A5019803298- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- Reproductive Biology and Fertility
- Renal and related cancers
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- Gestational Diabetes Research and Management
- Reproductive System and Pregnancy
- Mesenchymal stem cell research
- Neonatal Respiratory Health Research
- Pancreatic function and diabetes
- Prenatal Substance Exposure Effects
- Cannabis and Cannabinoid Research
- Systemic Lupus Erythematosus Research
- Genetic Syndromes and Imprinting
- Molecular Biology Techniques and Applications
- Fibroblast Growth Factor Research
- Congenital Diaphragmatic Hernia Studies
- Cell Adhesion Molecules Research
- Metabolism, Diabetes, and Cancer
- Kruppel-like factors research
- TGF-β signaling in diseases
- Erythrocyte Function and Pathophysiology
- Sperm and Testicular Function
Queen's University
2019-2025
University of California, San Diego
2014-2023
University of Calgary
2008-2020
John Wiley & Sons (United States)
2018
Triangle
2018
Indianapolis Zoo
2018
Kentucky Science Center
2018
United States University
2017
Western University
2000-2005
Health Sciences Centre
2004-2005
ABSTRACT An increasing body of evidence points to significant spatio-temporal differences in early placental development between mouse and human, but a detailed comparison placentae these two species is missing. We set out compare from both across gestation, with focus on trophoblast progenitor markers. found that CDX2 ELF5, not EOMES, are expressed post-implantation subpopulations species. Genome-wide expression profiling human revealed clusters genes distinct co-expression patterns...
The labyrinth of the rodent placenta contains villi that are site nutrient exchange between mother and fetus. They covered by three trophoblast cell types separate maternal blood sinusoids from fetal capillaries - a single mononuclear is subtype giant (sinusoidal or S-TGC) with endocrine function two multinucleated syncytiotrophoblast layers, each resulting cell-cell fusion, in transport. developmental origins these have not previously been elucidated. We report here discovery...
The objectives were 1) to investigate the effects of oocyte maturation in serum-free and amino acid-supplemented defined media on transcript levels, blastocyst cell number, apoptosis; 2) influence culture atmosphere development, total 3) examine epidermal growth factor (EGF) during number apoptosis. results demonstrate that blastocysts derived from vitro maturation, fertilization, embryo protocols undergo apoptosis but apoptotic levels are not greatly influenced by environment. Amino acid...
1 in 5 women report cannabis use during pregnancy, with nausea cited as their primary motivation. Studies show that (-)-△9-tetrahydrocannabinol (Δ9-THC), the major psychoactive ingredient cannabis, causes fetal growth restriction, though mechanisms are not well understood. Given critical role of placenta to transfer oxygen and nutrients from mother, fetus, any compromise development fetal-placental circulation significantly affects maternal-fetal exchange thereby, growth. The goal this study...
Abstract The placenta is an essential organ that formed during pregnancy and its proper development critical for embryonic survival. While several animal models have been shown to exhibit some of the pathological effects present in human preeclampsia, these often do not represent physiological aspects identified. Hypoxia-inducible factor 1 alpha (Hif-1α) a necessary component cellular oxygen-sensing machinery has implicated as major regulator trophoblast differentiation. Elevated levels...
Cell-cell fusion or syncytialization is fundamental to the reproduction, development, and homeostasis of multicellular organisms. In addition various cell type-specific fusogenic proteins, surface externalization phosphatidylserine (PS), a universal eat-me signal in apoptotic cells, has been observed different events. Nevertheless, molecular underpinnings PS cellular mechanisms PS-facilitated cell-cell are unclear. Here, we report that TMEM16F, Ca2+-activated phospholipid scramblase...
Objective Pregnancies in women with the antiphospholipid syndrome (APS) are frequently complicated by fetal loss and intrauterine growth restriction (IUGR). How circulating antibodies (aPL) cause pregnancy complications APS is poorly understood. We sought to determine whether low‐density lipoprotein receptor family member apolipoprotein E 2 (ApoER2) mediates trophoblast dysfunction induced aPL. Methods Placental ApoER2 expression was evaluated immunohistochemistry immunoblotting. Normal...
Abstract Cannabis use in pregnancy is associated with low birthweight outcomes. Recent preclinical data suggests that maternal Δ9-tetrahydrocannabinol (THC) exposure leads to decreases followed by early cardiac deficits offspring. Currently, no studies have explored an intervention for these THC-induced deficits. Omega-3 fatty acids been shown exhibit cardioprotective effects. In this present study, we demonstrated dietary supplementation of omega-3 ameliorates both fetal growth and...
The placenta is a transient organ that develops upon the initiation of pregnancy and essential for embryonic development fetal survival. rodent consists distinct lineages includes cell types are analogous to those make up human placenta. Trophoblast cells within labyrinth layer, which lies closest fetus, fuse come in contact with maternal blood, thus facilitating nutrient waste exchange between mother baby. Abnormalities may occur as result cellular stress have been associated...
Abstract This study characterized the effect of reduced utero-placental perfusion pressure (RUPP) model placental insufficiency on morphology and trophoblast differentiation at mid-late gestation (E14.5). Altered proliferation, syncytiotrophoblast gene expression, increased numbers sinusoidal giant cells, decreased Vegfa pericyte presence in labyrinth were observed addition to changes maternal blood spaces, fetal capillary network weight. Further, junctional zone was by spongiotrophoblast...
The major milestones in mouse placental development are well described, but our understanding is limited to how the placenta can adapt damage or changes environment. By using stereology and expression of cell cycle markers, we found that grows under normal conditions not just by hyperplasia trophoblast cells also through extensive polyploidy hypertrophy. In response feeding a low protein diet mothers prior during pregnancy, mimic chronic malnutrition, this program was altered it influenced...
Studies indicate that ∼7% of pregnant individuals in North America consume cannabis pregnancy. Pre-clinical studies have established maternal exposure to Δ
Placental development is a dynamic and complex process much of our current understanding the underlying molecular processes comes from analysis targeted gene mutations in mice. There are more than 50 strains mutant mice that have placental defects, it has become widely appreciated defects should be suspected cases where embryonic lethality observed. The degree to which these phenotypes investigated highly variable, owing general lack expertise field. However, there been considerable progress...
Background information . The MAPK (mitogen‐activated protein kinase) superfamily of proteins consists four separate signalling cascades: the c‐Jun N‐terminal kinase or stress‐activated kinases (JNK/SAPK); ERKs (extracellular‐signal‐regulated kinases); ERK5 big MAPK1; and p38 group kinases, all which are highly conserved. To date, our studies have focused on defining role pathway during preimplantation development. regulates actin filament formation through downstream MAPKAPK2/3...