A5100326016- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Bacterial Genetics and Biotechnology
- Ubiquitin and proteasome pathways
- Bacteriophages and microbial interactions
- Cancer Research and Treatments
- SARS-CoV-2 and COVID-19 Research
- Genomics and Phylogenetic Studies
- Oxidative Organic Chemistry Reactions
- Cancer-related Molecular Pathways
- Genetic diversity and population structure
- Enzyme Structure and Function
- Ferroptosis and cancer prognosis
- Synthesis and Catalytic Reactions
- Catalytic C–H Functionalization Methods
- MicroRNA in disease regulation
- Identification and Quantification in Food
- Tryptophan and brain disorders
- Pharmacological Receptor Mechanisms and Effects
- Nanoplatforms for cancer theranostics
- Genetic Mapping and Diversity in Plants and Animals
- PI3K/AKT/mTOR signaling in cancer
- Synthetic Organic Chemistry Methods
- Virus-based gene therapy research
- Heat shock proteins research
UConn Health
2015-2025
Guangdong Laboratory Animals Monitoring Institute
2020-2025
University of Connecticut
2010-2025
Beijing Institute of Education
2022-2024
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2022-2024
Inner Mongolia People's Hospital
2024
Hunan Normal University
2024
Hunan Provincial People's Hospital
2024
Kunming Medical University
2013-2023
Guangdong Medical College
2023
The outbreak of coronavirus disease 2019 (COVID-19) has resulted in a global pandemic due to the rapid spread severe acute respiratory syndrome 2 (SARS-CoV-2). At time this manuscript's publication, remdesivir is only COVID-19 treatment approved by United States Food and Drug Administration. However, its effectiveness still under question results large Solidarity Trial conducted World Health Organization. Herein, we report that parent nucleoside remdesivir, GS-441524, potently inhibits...
To carry out an integrative profile of human pancreatic ductal adenocarcinoma (PDAC) to identify prognosis-significant genes and their related pathways.
In the ubiquitin proteasome system, E3 ligase SCF-Skp2 and its accessory protein, Cks1, promote proliferation largely by inducing degradation of CDK inhibitor p27. Overexpression Skp2 in human cancers correlates with poor prognosis, deregulation SCF-Skp2-Cks1 promotes tumorigenesis animal models. We identified small molecule inhibitors specific to activity using silico screens targeted binding interface for These compounds selectively inhibited Skp2-mediated p27 reducing through key...
Cytosolic HSP90 requires multiple cochaperones in folding client proteins. However, the function of gp96 (HSP90b1, grp94), an paralogue endoplasmic reticulum (ER), is believed to be independent cochaperones. Here, we demonstrate that chaperones Toll-like receptors (TLRs), but not TLR3, a manner dependent on another ER luminal protein, CNPY3. directly interacts with CNPY3, and complex dissociates presence adenosine triphosphate (ATP). Genetic disruption gp96–CNPY3 interaction completely...
Epidermal growth factor-like domain 7 (Egfl7) is a recently identified secreted protein that believed to be primarily expressed in endothelial cells (ECs). Although its expression was reported elevated during tumorigenesis, whether and how Egfl7 contributes human malignancies remains unknown. In the present study overexpression of found predominantly hepatocellular carcinoma (HCC) HCC tissues closely correlated with poor prognosis HCC. The cancer further verified cell lines including HepG2,...
Postpartum depression (PPD) is a significant contributor to maternal morbidity and mortality. The Sigma-1 (σ-1) receptor has received increasing attention in recent years because of its ability link different signaling systems exert function the brain through chaperone actions, especially neuropsychiatric disorders. YL-0919, novel σ-1 agonist developed by our institute, shown antidepressive anxiolytic effects variety animal models, but on PPD have not been revealed. In present study,...
// Kei Kawaguchi 1, 2, 3 , Kentaro Igarashi 2 Takashi Murakami Bartosz Chmielowski 4 Tasuku Kiyuna Ming Zhao 1 Yong Zhang Arun Singh Michiaki Unno Scott D. Nelson 5 Tara A. Russell 6 Sarah M. Dry Yunfeng Li Fritz C. Eilber Robert Hoffman AntiCancer, Inc., San Diego, CA, USA Department of Surgery, University California, Graduate School Medicine, Tohoku University, Sendai, Japan Division Hematology-Oncology, Los Angeles, Pathology, Surgical Oncology, Correspondence to: Hoffman, email:...
ABSTRACT The proteins encoded by the spoVA operon, including SpoVAD, are essential for uptake of 1:1 chelate pyridine-2,6-dicarboxylic acid (DPA 2,6 ) and Ca 2+ into developing spores bacterium Bacillus subtilis . crystal structure B. SpoVAD has been determined recently, a structural homology search revealed that shares significant similarity but not sequence to group enzymes bind and/or act on small aromatic molecules. We find molecular docking placed DPA exclusively in highly conserved...
