A5063282437- Prostate Cancer Treatment and Research
- Glutathione Transferases and Polymorphisms
- Cardiac Imaging and Diagnostics
- Extracellular vesicles in disease
- DNA Repair Mechanisms
- Caveolin-1 and cellular processes
- Cardiovascular Function and Risk Factors
- Acute Myocardial Infarction Research
- MicroRNA in disease regulation
- Cardiac Structural Anomalies and Repair
- Atherosclerosis and Cardiovascular Diseases
- Aortic Disease and Treatment Approaches
- Cancer, Lipids, and Metabolism
- Circular RNAs in diseases
- Viral Infections and Immunology Research
- Aortic aneurysm repair treatments
- Coronary Interventions and Diagnostics
- Cardiac Valve Diseases and Treatments
- Pulmonary Hypertension Research and Treatments
- Mechanical Circulatory Support Devices
- Estrogen and related hormone effects
- Cardiovascular Disease and Adiposity
- Ubiquitin and proteasome pathways
- Cardiac tumors and thrombi
- Heat shock proteins research
University of Virginia
2024-2025
University of Brescia
2022-2024
University of Insubria
2021-2023
Boston Children's Hospital
2009-2023
Ospedale di Circolo e Fondazione Macchi
2022-2023
Cedars-Sinai Medical Center
2012-2021
Samueli Institute
2013
Harvard University
2012-2013
University of Florence
2008-2011
Oncosomes have recently been described as membrane-derived microvesicles secreted by cancer cells, which transfer oncogenic signals and protein complexes across cell boundaries. Here, we show the rapid formation secretion of oncosomes from DU145 LNCaP human prostate cells. Oncosome was stimulated epidermal growth factor receptor activation also overexpression membrane-targeted Akt1. Microvesicles shed cells contained numerous signal transduction proteins were capable activating...
Oncosomes are tumor-derived microvesicles that transmit signaling complexes between cell and tissue compartments. Herein, we show amoeboid tumor cells export large (1- to 10-μm diameter) vesicles, derived from bulky cellular protrusions, contain metalloproteinases, RNA, caveolin-1, the GTPase ADP-ribosylation factor 6, biologically active toward cells, endothelial fibroblasts. We describe methods by which oncosomes can be selectively sorted flow cytometry analyzed independently of vesicles...
// Valentina R. Minciacchi 1 , Sungyong You Cristiana Spinelli Samantha Morley 2 Mandana Zandian Paul-Joseph Aspuria 3 Lorenzo Cavallini 1,4 Chiara Ciardiello 1,5 Mariana Reis Sobreiro Matteo Morello Geetanjali Kharmate 6 Su Chul Jang 7 Dae-Kyum Kim Elham Hosseini-Beheshti Emma Tomlinson Guns Martin Gleave Yong Song Gho Suresh Mathivanan 8 Wei Yang Michael Freeman 1,2 and Dolores Di Vizio Division of Cancer Biology Therapeutics, Departments Surgery, Biomedical Sciences Pathology Laboratory...
Prostate cancer cells release atypically large extracellular vesicles (EVs), termed oncosomes, which may play a role in the tumor microenvironment by transporting bioactive molecules across tissue spaces and through blood stream. In this study, we applied novel method for selective isolation of oncosomes applicable to human platelet-poor plasma, where presence caveolin-1-positive identified patients with metastatic disease. This procedure was also used validate results miRNA array performed...
Here, we examined the status of stromal Cav-1 expression in patients with benign prostatic hypertrophy (BPH), primary prostate cancers (PCa), and prostate-cancer metastases (Mets). Interestingly, an absence directly correlated cancer disease progression. For example, virtually all BPH samples showed abundant immunostaining. In contrast, a subset cancer, levels were significantly decreased, this high Gleason score, indicative worse prognosis poor clinical outcome. Remarkably, metastatic...
Levels of caveolin-1 (Cav-1) in tumour epithelial cells increase during prostate cancer progression. Conversely, Cav-1 expression the stroma can decline advanced and metastatic cancer. In a large cohort 724 cancers, we observed significantly decreased levels stromal concordance with increased Gleason score (p = 0.012). Importantly, reduced correlated relapse-free survival 0.009), suggesting role for inhibiting disease. Silencing by shRNA WPMY-1 fibroblasts resulted up-regulation Akt...
