the three germ layers both vivo and vitro . To determine whether human ES might rejected after transplantation, we examined cell surface expression of MHC proteins these cells. Our results show very low levels class I (MHC-I) on moderately increase or differentiation. A dramatic induction MHC-I was observed when were treated with IFN-γ but not IFN-α -β....
Human embryonic stem cells differentiate spontaneously in vitro into a range of cell types, and they frequently give rise to with the properties extra-embryonic endoderm. We show here that endogenous signaling by bone morphogenetic protein-2 controls differentiation this lineage. Treatment cultures protein antagonist noggin blocks form induces appearance novel type can neural precursors. These findings indicate key early commitment step human differentiation, conservation developmental...
Human embryonic stem cells (hESCs) may potentially serve as a renewable source of for transplantation. In Parkinson's disease, hESC‐derived dopaminergic (DA) neurons replace the degenerated in brain. Here, we generated highly enriched cultures neural progenitors from hESCs and grafted into striatum Parkinsonian rats. The grafts survived at least 12 weeks, transplanted stopped proliferating, teratomas were not observed. differentiated vivo DA neurons, though low prevalence similar to that...
Human embryonic stem (ES) cells originate from the inner cell mass of blastocyst, and retain in culture properties pluripotent early embryo. The study aim was to determine whether open pulled straw (OPS) vitrification method, which is highly effective for cryopreservation embryos, might be also efficient human ES cells.All clumps that were vitrified by OPS method could recovered upon thawing, gave rise colonies after plating. Vitrified significantly smaller showed an increased level...
Human embryonic stem (hES) cells are pluripotent derived from the inner cell mass of early preimplantation embryo. An efficient strategy for stable genetic modification hES may be highly valuable manipulating in vitro and promote study biology, human embryogenesis, development cell-based therapies. Here, we demonstrate that vectors self-inactivating (SIN) immunodeficiency virus type 1 (HIV-1) tools cells. Transduction by a modified vector SIN HIV-1 containing woodchuck hepatitis regulatory...
Multiple sclerosis (MS) is an immune mediated demyelinating disease of the central nervous system (CNS). A potential new therapeutic approach for MS cell transplantation which may promote remyelination and suppress inflammatory process.We transplanted human embryonic stem cells (hESC)-derived early multipotent neural precursors (NPs) into brain ventricles mice induced with experimental autoimmune encephalomyelitis (EAE), animal model MS. We studied effect NPs on functional pathological...
Abstract Retinal and macular degenerations are a major cause of blindness. Cell transplantation is possible therapeutic approach for the replacement degenerating retinal cells. Here, we studied potential human embryonic stem cells (hESCs) to survive, integrate, differentiate into after intraocular transplantation. Highly enriched cultures neural precursors (NPs) expressing transcripts key regulatory genes development were developed from hESCs. After spontaneous differentiation in vitro, NPs...
Human embryonic stem cells (hESCs) have been directed to differentiate into neuronal using many cell-culture techniques. Central nervous system with clinical importance produced from hESCs. To date, however, there no definitive reports of generation peripheral neurons We used a modification the method Sasai and colleagues for mouse primate elicit differentiation When hESCs are cocultured stromal line PA6 3 weeks, induced that coexpress (a) peripherin Brn3a, (b) tyrosine hydroxylase,...
BackgroundAmyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease that affects motor neurons (MNs). It was shown human astrocytes with mutations in genes associated ALS, like C9orf72 (C9) or SOD1, reduce survival of MNs. Astrocyte toxicity may be related to their dysfunction the release neurotoxic factors.MethodsWe used induced pluripotent stem cell-derived from ALS patients carrying and non-affected donors. We utilized these cells investigate astrocytic neuronal...
Gliomas express many genes that play a role in neural precursor cells (NPCs), but no direct comparison between glioma and stem cell (SC) gene expression profiles has been performed. To investigate the similarities differences gliomas SCs, we compared microRNA (miRNA) signatures of glial tumors, embryonic SCs (ESCs), NPCs, normal adult brains from both human mouse tissues. We demonstrated (regardless their grade) methylcholanthrene-induced shared an miRNA profile is reminiscent NPCs. About...
Pluripotent stem cells may acquire genetic and epigenetic variants during culture following their derivation. At a conference organized by the International Stem Cell Initiative, held at The Jackson Laboratory, Bar Harbor, Maine, October 2016, participants discussed how appearance of such can be monitored minimized and, crucially, significance for safety therapeutic applications these assessed. A strong recommendation from meeting was that an international advisory group should set up to...
Teratoma tumor formation is an essential criterion in determining the pluripotency of human pluripotent stem cells. However, currently there no consistent protocol for assessment teratoma forming ability. Here we present detailed characterization a assay that based on subcutaneous co-transplantation defined numbers undifferentiated embryonic cells (hESCs) with mitotically inactivated feeder and Matrigel into immunodeficient mice. The was highly reproducible 100% efficient when 100,000 hESCs...
Abstract The International Stem Cell Initiative compared several commonly used approaches to assess human pluripotent stem cells (PSC). PluriTest predicts pluripotency through bioinformatic analysis of the transcriptomes undifferentiated cells, whereas, embryoid body (EB) formation in vitro and teratoma vivo provide direct tests differentiation. Here we report that EB assays, analyzed after differentiation under neutral conditions promoting ectoderm, mesoderm, or endoderm lineages, are...