A5041850826- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- CRISPR and Genetic Engineering
- Renal and related cancers
- Cancer Research and Treatments
- Tissue Engineering and Regenerative Medicine
- Epigenetics and DNA Methylation
- Urological Disorders and Treatments
- Digestive system and related health
- Cancer, Hypoxia, and Metabolism
- Animal Genetics and Reproduction
- Mitochondrial Function and Pathology
- Monoclonal and Polyclonal Antibodies Research
- 3D Printing in Biomedical Research
- Genomics and Chromatin Dynamics
- Radiopharmaceutical Chemistry and Applications
- Nanoparticle-Based Drug Delivery
- Wnt/β-catenin signaling in development and cancer
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- FOXO transcription factor regulation
- Neurogenesis and neuroplasticity mechanisms
- Glioma Diagnosis and Treatment
- Virus-based gene therapy research
- Algal biology and biofuel production
Institute of Cytology
2013-2024
Russian Academy of Sciences
2010-2015
Max Planck Institute of Immunobiology and Epigenetics
2004-2007
Max Planck Society
2005
Thomas Jefferson University
1998-2003
In mammals, cell lineage specification is established at the blastocyst stage. At this stage, transcription factor Cdx2 represses pluripotency genes, thus promoting extraembryonic trophoblast fate. Recently, Gata3 was shown to act in a parallel pathway fate, suggesting that there are more factors working lineage. Here, we report Tcfap2c expressed high level trophectoderm and able induce fate embryonic stem cells. Trophoblast induced by does not require vice versa, molecules alternative...
Active transcriptional repression has been characterized as a function of many regulatory factors. It facilitates combinatorial regulation gene expression by allowing repressors to be dominant over activators under certain conditions. Here, we show that the Engrailed protein uses two distinct mechanisms repress transcription. One activity is predominant normal transient transfection assay conditions in cultured cells. A second an vivo active assay. The domain mediating (eh1) highly conserved...
Two cDNAs encoding human lysyl-tRNA synthetase have been identified. One encodes the cytoplasmic form of enzyme identified previously. The second cDNA contains same sequence but with a 180-bp insertion at 5′-end mRNA. This results in predicted protein whose carboxyl 576 amino acids are identical to those different terminus 49 that putative mitochondrial targeting sequence. Expression two synthetase-green fluorescent gene fusions cell line confirmed was targeted cytoplasm and mitochondria....
The mitochondrial genomes of Chlamydomonad algae lack the cox2 gene that encodes essential subunit COX II cytochrome c oxidase. is normally a single polypeptide encoded by gene. In this work we cloned two nuclear genes encoding from both Chlamydomonas reinhardtii and Polytomella sp. cox2agene protein, IIA, corresponding to N-terminal portion oxidase, thecox2b IIB, C-terminal region. cox2a cox2b are located in nucleus independently transcribed into mRNAs translated separate polypeptides....
Besides holding great promise in clinics, embryonic stem (ES) cells represent a valuable tool for studying regulation of early developmental processes, such as cell differentiation preimplantation embryos. The caudal-related homeobox protein Cdx2 is transcriptional regulator essential trophoblast lineage, functioning implantation. Using an inducible system, we show that gain function ES triggers trophoblast-like morphological differentiation, accompanied by ploidy increase, onset expression...
Stress-induced premature cell senescence is well recognized to be accompanied by emerging the senescenceassociated secretory phenotype (SASP).Secreted SASP factors can promote of normal neighboring cells through autocrine/paracrine pathways and regulate response, as well.Regarding human endometrium-derived mesenchymal stem (MESCs), regulation mechanisms paracrine activity senescent have not been studied yet.Here, we examined role insulinlike growth factor binding protein 3 (IGFBP3) in...
The mesenchymal stromal/stem cells (MSCs) are known to secrete pleiotropic paracrine factors, contributing tissue regeneration. This unique ability makes MSCs promising therapeutic tools for many diseases, including even those that were previously untreatable. Thus, the development of preconditioning approaches aimed at enhancing function attracts great interest. In present work, we studied how extracellular matrix, essential part native microenvironment, affects secretory capacity various...