Abstract Therapies for most malignancies are generally ineffective once metastasis occurs. While tumour cells migrate through tissues using diverse strategies, the signalling networks controlling such behaviours in human tumours poorly understood. Here we define a role Diaphanous‐related formin‐3 (DIAPH3) as non‐canonical regulator of that restrains conversion to amoeboid cell behaviour multiple cancer types. The DIAPH3 locus is close RB1 , within narrow consensus region deletion on...
Tumour cells release diverse populations of extracellular vesicles (EVs) ranging in size, molecular cargo, and function. We sought to characterize mRNA protein content EV subpopulations released by human glioblastoma (GBM) expressing a mutant form epidermal growth factor receptor (U87
Abstract The role of brain cholesterol in Alzheimer's disease (AD) is currently a matter debate. Experimental evidence suggests that reducing circulating and protects against AD, however recent data indicate low membrane results neurode‐generation the synthesis catalyst seladin‐1 down‐regulated AD‐affected regions. We previously reported significant correlation between resistance to amyloid toxicity content differing cultured cell types. Here we provide Aβ42 pre‐fibrillar aggregates...
Estrogen exerts neuroprotective effects and reduces beta-amyloid accumulation in models of Alzheimer's disease (AD). A few years ago, a new gene, i.e. seladin-1 (for selective AD indicator-1), was identified found to be down-regulated vulnerable brain regions. Seladin-1 inhibits the activation caspase-3, key modulator apoptosis. In addition, it has been demonstrated that gene encodes 3beta-hydroxysterol Delta24-reductase, which catalyzes synthesis cholesterol from desmosterol. We have...
// Murali Gururajan 1, * , Karen A. Cavassani Margarit Sievert 1 Peng Duan Jake Lichterman Jen-Ming Huang Bethany Smith Sungyong You 2 Srinivas Nandana Gina Chia-Yi Chu Sheldon Mink Sajni Josson Chunyan Liu Matteo Morello 3 Lawrence W. M. Jones 4 Jayoung Kim Michael R. Freeman Neil Bhowmick Haiyen E. Zhau Leland W.K. Chung Edwin Posadas Urologic Oncology Program/Uro-Oncology Research Laboratories, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA...
The androgen receptor (AR) is a transcription factor that employs many diverse interactions with coregulatory proteins in normal physiology and prostate cancer (PCa). AR mediates cellular responses association chromatin complexes kinase cascades. Here we report the nuclear matrix protein, scaffold attachment B1 (SAFB1), regulates activity levels manner suggests its involvement PCa. SAFB1 mRNA expression was lower PCa comparison tissue majority of publicly available RNA data sets. protein...
Copy Number Alterations (CNAs) represent the most common genetic alterations identified in ovarian cancer cells, being responsible for extensive genomic instability observed this cancer. Here we report identification of CNAs a cohort Italian patients affected by performed SNP-based array. Our analysis allowed 201 significantly altered chromosomal bands (70 copy number gains; 131 losses). The 3300 genes subjected to CNA here were compared those present TCGA dataset. 11 with increased CN and...
Background. Nowadays, it is still not possible to clinically distinguish whether an increase in high-sensitivity cardiac troponin (hs-cTn) values due myocardial injury or acute coronary syndrome (ACS). Moreover, predictive data regarding hs-cTnT emergency room (ER) setting are scarce. This monocentric retrospective study aimed improve the knowledge and interpretation of this biomarker daily clinical practice. Methods. Consecutive adult patients presenting at ER hospitalized with a first...
To investigate the presence of silicone oil (SO)-emulsification on anterior iris surface with segment optical coherence tomography (AS-OCT).
Patients with ST-segment elevation myocardial infarction (STEMI) and complex coronary artery disease (CAD) face a poor prognosis, including increased heart failure (HF) risk. Phase 2 clinical trials of anakinra have shown inhibition the acute inflammatory response prevention HF after STEMI, but data on its effects based CAD complexity are lacking. We performed pooled secondary analysis 139 patients STEMI. The SYNTAX (Synergy Between Percutaneous Coronary Intervention Taxus Cardiac Surgery),...
Background: Patients with ST-segment elevation myocardial infarction (STEMI) and complex coronary artery disease (CAD) face a poor prognosis, including increased heart failure (HF) risk. Interleukin-1 (IL-1) blockade Anakinra inhibits the acute inflammatory response prevent HF after STEMI, but data on its effects based CAD complexity are lacking. Aims: To assess of in patients across spectrum complexity. Methods: We performed pooled secondary analysis 139 STEMI from three randomized clinical...