A5039630538- Mitochondrial Function and Pathology
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- ATP Synthase and ATPases Research
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- RNA modifications and cancer
- Microbial Community Ecology and Physiology
- Algal biology and biofuel production
- Protist diversity and phylogeny
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Metabolism and Genetic Disorders
- Marine and coastal ecosystems
- Lymphoma Diagnosis and Treatment
- Lipid metabolism and biosynthesis
- Genetics, Aging, and Longevity in Model Organisms
- Ubiquitin and proteasome pathways
- Bacteriophages and microbial interactions
- Microbial Metabolic Engineering and Bioproduction
- Genomics, phytochemicals, and oxidative stress
- Studies on Chitinases and Chitosanases
- Viral-associated cancers and disorders
- Plant Genetic and Mutation Studies
- Cancer-related gene regulation
Instituto Nacional de Cardiología
2024
Universidad Nacional Autónoma de México
2010-2022
Ludwig-Maximilians-Universität München
2003-2011
University of Kaiserslautern
2010
Center for Integrated Protein Science Munich
2009
Ospedale Martini
2008
The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. Although Sec complexes can form oligomers, it has been suggested that single copy may serve as an active PCC. We determined subnanometer-resolution cryo-electron microscopy structures of eukaryotic ribosome-Sec61 complexes. In combination with biochemical data, we found in both idle states, the not oligomeric interacts mainly via two cytoplasmic loops universal ribosomal adaptor site....
Apicomplexan parasites, including the human pathogens Toxoplasma and Plasmodium , contain a vestigial plastid, apicoplast. This chloroplast-derived organelle is remnant of secondary endosymbiosis between an ancestral apicomplexan photosynthetic organism whose origin moot ([1–4][
YidC/Oxa/Alb3 family proteins catalyze the insertion of integral membrane in bacteria, mitochondria, and chloroplasts, respectively. Unlike gram-negative organisms, gram-positive bacteria express 2 paralogs this family, YidC1/SpoIIIJ YidC2/YgjG. In Streptococcus mutans, deletion yidC2 results a stress-sensitive phenotype similar to that mutants lacking signal recognition particle (SRP) protein translocation pathway, while yidC1 has less severe phenotype. contrast eukaryotes SRP-deficient are...
The mitochondrial genomes of Chlamydomonad algae lack the cox2 gene that encodes essential subunit COX II cytochrome c oxidase. is normally a single polypeptide encoded by gene. In this work we cloned two nuclear genes encoding from both Chlamydomonas reinhardtii and Polytomella sp. cox2agene protein, IIA, corresponding to N-terminal portion oxidase, thecox2b IIB, C-terminal region. cox2a cox2b are located in nucleus independently transcribed into mRNAs translated separate polypeptides....
The atp6 gene, encoding the ATP6 subunit of F(1)F(0)-ATP synthase, has thus far been found only as an mtDNA-encoded gene. However, is absent from mtDNAs some species, including that Chlamydomonas reinhardtii. Analysis C. reinhardtii expressed sequence tags revealed three overlapping sequences encoded a protein with similarity to proteins. PCR and 5'- 3'-RACE were used obtain complete cDNA genomic atp6. gene exhibited characteristics nucleus-encoded gene: Southern hybridization signals...
Proteins of the Oxa1/YidC/Alb3 family mediate insertion proteins into membranes mitochondria, bacteria, and chloroplasts. Here we report identification a second gene in genome Neurospora crassa, which have named oxa2. Its product, Oxa2, is located inner membrane mitochondria. Deletion oxa2 caused specific defect biogenesis cytochrome oxidase resulted induction alternative (AOD), bypasses need for complex IV respiratory chain. The Oxa2 protein N. crassa complements Cox18-deficient yeast...
Biogenesis of respiratory chain complexes depends on the expression mitochondrial-encoded subunits. Their synthesis occurs membrane-associated ribosomes and is probably coupled to their membrane insertion. Defects in mitochondrial translation products are among major causes disorders. Mdm38 related Letm1, a protein affected Wolf-Hirschhorn syndrome patients. Like Mba1 Oxa1, an inner that interacts with involved biogenesis. We find simultaneous loss severe synthetic defects biogenesis...
Recently, the bacterial elongation factor LepA was identified as critical for accuracy of in vitro translation reactions. Extremely well conserved homologues are present throughout bacteria and eukaryotes, but physiological relevance these proteins is unclear. Here we show that yeast counterpart LepA, Guf1, located mitochondrial matrix tightly associated with inner membrane. It binds to ribosomes a GTP-dependent manner. Mutants lacking Guf1 cold- heat-sensitive growth defects on...
The prohibitins Phb1 and Phb2 assemble at the mitochondrial inner membrane to form a multi-dimeric complex. These scaffold proteins are highly conserved in eukaryotic cells, from yeast mammals, have been implicated variety of functions including aging, proliferation, degenerative metabolic diseases. In PHB2 regulates PINK1-PRKN mediated mitophagy by interacting with lipidated MAP1LC3B/LC3B. Despite their high conservation, not linked budding yeasts. this study, we demonstrate that both...
The mitochondrial proteome is mostly composed of nuclear-encoded proteins. Such polypeptides are synthesized with signals that guide their intracellular transport to the surface organelle and later within different subcompartments until they reach functional destination. It has been suggested nascent-polypeptide associated complex (NAC) - a cytosolic chaperone recognizes nascent chains on translationally active ribosomes role in import However, molecular mechanisms regulate NAC-mediated...
The chronological lifespan of Saccharomyces cerevisiae has been pivotal in advancing our understanding aging eukaryotic cells. However, gaining a genome-wide perspective this trait remains challenging due to substantial discrepancies observed across large-scale gene-deletion screens. In study, we performed meta-analysis compile ranked catalog key processes and regulators driving longevity yeast, ensuring their robustness diverse experimental setups. These consistent factors were enriched...
The algae of the family Chlamydomonadaceae lack gene <i>cox3</i> that encodes subunit III cytochrome <i>c</i> oxidase in their mitochondrial genomes. This observation has raised question whether this is present or corresponding located nucleus. Cytochrome was isolated from colorless chlamydomonad <i>Polytomella</i> spp., and existence established by immunoblotting analysis with an antibody directed against <i>Saccharomyces cerevisiae</i>subunit III. Based partly upon N-terminal sequence...
The Arabidopsis thaliana protein ARTEMIS is an integral component of the chloroplast inner envelope required for division. It contains a domain significant homology to members Alb3/Oxa1/YidC family. Here, we show that upon expression in yeast mitochondria, can partially take over function Oxa1 insertion and assembly mitochondrial membrane proteins. This identifies as functional member family suggests existence novel sorting pathway chloroplasts which integrates polypeptides from stroma into...
ABSTRACT The sequencing of the genome Schizosaccharomyces pombe revealed presence a number genes encoding tandem proteins, some which are mitochondrial components. One these proteins (pre-Rsm22-Cox11) consists fusion Rsm22, component ribosome, and Cox11, factor required for copper insertion into cytochrome oxidase. Since in Saccharomyces cerevisiae , Cox11 is physically attached to it was suggested that organization Rsm22-Cox11 used covalently tie ribosome S. . We report here pre-Rsm22-Cox11...