The trophectoderm (TE) of blastocysts, the first epithelium established in mammalian development, (1) plays signaling, supportive, and patterning functions during preimplantation (2) ensures embryo implantation into uterine wall, (3) gives rise to extraembryonic tissues essential for growth after implantation. We show that mouse TE, itself permissive lentiviral (LV) infection, represents a robust nonpermeable physical barrier virus particles, thereby shielding cells inner cell mass from...
The algae of the family Chlamydomonadaceae lack gene <i>cox3</i> that encodes subunit III cytochrome <i>c</i> oxidase in their mitochondrial genomes. This observation has raised question whether this is present or corresponding located nucleus. Cytochrome was isolated from colorless chlamydomonad <i>Polytomella</i> spp., and existence established by immunoblotting analysis with an antibody directed against <i>Saccharomyces cerevisiae</i>subunit III. Based partly upon N-terminal sequence...
Engrailed is a key transcriptional regulator in the nervous system and maintenance of developmental boundaries Drosophila, its vertebrate homologs regulate brain limb development. Here, we show that functions both Hox cofactors Extradenticle Homothorax play essential roles repression by Engrailed. Mutations remove either them abrogate ability to repress target genes embryos, interact directly with Engrailed, stimulate cultured cells. We suggest model which function as complex genes. These...
The rat represents an important animal model that, in many respects, is superior to the mouse for dissecting behavioral, cardiovascular and other physiological pathologies relevant humans. Derivation of induced pluripotent stem cells from rats (riPS) opens opportunity gene targeting specific strains, as well development new protocols treatment different degenerative diseases. Here, we report improved lentivirus-based hit-and-run riPS derivation protocol that makes use small inhibitors MEK...
Human artificial chromosomes (HACs) are vectors that offer advantages of capacity and stability for gene delivery expression. Several studies have even demonstrated their use complementation in gene-deficient recipient cell lines animal transgenesis. Recently, we constructed an advance HAC-based vector, alphoidtetO-HAC, with a conditional centromere. In this HAC, gene-loading site was inserted into centrochromatin domain critical kinetochore assembly maintenance. While by definition is...
Despite multimodal approaches for the treatment of multiforme glioblastoma (GBM) advances in outcome have been very modest indicating necessity novel diagnostic and therapeutic strategies. Currently, mesenchymal stem cells (MSCs) represent a promising platform cell-based cancer therapies because their tumor-tropism, low immunogenicity, easy accessibility, isolation procedure, culturing. In present study, we assessed tumor-tropism biodistribution superparamagnetic iron oxide nanoparticle...
Cancer stem cells' (CSCs) self-maintenance is regulated via the pluripotency pathways promoting most aggressive tumor phenotype. This study aimed to use activity of these for CSCs' subpopulation enrichment and separating cells characterized by OCT4 SOX2 expression.To select analyze CSCs, we used SORE6x lentiviral reporter plasmid viral transduction colon adenocarcinoma cells. Additionally, assessed cell chemoresistance, clonogenic, invasive migratory data mRNA-seq intrinsic disorder...
P53 is a critical tumor suppressor that protects the integrity of genome and prevents cells from malignant transformation, including metastases. One driving forces behind onset metastases epithelial to mesenchymal transition (EMT) program. Zeb1 one key transcription factors govern EMT (TF-EMT). Therefore, interaction mutual influence p53 plays role in carcinogenesis. Another important feature tumors their heterogeneity mediated by presence so-called cancer stem (CSCs). To this end, we have...
The principal cause of death in cancer involves tumor progression and metastasis. Since only a small proportion the primary cells, stem cells (CSCs), which are most aggressive, have capacity to metastasize display properties it is imperative characterize gene expression diagnostic markers evaluate drug sensitivity CSCs themselves. Here, we examined key genes that involved colorectal expressed cells. Primary cultures from patient's tumors were studied using flow cytometry cytological methods....