A5089144595- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Prostate Cancer Treatment and Research
- Cancer Cells and Metastasis
- RNA modifications and cancer
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Circular RNAs in diseases
- Exercise and Physiological Responses
- Breast Lesions and Carcinomas
- Breast Cancer Treatment Studies
- Cancer Immunotherapy and Biomarkers
- Breast Implant and Reconstruction
- Cancer-related Molecular Pathways
- Lymphoma Diagnosis and Treatment
- PI3K/AKT/mTOR signaling in cancer
- COVID-19 Clinical Research Studies
- Ferroptosis and cancer prognosis
- Prostate Cancer Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Cancer, Lipids, and Metabolism
- Neurofibromatosis and Schwannoma Cases
University of California, San Diego
2015-2024
VA San Diego Healthcare System
2014-2024
United States Department of Veterans Affairs
2015-2021
Veterans Health Administration
2021
VA Desert Pacific Healthcare Network
2020
University of California San Diego Medical Center
1996-2019
Veterans Medical Research Foundation of San Diego
2019
Central Arkansas Veterans Healthcare System
2017
VA Greater Los Angeles Healthcare System
2017
Calgary Laboratory Services
2014
Receptor-tyrosine-kinase-like orphan receptor 1 (ROR1) is expressed during embryogenesis and by certain leukemias, but not normal adult tissues. Here we show that the neoplastic cells of many human breast cancers express ROR1 protein high-level expression in adenocarcinoma was associated with aggressive disease. Silencing cancer cell lines found to this impaired their growth vitro also immune-deficient mice. We could interact casein kinase epsilon (CK1ε) activate phosphoinositide...
Prostate tumors are complex entities composed of malignant cells mixed and interacting with nonmalignant cells. However, molecular analyses by standard gene expression profiling limited because spatial information nontumor cell types lost in sample preparation. We scored 88 prostate specimens for relative content tumor, benign hyperplastic epithelium, stroma, dilated cystic glands. The proportions these were then linked silico to levels determined microarray analysis, revealing unique...
ROR1 is an orphan-receptor tyrosine-kinase-like surface antigen that expressed by many tissues during embryogenesis, some B-cell malignancies, and various cancer cell lines but not virtually all normal adult tissues. Here, we report large proportions of different human cancers also express ROR1, particularly those have high-grade histology. Primary more commonly high levels phosphorylated AKT (p-AKT) cAMP response element binding-factor (p-CREB) than similar lacked expression ROR1. Induced...
Although 1 in 9 American men will receive a diagnosis of prostate cancer (PC), most with this not die from it, as PCs are indolent. However, there is subset patients which the once-indolent PC becomes metastatic and eventually, fatal. In study, we analyzed microbial compositions intratumor bacteria to determine influence microbiome on growth. Using large-scale RNA-sequencing data corresponding clinical data, correlated abundance microbes immune pathways risk factors, identifying specific...
Leukocytosis following exercise is a well-described phenomenon of stress/inflammatory activation in healthy humans. We hypothesized that, despite this increase circulating inflammatory cells, would paradoxically induce expression both pro- and anti-inflammatory cytokines growth factors within these cells. To test hypothesis, 11 adult men, 18–30 yr old, performed 30-min bout heavy cycling exercise; blood sampling was at baseline, end-exercise, 60 min into recovery. The percentage leukocytes...
Abstract More than one million prostate biopsies are performed in the United States every year. A failure to find cancer is not definitive a significant percentage of patients due presence equivocal structures or continuing clinical suspicion. We have identified gene expression changes stroma that can detect tumor nearby. compared profiles 13 containing near and 15 from volunteers without cancer. About 3,800 were found thereafter filtered using independent eliminate possible age-related...
Breast conservation therapy has been the cornerstone of surgical treatment breast cancer for last 20 years; however, recently, use mastectomy increasing. Mastectomy is one most frequently performed operations, and with novel techniques, preservation skin envelope and/or nipple-areolar complex commonly performed. The goal this paper to review literature on skin-sparing nipple-sparing evaluate oncologic safety these techniques. In addition, will discuss importance margin status type as it...
Members of the EGFR/ErbB family tyrosine kinases are found to be highly expressed and deregulated in many cancers, including head neck squamous cell carcinoma (HNSCC). The ErbB family, EGFR, has been demonstrated play key roles metastasis, tumorigenesis, proliferation, drug resistance. Recently, these characteristics have linked a small subpopulation cells classified as cancer stem (CSCs) which believed responsible for tumor initiation maintenance. In this study, we investigated possible...
Head and neck squamous cell carcinoma persists as one of the most common deadly malignancies, with early detection effective treatment still posing formidable challenges. To expand our currently sparse knowledge noncoding alterations involved in disease identify potential biomarkers therapeutic targets, we globally profiled dysregulation small nucleolar long RNAs head tumors. Using next-generation RNA-sequencing data from 40 pairs tumor matched normal tissues, found 2808 RNA (lncRNA)...
