A5031010935- Prostate Cancer Treatment and Research
- Epigenetics and DNA Methylation
- Renal cell carcinoma treatment
- Multiple and Secondary Primary Cancers
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Cancer, Hypoxia, and Metabolism
- Renal and related cancers
- Cancer, Lipids, and Metabolism
- Radiopharmaceutical Chemistry and Applications
- Prostate Cancer Diagnosis and Treatment
- Gene expression and cancer classification
- Advanced Radiotherapy Techniques
- Pancreatic and Hepatic Oncology Research
- Ferroelectric and Piezoelectric Materials
- Sexual Differentiation and Disorders
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Multiple Myeloma Research and Treatments
- Malaria Research and Control
- Molecular Biology Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Cancer-related molecular mechanisms research
- Microwave Dielectric Ceramics Synthesis
- Cancer-related gene regulation
- Enzyme Structure and Function
West China Hospital of Sichuan University
2014-2025
Sichuan University
2014-2025
Peking University Cancer Hospital
2011-2025
Peking University
2011-2025
Rice University
2024
Zhejiang University
2020-2023
Army Medical University
2015-2023
Southwest Hospital
2015-2023
Affiliated Hospital of North Sichuan Medical College
2023
Hangzhou First People's Hospital
2020-2023
The complete 1.66-megabase pair genome sequence of an autotrophic archaeon, Methanococcus jannaschii, and its 58- 16-kilobase extrachromosomal elements have been determined by whole-genome random sequencing. A total 1738 predicted protein-coding genes were identified; however, only a minority these (38 percent) could be assigned putative cellular role with high confidence. Although the majority related to energy production, cell division, metabolism in M. jannaschii are most similar those...
We have developed a high-throughput method for analyzing the methylation status of hundreds preselected genes simultaneously and applied it to discovery signatures that distinguish normal from cancer tissue samples. Through an adaptation GoldenGate genotyping assay implemented on BeadArray platform, state 1536 specific CpG sites in 371 (one nine per gene) was measured single reaction by multiplexed 200 ng bisulfite-treated genomic DNA. The used obtain quantitative measure level at each site....
The genetic factors underlying common disease arelargely unknown. Discovery of disease-causing genes willtransform our knowledge the contribution tohuman disease, lead to new screens, and underpinresearch into cures improved lifestyles. sequencing human genome has catalyzed efforts tosearch for by strategy associating sequence variants with measurable phenotypes. In particular, Human Genome Project follow-on tocharacterize variation have resulted in discovery millions single-nucleotide...
Bisulfite sequence analysis of individual CpG sites within genomic DNA is a powerful approach for methylation in the genome. The major limitation bisulfite-based methods parallelization. Both array and next-generation sequencing technology are capable addressing this bottleneck. In report, we describe application Infinium® genotyping to analyze bisulfite-converted simultaneously query state over 27,000 from promoters consensus coding sequences (CCDS) genes.We adapted Infinium assay readout...
Chromosome 2 of Plasmodium falciparum was sequenced; this sequence contains 947,103 base pairs and encodes 210 predicted genes. In comparison with the Saccharomyces cerevisiae genome, chromosome has a lower gene density, introns are more frequent, proteins markedly enriched in nonglobular domains. A family surface proteins, rifins, that may play role antigenic variation identified. The complete sequencing shown A+T-rich P. genome is technically feasible.
We have developed a simple and efficient algorithm to identify each member of large collection DNA-linked objects through the use hybridization, applied it manufacture randomly assembled arrays beads in wells. Once has been used determine identity bead, microarray can be wide variety applications, including single nucleotide polymorphism genotyping gene expression profiling. The requires only few labels several sequential hybridizations thousands different DNA sequences with great accuracy....
Human embryonic stem (hES) cells originate during an period of active epigenetic remodeling. DNA methylation patterns are likely to be critical for their self-renewal and pluripotence. We compared the status 1536 CpG sites (from 371 genes) in 14 independently isolated hES cell lines with five other types: 24 cancer lines, four adult populations, lymphoblastoid normal human tissues, embryonal carcinoma line. found that profile clearly distinguished from all types. A subset 49 40 genes...
Acute myeloid leukemia (AML) is a hematological malignancy with an alarming mortality rate. The development of novel therapeutic targets or drugs for AML urgently needed. Ferroptosis form regulated cell death driven by iron-dependent lipid peroxidation. Recently, ferroptosis has emerged as method targeting cancer, including AML. Epigenetic dysregulation hallmark AML, and growing body evidence suggests that subject to epigenetic regulation. Here, we identified protein arginine...