2Duf, named after the presence of a transmembrane (TM) Duf421 domain and small Duf1657 in its sequence, is likely located inner membrane (IM) spores some Bacillus species carrying transposon with an operon termed spoVA 2mob . These are known for their extreme resistance to wet heat, 2Duf believed be primary contributor this trait. In study, we found that absence YetF or YdfS, both domain-containing proteins only wild-type (wt) B. subtilis more abundant, leads decreased heat agents can damage...
Abstract To explore the relationship between miR-373 and occurrence development of colorectal cancer. Additionally, it aims to predict potential cellular signaling pathways regulatory mechanisms in which may be involved provides a theoretical basis experimental evidence for clinical application as biomarker, molecular target, prognostic indicator Real-time quantitative PCR is used analyze expression human cancer cell lines normal colonic epithelial cells. Further validation differential...
N-methyl-D-aspartate receptor (NMDAR)-mediated glutamate excitotoxicity significantly contributes to ischemic neuronal death and post-recanalization infarction expansion. Despite tremendous efforts, targeting NMDARs has proven unsuccessful in clinical trials for mitigating brain injury. Here, we show the discovery of an interaction motif transient potential melastatin 2 (TRPM2) protein kinase Cγ (PKCγ) association demonstrate that TRPM2-PKCγ uncoupling is effective therapeutic strategy...
Synthesize while the iron is hot: The synthesis of 2-vinylquinolines via a FeCl3-catalyzed CH sp3 functionalization and subsequent CN cleavage reported. method simple efficient, avoids use organometallic compounds. A variety were obtained from readily available 2-methylquinolines with moderate to good yields. As service our authors readers, this journal provides supporting information supplied by authors. Such materials are peer reviewed may be re-organized for online delivery, but not...
The translesion synthesis (TLS) polymerases Polζ and Rev1 form a complex that enables replication of damaged DNA. Rev7 subunit Polζ, which is multifaceted HORMA (Hop1, Rev7, Mad2) protein with roles in TLS, DNA repair, cell-cycle control, facilitates assembly this by binding the catalytic Rev3. interacts Rev3 mechanism conserved among proteins, whereby an open-to-closed transition locks ligand underneath "safety belt" loop. Dimerization proteins promotes release ligand, as exemplified...
// Takashi Murakami 1, 2, 3 , Kentaro Igarashi 1 Kei Kawaguchi Tasuku Kiyuna Yong Zhang Ming Zhao Yukihiko Hiroshima Scott D. Nelson 5 Sarah M. Dry Yunfeng Li Jane Yanagawa 6 Tara Russell Noah Federman 7 Arun Singh 4 Irmina Elliott Ryusei Matsuyama Chishima Kuniya Tanaka Itaru Endo Fritz C. Eilber Robert Hoffman 2 AntiCancer, Inc., San Diego, California, USA Department of Surgery, University Gastroenterological Graduate School Medicine, Yokohama City University, Yokohama, Japan Division...
Previously, a BRAF-V600E-mutant melanoma obtained from the right chest wall of patient was grown orthotopically in nude mice to establish patient-derived orthotopic xenograft (PDOX) model. Trametinib (TRA), an MEK inhibitor, caused tumor regression. In contrast, another cobimetinib (COB) could slow but not arrest growth or cause regression PDOX. First-line therapy temozolomide (TEM) addition, vemurafenib (VEM) effective even though VEM is supposed target BRAF-V600E mutation. We also...
Abstract Replication of the coronavirus genome starts with formation viral RNA-containing double-membrane vesicles (DMV) following entry into host cell. The multi-domain nonstructural protein 3 (nsp3) is largest encoded by known and serves as a central component replication transcription machinery. Previous studies demonstrated that highly-conserved C-terminal region nsp3 essential for subcellular membrane rearrangement, yet underlying mechanisms remain elusive. Here we report crystal...
Spondyloarthritis is a prevalent and persistent condition that significantly impacts the quality of life. Its intricate pathological mechanisms have led to scarcity animal models capable replicating disease progression in humans, making it prominent area research interest field. To delve into physiological traits spontaneous non-human primate spondyloarthritis, this study meticulously examined features natural model through an array techniques including X-ray imaging, MRI blood biochemistry,...
Abstract Endothelial cells (ECs) help maintain the blood–brain barrier but deteriorate in many neurodegenerative disorders. Here we show, using a specialized method to isolate EC and microglial nuclei from postmortem human cortex (92 donors, 50 male 42 female, aged 20–98 years), that intranuclear cellular indexing of transcriptomes epitopes enables simultaneous profiling nuclear proteins RNA transcripts at single-nucleus resolution. We identify disease-associated subset capillary ECs...
The Gram-positive, anaerobic, spore-forming bacterium Clostridium perfringens causes a variety of diseases in both humans and animals, spore germination is thought to be the first stage C. infection. Previous studies have indicated that germinant receptor (GR) proteins encoded by bicistronic gerKA-gerKC operon as well gerKB gerAA genes are required for normal spores. We now report individual role these GR analyzing strains carrying mutations gerKA, gerKC, or gerAA. Western blot analysis was...