In prostate cancer, race/ethnicity is the highest risk factor after adjusting for age. African Americans have more aggressive tumors at every clinical stage of disease, resulting in poorer prognosis and increased mortality. A major barrier to identifying crucial gene activity differences heterogeneity, including tissue composition variation intrinsic histology cancer. We hypothesized that expression specific types would reveal mechanisms involved racial disparities examined 17 pairs arrays...
Protein tyrosine kinases (TKs) are overexpressed in many carcinomas and sarcomas. We studied the expression of following TKs head neck squamous cell carcinoma (HNSCC): platelet-derived growth factor receptor (PDGFR), c-kit, epidermal (EGFR), a serine-threonine kinase, Akt. Formalin-fixed, paraffin-embedded tumor blocks from 44 consecutive patients with primary HNSCC 5 specimens benign pharyngeal laryngeal mucosa were retrieved for immunohistochemical analysis. Of specimens, 38 enough...
Abstract Background Prostate cancer is one of the leading causes illness and death among men in United States world wide. There an urgent need to discover good biomarkers for early clinical diagnosis treatment. Previously, we developed exon-junction microarray-based assay profiled 1532 mRNA splice isoforms from 364 potential prostate related genes 38 tissues. Here, investigate advantage using isoforms, which couple transcriptional splicing regulation, classification. Results As many as 464...
Abstract Objective: To identify the presence of side population (SP) cells in established head and neck squamous carcinoma cell (HNSCC) lines to determine role EGFR regulation these cells. Methods: SP were identified using flow cytometry analysis by ability extrude Hoechst 33342 dye via drug transporter BCRP1/ABCG2. Effect on was determined also difference extrusion immunofluorescence. Immunohistochemical staining performed show BCRP1/ABCG2 phosphorylated form HNSCC tissue. Results: are...
Hollow hard shell particles of 200 nm and 2 micron diameter with a 10 thick porous silica have been synthesized using polystyrene templates sol–gel process. The template ensures than the hollow are monodispersed, while charged surface that they remain suspended in solution for weeks. When filled perfluorocarbon gas, behave as an efficient contrast agent colour Doppler ultrasound imaging human breast tissue. provides unique properties compared to conventional soft employed agents: uniform...
Cancer stem cells (CSC) are believed to play a crucial role in cancer recurrence due their resistance conventional chemotherapy and capacity for self-renewal. Recent studies have reported that salinomycin, livestock antibiotic, selectively targets breast 100-fold more effectively than paclitaxel. In our study we sought determine the effects of salinomycin on head neck squamous cell carcinoma (HNSCC) cells.MTS TUNEL assays were used proliferation apoptosis as function exposure JLO-1, putative...
Alcohol consumption is a well-established risk factor for head and neck squamous cell carcinoma (HNSCC); however, the molecular mechanisms by which alcohol promotes HNSCC pathogenesis progression remain poorly understood. Our study sought to identify microRNAs that are dysregulated in alcohol-associated investigate their contribution malignant phenotype. Using RNA-sequencing data from 136 patients, we compared expression levels of 1,046 between drinking non-drinking cohorts. Dysregulated...
Head and neck squamous cell carcinoma (HNSCC) is an aggressive disease marked by frequent recurrence metastasis stagnant survival rates. To enhance molecular knowledge of HNSCC define a non-coding RNA (ncRNA) landscape the disease, we profiled transcriptome-wide dysregulation long (lncRNA), microRNA (miRNA), PIWI-interacting (piRNA) using RNA-sequencing data from 422 patients in The Cancer Genome Atlas (TCGA). 307 transcripts differentially expressed were significantly correlated with...
The COVID-19 pandemic caused by the SARS-CoV-2 virus, overlaps with ongoing epidemics of cigarette smoking and electronic (e-cig) vaping. However, there is scarce data relating risks outcome or e-cig use. In this study, we mined three independent RNA expression datasets from smokers vapers to understand potential relationship between vaping/smoking dysregulation key genes pathways related COVID-19. We found that smoking, but not vaping, upregulates ACE2, cellular receptor requires for...
Abstract The expression of specific mRNA isoforms may uniquely reflect the biological state a cell because it reflects integrated outcome both transcriptional and posttranscriptional regulation. In this study, we constructed splicing array to examine ∼1,500 from panel genes previously implicated in prostate cancer identified large number type–specific isoforms. We also developed novel “two-dimensional” profiling strategy simultaneously quantify changes transcript abundance; results revealed...
Abstract We employed the BeadArray™ technology to perform a genetic analysis in 33 formalin‐fixed, paraffin‐embedded (FFPE) human esophageal carcinomas, mostly squamous‐cell‐carcinoma (ESCC), and their adjacent normal tissues. A total of 1,432 single nucleotide polymorphisms (SNPs) derived from 766 cancer‐related genes were genotyped with partially degraded genomic DNAs isolated these samples. This directly targeted profiling identified not only previously reported somatic gene...
It is well known that many patients continue to smoke cigarettes after being diagnosed with cancer. Although smoking cessation has typically been presumed possess little therapeutic value for cancer, a growing body of evidence suggests continued associated reduced efficacy treatment and higher incidence recurrence. We therefore investigated the effect cigarette condensate (CSC) on drug resistance in lung cancer head neck cell lines A549 UMSCC-10B, respectively. Our results showed CSC...