Background Intratumoral oncolytic herpes simplex virus 2-GM CSF (OH2) injection has shown safety and antitumor efficacy in patients with solid tumors. Here, we examined the of OH2 as a single agent or combination HX008, an NMPA-approved PD-1 inhibitor, locally advanced metastatic sarcoma patients. Methods This multicenter, phase 1/2 trial enrolled injectable lesions, who had failed at least 1 more lines standard treatment. Patients were treated three dose levels (10 6 , 10 7 8 CCID 50 /mL)...
We have developed a highly sensitive, specific and reproducible method for microRNA (miRNA) expression profiling, using the BeadArray™ technology. This incorporates an enzyme-assisted specificity step, solid-phase primer extension to distinguish between members of miRNA families. In addition, universal PCR is used amplify all targets prior array hybridization. Currently, assay probes are designed simultaneously analyse 735 well-annotated human miRNAs. Using this method, profiles were...
Background Dl-3-n-butylphthalide (NBP), first isolated from the seeds of celery, showed efficacy in animal models stroke. This study was a clinical trial to assess and safety NBP with continuous dose regimen among patients acute ischemic Methods A randomized, double-blind, double-dummy enrolled 573 within 48 hours onset stroke China. Patients were randomly assigned receive 14-day infusion followed by an capsule, 14- day aspirin, or ozagrel aspirin. The measures Barthel index score modified...
Advances in high-throughput genotyping and the International HapMap Project have enabled association studies at whole-genome level. We constructed panels of over 550,000 (HumanHap550) 650,000 (HumanHap650Y) SNP loci by choosing tag SNPs from all populations genotyped Project. These also contain additional content regions that historically been overrepresented diseases, such as nonsynonymous sites, MHC region, copy number variant mitochondrial DNA. estimate these cover majority common...
Objective To compare the antitumor effect of abiraterone (AA) followed by docetaxel‐prednisone (DP) or vice versa in metastatic castration‐resistant prostate cancer (mCRPC) patients, and explored factors that might predict combined PSA‐PFS, rPFS OS. Patients Methods We retrospectively analyzed mCRPC patients treated with sequential therapy using DP AA versa. who had received enzalutamide cabazitaxel were excluded. The primary outcome measure was overall survival (OS). PSA progression‐free...
Abstract The expression of specific mRNA isoforms may uniquely reflect the biological state a cell because it reflects integrated outcome both transcriptional and posttranscriptional regulation. In this study, we constructed splicing array to examine ∼1,500 from panel genes previously implicated in prostate cancer identified large number type–specific isoforms. We also developed novel “two-dimensional” profiling strategy simultaneously quantify changes transcript abundance; results revealed...
Abstract We employed the BeadArray™ technology to perform a genetic analysis in 33 formalin‐fixed, paraffin‐embedded (FFPE) human esophageal carcinomas, mostly squamous‐cell‐carcinoma (ESCC), and their adjacent normal tissues. A total of 1,432 single nucleotide polymorphisms (SNPs) derived from 766 cancer‐related genes were genotyped with partially degraded genomic DNAs isolated these samples. This directly targeted profiling identified not only previously reported somatic gene...
Objective To determine if prognostic nutritional index (PNI) and its variation could predict initial response to treatment prognosis in metastatic castration‐resistant prostate cancer (mCRPC) patients treated with Abiraterone (AA). Patients Methods One‐hundred‐twelve chemotherapy pretreated or chemotherapy‐naive were scheduled for systemic AA. PNI levels measured before after one month of AA treatment. Univariate multivariate logistic regression analyses used identify predictive factors...
No AccessJournal of UrologyAdult Urology1 Feb 2021Comparative Analysis Genomic Alterations across Castration Sensitive and Resistant Prostate Cancer via Circulating Tumor DNA Sequencing Liancheng Fan, Xiaochen Fei, Yinjie Zhu, Jiahua Pan, Jianjun Sha, Chenfei Chi, Yiming Gong, Xinxing Du, Lixin Zhou, Baijun Dong, Wei Xue FanLiancheng Fan Department Urology, Renji Hospital, School Medicine, Shanghai Jiao Tong University, Shanghai, China , FeiXiaochen Fei ZhuYinjie Zhu PanJiahua Pan ShaJianjun...