A5044756037- PI3K/AKT/mTOR signaling in cancer
- Prostate Cancer Treatment and Research
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Acute Lymphoblastic Leukemia research
- Cancer-related molecular mechanisms research
- Cell Adhesion Molecules Research
- Chronic Myeloid Leukemia Treatments
- Cancer, Hypoxia, and Metabolism
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cytokine Signaling Pathways and Interactions
- Protein Kinase Regulation and GTPase Signaling
- Cancer-related gene regulation
- Cancer Genomics and Diagnostics
- Pluripotent Stem Cells Research
- Cancer Mechanisms and Therapy
- Renal and related cancers
- Pancreatic and Hepatic Oncology Research
- Autophagy in Disease and Therapy
- interferon and immune responses
- Sarcoma Diagnosis and Treatment
- Hedgehog Signaling Pathway Studies
- Pancreatic function and diabetes
University of Alberta
2013-2025
Peking University
2015-2024
Center for Life Sciences
2014-2024
Zhejiang University
2017-2024
Wuxi People's Hospital
2024
Tibet Autonomous Region People's Hospital
2024
Wuhan University of Science and Technology
2024
Fujian Medical University
2024
First Affiliated Hospital Zhejiang University
2021-2023
King Center
2018-2022
Recent evidence places the FRAP/mTOR kinase downstream of phosphatidyl inositol 3-kinase/Akt-signaling pathway, which is up-regulated in multiple cancers because loss PTEN tumor suppressor gene. We performed biological and biochemical studies to determine whether PTEN-deficient cancer cells are sensitive pharmacologic inhibition by using rapamycin derivative CCI-779. In vitro vivo isogenic +/+ −/− mouse as well human with defined status showed that growth null was blocked preferentially...
To investigate the molecular basis of PTEN-mediated tumor suppression, we introduced a null mutation into mouse Pten gene by homologous recombination in embryonic stem (ES) cells. Pten-/- ES cells exhibited an increased growth rate and proliferated even absence serum. lacking PTEN function also displayed advanced entry S phase. This accelerated G1/S transition was accompanied down-regulation p27(KIP1), major inhibitor for G1 cyclin-dependent kinases. Inactivation fibroblasts resulted...
The mechanisms controlling neural stem cell proliferation are poorly understood. Here we demonstrate that the PTEN tumor suppressor plays an important role in regulating stem/progenitor cells vivo and vitro. Mice lacking exhibited enlarged, histoarchitecturally abnormal brains, which resulted from increased proliferation, decreased death, enlarged size. Neurosphere cultures revealed a greater capacity for tripotent Pten-/- central nervous system cells, can be attributed, at least part, to...
Type III collagen is a fibrillar forming comprising three α1(III) chains and expressed in early embryos throughout embryogenesis. In the adult, type major component of extracellular matrix variety internal organs skin. Mutations COL3A1 gene have been implicated as cause IV Ehlers–Danlos syndrome, disease leading to aortic rupture adult life. To directly study role Col3a1 development disease, we inactivated embryonic stem cells by homologous recombination. The mutated allele was transmitted...
A grabby substrate: 3D nanostructured substrate, namely, a silicon-nanopillar (SiNP) array coated with epithelial-cell adhesion-molecule antibody (anti-EpCAM), shows enhanced local topographic interactions between nanoscale cell-surface components and the substrates surface, resulting in cell-capture efficiency when employed to isolate viable cancer cells from whole-blood samples (see schematic SEM image of captured cell).
PTEN loss or PI3K/AKT signaling pathway activation correlates with human prostate cancer progression and metastasis. However, in preclinical murine models, deletion of Pten alone fails to mimic the significant metastatic burden that frequently accompanies end stage disease. To identify additional alterations cooperate progression, we surveyed tissue microarrays found RAS/MAPK is significantly elevated both primary lesions. In an attempt model this event, crossed conditional activatable...
In the liver, insulin controls both lipid and glucose metabolism through its cell surface receptor intracellular mediators such as phosphatidylinositol 3-kinase serine-threonine kinase AKT. The signaling pathway is further modulated by protein tyrosine phosphatase or phosphatase. Here, we investigated function of tension homologue deleted on chromosome 10 (PTEN), a negative regulator 3-kinase/AKT pathway, targeted deletion Pten in murine liver. Deletion liver resulted increased fatty acid...
Erythropoietin (EPO) is the principal growth factor regulating production of red blood cells. Recent studies demonstrated that exogenous EPO acts as a neuroprotectant and regulates neurogenesis. Using genetic approach, we evaluate roles endogenous its classical receptor (EPOR) in mammalian We demonstrate severe identical embryonic neurogenesis defects animals null for either Epo or EpoR gene, suggesting EPOR essential action during Furthermore, by generating conditional knock-down animals,...
Although Akt is known as a survival kinase, inhibitors of the phosphatidylinositol 3-kinase (PI3K)–Akt pathway do not always induce substantial apoptosis. We show that silencing Akt1 alone, or any combination isoforms, can suppress growth tumors established from phosphatase and tensin homologue–null human cancer cells. these findings indicate essential for tumor maintenance, most eventually rebound. knockdown inactivation with small molecule did significant apoptosis but rather markedly...
Oncosomes are tumor-derived microvesicles that transmit signaling complexes between cell and tissue compartments. Herein, we show amoeboid tumor cells export large (1- to 10-μm diameter) vesicles, derived from bulky cellular protrusions, contain metalloproteinases, RNA, caveolin-1, the GTPase ADP-ribosylation factor 6, biologically active toward cells, endothelial fibroblasts. We describe methods by which oncosomes can be selectively sorted flow cytometry analyzed independently of vesicles...
Quercetin, a dietary antioxidant present in fruits and vegetables, is promising cancer chemopreventive agent that inhibits tumor promotion by inducing cell cycle arrest promoting apoptotic death. In this study, we examined the biological activities of quercetin against gastric cancer. Our studies demonstrated exposure cells AGS MKN28 to resulted pronounced pro-apoptotic effect through activating mitochondria pathway. Meanwhile, treatment with induced appearance autophagic vacuoles, formation...