A5057272279- Cancer Cells and Metastasis
- Cancer-related Molecular Pathways
- Prostate Cancer Treatment and Research
- Virus-based gene therapy research
- PI3K/AKT/mTOR signaling in cancer
- Cancer Genomics and Diagnostics
- Radiomics and Machine Learning in Medical Imaging
- HER2/EGFR in Cancer Research
- Animal Genetics and Reproduction
- Cytokine Signaling Pathways and Interactions
- Fibroblast Growth Factor Research
- RNA modifications and cancer
- Wnt/β-catenin signaling in development and cancer
- Monoclonal and Polyclonal Antibodies Research
- Estrogen and related hormone effects
- Cancer-related gene regulation
- Mechanisms of cancer metastasis
- Molecular Biology Techniques and Applications
- Protein Tyrosine Phosphatases
- NF-κB Signaling Pathways
- Kruppel-like factors research
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- 14-3-3 protein interactions
- Cancer Research and Treatments
University of California, Davis
2016-2025
National Center for Infectious Diseases
2025
Weatherford College
2023
City of Hope
2020
National Institutes of Health
2002-2018
National Institute of Environmental Health Sciences
2018
National Cancer Institute
2018
Center for Cancer Research
2018
Cold Spring Harbor Laboratory
2014
Ontario Institute for Cancer Research
2014
The effect of mammary gland-specific expression the polyomavirus middle T antigen was examined by establishing lines transgenic mice that carry oncogene under transcriptional control mouse tumor virus promoter/enhancer. By contrast to most strains carrying activated oncogenes, resulted in widespread transformation epithelium and rapid production multifocal adenocarcinomas. Interestingly, majority tumor-bearing developed secondary metastatic tumors lung. Taken together, these results suggest...
Overexpression and amplification of the neu (c-erbB2, ERBB2) protooncogene have been implicated in development aggressive human breast cancer. To directly assess effect mammary gland-specific expression protooncogene, transgenic mice carrying unactivated under transcriptional control mouse tumor virus promoter/enhancer were established. By contrast to rapid progression observed several strains activated transgene, epithelium resulted focal tumors after long latency. The majority analyzed...
The effect of mammary gland-specific expression the polyomavirus middle T antigen was examined by establishing lines transgenic mice that carry oncogene under transcriptional control mouse tumor virus promoter/enhancer. By contrast to most strains carrying activated oncogenes, resulted in widespread transformation epithelium and rapid production multifocal adenocarcinomas. Interestingly, majority tumor-bearing developed secondary metastatic tumors lung. Taken together, these results suggest...
The hematoxylin and eosin (H&E) stain is the standard used for microscopic examination of tissues that have been fixed, processed, embedded, sectioned. It can be performed manually or by automation. For economic reasons, manual technique generally method choice facilities with a low sample volume. This protocol describes H&E staining paraffin-embedded, sectioned mouse tissues. In H&E-stained tissues, nucleic acids dark blue proteins red to pink orange. accurate phenotyping delineation tissue...
In aging men, the prostate gland becomes hyperproliferative and displays a propensity toward carcinoma. Although this process has been proposed to represent an inappropriate reactivation of embryonic differentiation program, regulatory genes responsible for normal development function are largely undefined. Here we show that murine Nkx3.1 homeobox gene is earliest known marker epithelium during embryogenesis subsequently expressed at all stages in vivo as well tissue recombinants. A null...
The influence of transforming growth factor β (TGF-β) signaling on Neu-induced mammary tumorigenesis and metastasis was examined with transgenic mouse models. We generated mice expressing an activated TGF-β type I receptor or dominant negative II under control the tumor virus promoter. When crossed forms Neu tyrosine kinase that selectively couple to Grb2 Shc pathways increased latency formation but also enhanced frequency extravascular lung metastasis. Conversely, expression decreased while...
Animal models, particularly mouse play a central role in the study of etiology, prevention, and treatment human prostate cancer. While tissue culture models are extremely useful understanding biology cancer, they cannot recapitulate complex cellular interactions within tumor microenvironment that key cancer initiation progression. The National Cancer Institute (NCI) Mouse Models Human Cancers Consortium convened group veterinary pathologists to review current animal make recommendations...
ABSTRACT Cancer screening relies upon a linear model of neoplastic growth and progression. Yet, historical observations suggest that malignant progression is uncoupled from growth, which may explain the paradoxical increase in early-stage breast cancer detection without dramatic reduction metastasis. Here, we lineage trace millions transformed cells thousands tumors using rainbow mouse HER2 (also known as ERBB2)-positive cancer. Transition rates field cell to screen-detectable tumor...
Mouse models have provided significant insights into the molecular mechanisms of tumor suppressor gene function. Here we use mouse prostate carcinogenesis to demonstrate that Nkx3.1 homeobox undergoes epigenetic inactivation through loss protein expression. Loss function in mice cooperates with Pten cancer progression. This cooperativity results synergistic activation Akt (protein kinase B), a key modulator cell growth and survival. Our findings underscore significance interactions between...