A5064602365- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Biomedical Ethics and Regulation
- 3D Printing in Biomedical Research
- Renal and related cancers
- Neurogenesis and neuroplasticity mechanisms
- Animal Genetics and Reproduction
- Alzheimer's disease research and treatments
- Epigenetics and DNA Methylation
- Mesenchymal stem cell research
- Genetics and Neurodevelopmental Disorders
- Tissue Engineering and Regenerative Medicine
- Gene expression and cancer classification
- Single-cell and spatial transcriptomics
- Spaceflight effects on biology
- Anesthesia and Neurotoxicity Research
- Neuroscience and Neural Engineering
- Bioinformatics and Genomic Networks
- Multiple Sclerosis Research Studies
- Science, Research, and Medicine
- Virus-based gene therapy research
- Congenital heart defects research
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Neuroinflammation and Neurodegeneration Mechanisms
Scripps (United States)
2019-2025
Scripps Institution of Oceanography
2014-2025
Scripps Research Institute
2014-2024
San Diego State University
2022
Torrey Pines Institute For Molecular Studies
2019-2022
University of California, San Diego
2008-2020
AspenTech (United States)
2020
Scripps Health
2019
Mary Ann Liebert (United States)
2016
Sanford Burnham Prebys Medical Discovery Institute
2005-2010
DNA methylation is a critical epigenetic regulator in mammalian development. Here, we present whole-genome comparative view of using bisulfite sequencing three cultured cell types representing progressive stages differentiation: human embryonic stem cells (hESCs), fibroblastic differentiated derivative the hESCs, and neonatal fibroblasts. As reference, compared our maps with methylome map fully adult type, mature peripheral blood mononuclear (monocytes). We observed many notable common...
Neural stem cell (NSC) transplantation represents an unexplored approach for treating neurodegenerative disorders associated with cognitive decline such as Alzheimer disease (AD). Here, we used aged triple transgenic mice (3xTg-AD) that express pathogenic forms of amyloid precursor protein, presenilin, and tau to investigate the effect neural on AD-related neuropathology dysfunction. Interestingly, despite widespread established Aß plaque neurofibrillary tangle pathology, hippocampal rescues...
B lymphocyte differentiation is characterized by an ordered series of Ig gene assembly and expression events. In the majority normal cells, heavy (H) chain genes precedes that light (L) genes. To determine role protein in cell development L rearrangement, we have generated mice cannot assemble H as a result targeted deletion JH segments embryonic stem cells. Mice homozygous for this are devoid slg+ cells bone marrow periphery. these blocked at large, CD43+ precursor stage. However, do ϰ low...
Fragile X syndrome (FXS) is the most common inherited cause of intellectual disability. In addition to cognitive deficits, FXS patients exhibit hyperactivity, attention social difficulties, anxiety, and other autistic-like behaviors. caused by an expanded CGG trinucleotide repeat in 5′ untranslated region Mental Retardation (FMR1) gene leading epigenetic silencing loss expression protein (FMRP). Despite known relationship between FMR1 expansion silencing, modifications observed at locus,...
Down's syndrome (DS), caused by trisomy of human chromosome 21, is the most common genetic cause intellectual disability. Here we use induced pluripotent stem cells (iPSCs) derived from DS patients to identify a role for astrocytes in pathogenesis. astroglia exhibit higher levels reactive oxygen species and lower synaptogenic molecules. Astrocyte-conditioned medium collected causes toxicity neurons, fails promote neuronal ion channel maturation synapse formation. Transplantation studies show...
Comparative genomics studies in primates are restricted due to our limited access samples. In order gain better insight into the genetic processes that underlie variation complex phenotypes primates, we must have faithful model systems for a wide range of cell types. To facilitate this, generated panel 7 fully characterized chimpanzee induced pluripotent stem (iPSC) lines derived from healthy donors. demonstrate utility comparative iPSC panels, collected RNA-sequencing and DNA methylation...
Large-scale collections of induced pluripotent stem cells (iPSCs) could serve as powerful model systems for examining how genetic variation affects biology and disease. Here we describe the iPSCORE resource: a collection systematically derived characterized iPSC lines from 222 ethnically diverse individuals that allows both familial association-based studies. are with high genomic integrity (no or low numbers somatic copy-number variants) determined using high-throughput RNA-sequencing...
Postmortem analysis of brains patients with Alzheimer's disease (AD) has led to diverse theories about the causes pathology, suggesting that this complex involves multiple physiological changes. In an effort better understand variety and integration these changes, we generated a gene expression profile for AD brain. Comparing affected unaffected brain regions in nine controls six cases, showed 118 7050 sequences on broadly representative cDNA microarray were differentially expressed amygdala...
Human embryonic stem (hES) cells originate during an period of active epigenetic remodeling. DNA methylation patterns are likely to be critical for their self-renewal and pluripotence. We compared the status 1536 CpG sites (from 371 genes) in 14 independently isolated hES cell lines with five other types: 24 cancer lines, four adult populations, lymphoblastoid normal human tissues, embryonal carcinoma line. found that profile clearly distinguished from all types. A subset 49 40 genes...
A critical question in Alzheimer's disease (AD) research is the cause of memory loss that leads to dementia. The amyloid precursor protein + presenilin-1 (APP+PS1) transgenic mouse a model for deposition, and like AD, mice develop deficits as deposits accumulate. We profiled gene expression these by microarray quantitative RT-PCR (qRT-PCR). At age when animals developed cognitive dysfunction, they had reduced mRNA several genes essential long-term potentiation formation ( Arc, Zif268, NR2B,...
Abstract Embryonic stem cells are unique among cultured in their ability to self-renew and differentiate into a wide diversity of cell types, suggesting that specific molecular control network underlies these features. Human embryonic (hESCs) known have distinct mRNA expression, global DNA methylation, chromatin profiles, but the involvement high-level regulators, such as microRNAs (miRNA), hESC-specific is poorly understood. We report miRNA expression profiling hESCs variety differentiated...