A5100769312- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Acute Myeloid Leukemia Research
- Chromium effects and bioremediation
- Protein Tyrosine Phosphatases
- Cancer-related Molecular Pathways
- RNA Research and Splicing
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related molecular mechanisms research
- Heavy Metal Exposure and Toxicity
- Circular RNAs in diseases
- Cell Adhesion Molecules Research
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Retinoids in leukemia and cellular processes
- Protein Degradation and Inhibitors
- Histone Deacetylase Inhibitors Research
- Trace Elements in Health
- Protein Kinase Regulation and GTPase Signaling
- Organ Transplantation Techniques and Outcomes
- Arsenic contamination and mitigation
New York University
2014-2024
University of Nevada, Las Vegas
2012-2024
Second Affiliated Hospital of Xi'an Jiaotong University
2015-2024
Yidu Central Hospital of Weifang
2018-2020
Digestive Health Research Institute
2020
Henan University
2020
Shihezi University
2020
Wuhan Children's Hospital
2018-2019
Huazhong University of Science and Technology
2018-2019
Central South University
2009-2017
To investigate the molecular basis of PTEN-mediated tumor suppression, we introduced a null mutation into mouse Pten gene by homologous recombination in embryonic stem (ES) cells. Pten-/- ES cells exhibited an increased growth rate and proliferated even absence serum. lacking PTEN function also displayed advanced entry S phase. This accelerated G1/S transition was accompanied down-regulation p27(KIP1), major inhibitor for G1 cyclin-dependent kinases. Inactivation fibroblasts resulted...
PTEN/MMAC1/TEP1 is a tumor suppressor that possesses intrinsic phosphatase activity. Deletions or mutations of its encoding gene are associated with variety human cancers. However, very little known about the molecular mechanisms by which this important regulates cell growth. Here, we show PTEN expression potently suppressed growth and tumorigenicity glioblastoma U87MG cells. The suppression activity was mediated ability to block cycle progression in G 1 phase. Such an arrest correlated...
Abstract Mutations in the tumor suppressor gene PTEN (MMAC1/TEP1) are associated with a large number of human cancers and several autosomal-dominant disorders. Mice mutant for die at early embryonic stages fibroblasts display decreased sensitivity to cell death. Overexpression different mammalian tissue culture cells affects various processes including proliferation, death migration. We have characterized Drosophila present evidence that both inactivation overexpression affect size, while...
Mitogen-activated protein kinases (MAP kinases) are common components of signaling pathways induced by diverse growth stimuli. Although the guanidine nucleotide-binding Ras proteins known to be upstream activators MAP kinases, extent which directly contribute mitogenic effect is as yet undefined. In this study, inhibition kinase phosphatase MKP-1 blocked induction DNA synthesis in quiescent rat embryonic fibroblast REF-52 cells an activated mutant Ras, V 12 Ras. These results suggest...
Histone modification determines epigenetic patterns of gene expression with methylation histone H3 at lysine 4 (H3K4) often associated active promoters. LSD1/KDM1 is a demethylase that suppresses by converting dimethylated H3K4 to mono- and unmethylated H3K4. LSD1 essential for metazoan development, but its pathophysiologic functions in cancer remain mainly uncharacterized. In this study, we developed specific bioactive small inhibitors enhance derepress epigenetically suppressed genes vivo....
Stimulation of fibroblasts with serum growth factors results in the rapid activation a set immediate-early genes, among them 3CH134. We have purified bacterially expressed form 3CH134-encoded polypeptide and demonstrated that it has intrinsic protein-tyrosine-phosphatase (PTPase; protein-tyrosine-phosphate phosphohydrolase, EC 3.1.3.48) activity vitro. This is optimal at pH 7.5, sensitive to vanadate cysteinyl modifying agents, insensitive panel serine/threonine phosphatase inhibitors....
The Saccharomyces cerevisiae Sgs1p helicase localizes to the nucleolus and is required maintain integrity of rDNA repeats. a member RecQ DNA family, which also includes Schizosaccharomyces pombe Rqh1, human BLM WRN genes. These genes encode proteins are essential maintenance genomic share highly conserved domain. Here we show that recombinant efficiently unwinds guanine-guanine (G-G) paired DNA. Unwinding G-G ATP- Mg2+-dependent requires short 3′ single-stranded tail. Strikingly, substrates...
Histone H3 lysine 4 (H3K4) trimethylation (H3K4me3) at the promoter region of genes has been linked to transcriptional activation. In present study, we found that hypoxia (1% oxygen) increased H3K4me3 in both normal human bronchial epithelial Beas-2B cells and lung carcinoma A549 cells. The increase from was likely caused by inhibition H3K4 demethylating activity, as still methionine-deficient medium. Furthermore, an vitro histone demethylation assay showed 1% oxygen decreased activity...
Occupational exposure to nickel (Ni) is associated with an increased risk for lung and nasal cancers. Ni compounds exhibit weak mutagenic activity, cause gene amplification, disrupt cellular epigenetic homeostasis. However, the Ni-induced changes in global histone modification levels have only been tested vitro.This study was conducted a Chinese population determine whether occupational alterations of evaluate inter- intraindividual variance levels.Forty-five subjects 75 referents were...
Abstract Background: Exposure to arsenic (As) is associated with an increased risk of several cancers as well cardiovascular disease, and childhood neuro-developmental deficits. Arsenic compounds are weakly mutagenic, alter gene expression posttranslational histone modifications (PTHMs) in vitro. Methods: Water urinary As concentrations global levels 3 lysine 9 di-methylation acetylation (H3K9me2 H3K9ac), 27 tri-methylation (H3K27me3 H3K27ac), 18 (H3K18ac), 4 trimethylation (H3K4me3) were...
Gene amplification of Sox2 at 3q26.33 is a common event in squamous cell carcinomas (SCCs) the lung and esophagus, as well several other cancers. Here, we show that expression LSD1/KDM1 histone demethylase significantly elevated Sox2-expressing SCCs. LSD1-specific inhibitors selectively impair growth SCCs, but not Sox2-negative cells. associated with sensitivity to LSD1 inhibition lung, breast, ovarian, carcinoma Inactivation reduces expression, promotes G1 cell-cycle arrest, induces genes...