A5056961111- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Circular RNAs in diseases
- Diabetes and associated disorders
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Adipokines, Inflammation, and Metabolic Diseases
- TGF-β signaling in diseases
- Pancreatic function and diabetes
- Dietary Effects on Health
- COVID-19 Clinical Research Studies
- Cancer, Hypoxia, and Metabolism
- Cardiovascular Disease and Adiposity
- Immune cells in cancer
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Alzheimer's disease research and treatments
- Genetics, Aging, and Longevity in Model Organisms
- interferon and immune responses
- Diabetes Treatment and Management
- Molecular Biology Techniques and Applications
- Diabetes Management and Research
- Regulation of Appetite and Obesity
- Atherosclerosis and Cardiovascular Diseases
- Bioinformatics and Genomic Networks
University of Naples Federico II
1997-2024
MultiMedica
2016-2021
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2021
Istituto Nazionale Genetica Molecolare
2013-2016
Mylan (Switzerland)
2016
Science for Life Laboratory
2015
Karolinska Institutet
2015
University of Milano-Bicocca
2011
University of Chicago
2008-2009
Memorial Sloan Kettering Cancer Center
2003-2008
ABSTRACT The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions extracellular vesicles (EVs), collective term covering various subtypes cell‐released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, many other names. However, specific issues arise when working with these entities, whose size amount often make them difficult to obtain as relatively...
The release of RNA-containing extracellular vesicles (EV) into the milieu has been demonstrated in a multitude different vitro cell systems and variety body fluids. EV are limelight for their capacity to communicate genetically encoded messages other cells, suitability as candidate biomarkers diseases, use therapeutic agents. Although EV-RNA attracted enormous interest from basic researchers, clinicians, industry, we currently have limited knowledge on which mechanisms drive regulate RNA...
The high-throughput analysis of microRNAs (miRNAs) circulating within the blood healthy and diseased individuals is an active area biomarker research. Whereas quantitative real-time reverse transcription polymerase chain reaction (qPCR)-based methods are widely used, it yet unresolved how data should be normalized. Here, we show that a combination different algorithms results in identification candidate reference miRNAs can exploited as normalizers, both discovery validation phases. Using...
Prostate cancer cells release atypically large extracellular vesicles (EVs), termed oncosomes, which may play a role in the tumor microenvironment by transporting bioactive molecules across tissue spaces and through blood stream. In this study, we applied novel method for selective isolation of oncosomes applicable to human platelet-poor plasma, where presence caveolin-1-positive identified patients with metastatic disease. This procedure was also used validate results miRNA array performed...
Sodium-glucose cotransporter 2 (SGLT-2) inhibitors (i) reduce cardiovascular and renal events in patients with without type diabetes (T2D). However, the underlying mechanisms are debated. Low-grade inflammation (LGI) is a key driver of vascular complications, suggested to be attenuated by SGLT-2i animal models. Based on specific working hypothesis, here we investigated net effect LGI T2D possible mechanism. We enrolled treated either stable therapy or other glucose-lowering drugs (GLD) (n =...
The establishment of distant metastases depends on the capacity small numbers cancer cells to regenerate a tumor after entering target tissue. mechanisms that confer this remain be defined. Here we identify role for transcriptional inhibitors differentiation Id1 and Id3 as selective mediators lung metastatic colonization in triple negative [TN, i.e., lacking expression estrogen receptor progesterone receptor, Her2 (human epidermal growth factor 2) amplification] subgroup human breast cancer....
A major unmet medical need to better manage Type 2 Diabetes (T2D) is the accurate disease prediction in subjects who show glucose dysmetabolism, but are not yet diagnosed as diabetic. We investigated possibility predict/monitor progression T2D these by retrospectively quantifying blood circulating microRNAs plasma of with i) normal tolerance (NGT, n = 9); ii) impaired (IGT, 9), divided into non-progressors (NP, 5) and progressors (P, 4) based on subsequent diabetes occurrence, iii) newly (n...
Impaired glucose tolerance (IGT) is a risk factor for the development of diabetes and related complications that ensue. Early identification at-risk individuals might be beneficial to reduce or delay progression its complications. Recently, microRNAs emerged as potential biomarkers diseases. The aim present study was evaluate microRNA-21 biomarker developing in adults with IGT investigate downstream effects generation reactive oxygen species (ROS), induction manganese-superoxide dismutase-2...
The neural protein Fe65 possesses three putative protein-protein interaction domains: one WW domain and two phosphotyrosine interaction/phosphotyrosine binding domains (PID1 PID2); the most C-terminal of these (PID2) interacts in vivo with Alzheimer's β-amyloid precursor protein, whereas binds to Mena, mammalian homolog ofDrosophila-enabled protein. By trap procedure, we isolated a cDNA clone encoding possible ligand N-terminal PID/PTB (PID1). Sequence analysis this revealed that...
The two tandem phosphotyrosine interaction/phosphotyrosine binding (PID/PTB) domains of the Fe65 protein interact with intracellular region Alzheimer's beta-amyloid precursor (APP). This interaction, previously demonstrated in vitro and yeast hybrid system, also takes place vivo mammalian cells, as here by anti-Fe65 co-immunoprecipitation experiments. interaction differs from that occurring between other PID/PTB domain-containing proteins, such Shc insulin receptor substrate 1, activated...
Activated lymphocytes release nano-sized vesicles (exosomes) containing microRNAs that can be monitored in the bloodstream. We asked whether elicitation of immune responses is followed by lymphocyte-specific microRNAs. found that, upon activation vitro, human and mouse down-modulate intracellular miR-150 accumulate it exosomes. In vivo, levels increased significantly serum humans immunized with flu vaccines mice ovalbumin, this increase correlated elevation antibody titers. Immunization...