A5070624497- Pregnancy and preeclampsia studies
- Prenatal Screening and Diagnostics
- Birth, Development, and Health
- Reproductive System and Pregnancy
- Pluripotent Stem Cells Research
- Renal and related cancers
- Maternal and fetal healthcare
- Gestational Diabetes Research and Management
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Gestational Trophoblastic Disease Studies
- Neonatal Respiratory Health Research
- CRISPR and Genetic Engineering
- Pregnancy and Medication Impact
- Cellular Mechanics and Interactions
- Ectopic Pregnancy Diagnosis and Management
- Grief, Bereavement, and Mental Health
- Cell Adhesion Molecules Research
- Maternal and Perinatal Health Interventions
- Fibroblast Growth Factor Research
- Congenital Diaphragmatic Hernia Studies
- Assisted Reproductive Technology and Twin Pregnancy
- Single-cell and spatial transcriptomics
- Autopsy Techniques and Outcomes
- Cancer-related molecular mechanisms research
University of California, San Diego
2016-2025
Sanford Consortium for Regenerative Medicine
2015-2024
King Edward Memorial Hospital
2023
Michigan Medicine
2023
Manchester Academic Health Science Centre
2023
University of Manchester
2023
Pathwest Laboratory Medicine
2023
Curtin University
2023
La Jolla Alcohol Research
2023
UC San Diego Health System
2016
The pervasive expression of circular RNA is a recently discovered feature gene in highly diverged eukaryotes, but the functions most RNAs are still unknown. Computational methods to discover and quantify essential. Moreover, discovering biological contexts where regulated will shed light on potential functional roles they may play.We present new algorithm that increases sensitivity specificity detection by quantifying linear splicing events at both annotated un-annotated exon boundaries,...
A diagnosis of primary peritoneal serous carcinoma (PPSC) requires exclusion a source in other reproductive organs. Serous tubal intraepithelial (STIC; stage 0) has been described asymptomatic women with BRCA mutations and linked to cancer precursor the fimbria. This study examined frequency STIC PPSC its clinical outcome BRCA-positive women.Presence or absence was recorded consecutive cases meeting 2001 WHO criteria for PPSC, including 26 patients nonuniform sampling fallopian tubes (group...
Down's syndrome (DS), caused by trisomy of human chromosome 21, is the most common genetic cause intellectual disability. Here we use induced pluripotent stem cells (iPSCs) derived from DS patients to identify a role for astrocytes in pathogenesis. astroglia exhibit higher levels reactive oxygen species and lower synaptogenic molecules. Astrocyte-conditioned medium collected causes toxicity neurons, fails promote neuronal ion channel maturation synapse formation. Transplantation studies show...
Comparative genomics studies in primates are restricted due to our limited access samples. In order gain better insight into the genetic processes that underlie variation complex phenotypes primates, we must have faithful model systems for a wide range of cell types. To facilitate this, generated panel 7 fully characterized chimpanzee induced pluripotent stem (iPSC) lines derived from healthy donors. demonstrate utility comparative iPSC panels, collected RNA-sequencing and DNA methylation...
ABSTRACT An increasing body of evidence points to significant spatio-temporal differences in early placental development between mouse and human, but a detailed comparison placentae these two species is missing. We set out compare from both across gestation, with focus on trophoblast progenitor markers. found that CDX2 ELF5, not EOMES, are expressed post-implantation subpopulations species. Genome-wide expression profiling human revealed clusters genes distinct co-expression patterns...
Here, we describe the identification of a novel phosphoprotein named palladin, which colocalizes with α-actinin in stress fibers, focal adhesions, cell–cell junctions, and embryonic Z-lines. Palladin is expressed as 90–92-kD doublet fibroblasts coimmunoprecipitates complex fibroblast lysates. A cDNA encoding palladin was isolated by screening mouse embryo library mAbs. has proline-rich region NH2-terminal half molecule three tandem Ig C2 domains COOH-terminal half. In Northern Western blots...
Group B Streptococcus (GBS) causes invasive infections in human newborns. We recently showed that the GBS β-protein attenuates innate immune responses by binding to sialic acid–binding immunoglobulin-like lectin 5 (Siglec-5), an inhibitory receptor on phagocytes. Interestingly, neutrophils and monocytes also express Siglec-14, which has a ligand-binding domain almost identical Siglec-5 but signals via activating motif, raising possibility these are paired Siglec receptors balance pathogens....
Placental angiogenesis contributes to the pathogenesis of preeclampsia (PE) that affects 5-8% all human pregnancies. MicroRNA (miRNA) are a class noncoding 21- 25-nucleotide RNA negatively regulate gene expression posttranscriptionly.The aim this study was test hypothesis miRNA differentially expressed in healthy term and PE placentas subclass angiogenesis-associated increased by PE.Total were extracted from villous placental tissues severe preeclamptic Differential analyzed microarray...
Significance Human pluripotent stem cells (hPSCs) continue to be underappreciated as a model for studying trophoblast differentiation. In this study, we provide reproducible, two-step protocol by which hPSCs can differentiated into bipotential cytotrophoblast (CTB) stem-like and subsequently functional, terminally trophoblasts. addition, evidence that the response of hPSC-derived CTBs low oxygen is similar primary CTBs. Finally, using trisomy 21-affected hPSCs, show, first time our...
The self-renewal and differentiation capacities of human pluripotent stem cells (hPSCs) make them a promising source material for cell transplantation therapy, drug development, studies cellular development. However, the large numbers necessary many these applications require extensive expansion hPSC cultures, process that has been associated with genetic epigenetic alterations. We have performed combinatorial study on both hESCs hiPSCs to compare effects enzymatic vs. mechanical passaging,...
The placenta is a transient organ that necessary for proper fetal development. Its main functional component the trophoblast, which derived from extra-embryonic ectoderm. Little known about early trophoblast differentiation in human embryo, owing to lack of vitro model system. Human embryonic stem cells (hESCs) differentiate into following BMP4 treatment presence feeder-conditioned media; however, this has not been widely accepted, part proof progenitor population. We have previously shown...
Trophoblast stem cells (TSCs) have recently been derived from human embryos and early-first-trimester placenta; however, aside ethical challenges, the unknown disease potential of these limits their scientific utility. We previously established a bone morphogetic protein 4 (BMP4)-based two-step protocol for differentiation primed pluripotent (hPSCs) into functional trophoblasts; those trophoblasts could not be maintained in self-renewing TSC-like state. Here, we use first step this protocol,...
Fetal thrombotic vasculopathy (FTV) is a placental lesion characterized by regionally distributed avascular villi and often accompanied upstream thrombosis in fetal vessels. Previous studies, using preselected populations, have shown associations of this with adverse neurodevelopmental outcomes potentially obstructive lesions the umbilical cord. We investigated prevalence obstetric complications, perinatal disease, abnormalities cases FTV. One hundred thirteen placentas FTV were identified...
Abnormal trophoblast differentiation and function is the basis of many placenta-based pregnancy disorders, including pre-eclampsia fetal growth restriction. PPARgamma, a ligand-activated nuclear receptor, plays essential roles in placental development; null murine embryos die at midgestation due to abnormalities all layers, particular, small labyrinth expanded giant cell layer. Previous studies have focused mostly on role PPARgamma invasion. Based previously reported preadipocyte...