A5012137265- MicroRNA in disease regulation
- Telomeres, Telomerase, and Senescence
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer-related molecular mechanisms research
- Sphingolipid Metabolism and Signaling
- Circular RNAs in diseases
- Glycosylation and Glycoproteins Research
- Extracellular vesicles in disease
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer Research and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Occupational and environmental lung diseases
- Cell death mechanisms and regulation
- Epigenetics and DNA Methylation
- Autophagy in Disease and Therapy
- Cardiovascular Disease and Adiposity
- RNA Interference and Gene Delivery
- Mitochondrial Function and Pathology
- Cancer Cells and Metastasis
- Endoplasmic Reticulum Stress and Disease
- Immune cells in cancer
- Lipid Membrane Structure and Behavior
- Lipid metabolism and disorders
- Axon Guidance and Neuronal Signaling
- Biomarkers in Disease Mechanisms
Marche Polytechnic University
2015-2024
Istituto Nazionale di Riposo e Cura per Anziani
2009-2010
Innova (Italy)
2009
Institute of Cytology
2007
Stazione Zoologica Anton Dohrn
2004
University of Rome Tor Vergata
1994-2002
Vienna Biocenter
2000
Icahn School of Medicine at Mount Sinai
1998
Sapienza University of Rome
1998
Intracellular pathways leading from membrane receptor engagement to apoptotic cell death are still poorly characterized. We investigated the intracellular signaling generated after cross-linking of CD95 (Fas/Apo-1 antigen), a broadly expressed surface whose results in triggering cellular programs. DX2, new functional anti-CD95 monoclonal antibody was produced by immunizing mice with human CD95-transfected L cells. Crosslinking DX2 resulted activation sphingomyelinase (SMase) promyelocytic...
Gangliosides participate in development and tissue differentiation. Cross-linking of the apoptosis-inducing CD95 protein (also called Fas or APO-1) lymphoid myeloid tumor cells triggered GD3 ganglioside synthesis transient accumulation. CD95-induced accumulation depended on integral receptor “death domains” activation a family cysteine proteases caspases. Cell-permeating ceramides, which are potent inducers apoptosis, also synthesis. disrupted mitochondrial transmembrane potential (ΔΨ m ),...
The role of epigenetics in endothelial cell senescence is a cutting-edge topic ageing research. However, little known the relative contribution to pro-senescence signal propagation provided by microRNAs shuttled extracellular vesicles (EVs) released from senescent cells. Analysis microRNA and DNA methylation profiles non-senescent (control) (SEN) human umbilical vein cells (HUVECs), profiling their cognate small EVs (sEVs) large demonstrated that SEN significantly greater sEV number than...
Sodium-glucose cotransporter 2 (SGLT-2) inhibitors (i) reduce cardiovascular and renal events in patients with without type diabetes (T2D). However, the underlying mechanisms are debated. Low-grade inflammation (LGI) is a key driver of vascular complications, suggested to be attenuated by SGLT-2i animal models. Based on specific working hypothesis, here we investigated net effect LGI T2D possible mechanism. We enrolled treated either stable therapy or other glucose-lowering drugs (GLD) (n =...
Ovarian cancer is one of the most dangerous gynecologic cancers worldwide and has a high fatality rate due to diagnosis at an advanced stage disease as well recurrence occurrence chemotherapy resistance. In fact, chemoresistance weakens therapeutic effects, worsening outcome this pathology. Solute Carrier Family 7 Member 11 (SLC7A11, also known xCT) functional subunit Xc− system, anionic L-cystine/L-glutamate antiporter expressed on cell surface. SLC7A11 expression significantly upregulated...
The expression and function of CD69, a member the natural killer cell gene complex family signal transducing receptors, was investigated on human monocytes. CD69 found expressed all peripheral blood monocytes, as 28- 32-kD disulfide-linked dimer. Molecular cross-linking receptors induced extracellular Ca2+ influx, revealed by flow cytometry. resulted also in phospholipase A2 activation, detected vivo arachidonic acid release measurement from intact cells direct vitro enzymatic activity using...
Evidence on circulating microRNAs (miRNAs) is indisputably opening a new era in systemic and tissue-specific biomarker research, highlighting inter-cellular inter-organ communication mechanisms. Circulating miRNAs might be active messengers eliciting response as well non-specific "by-products" of cell activity even death; either case they have the potential to clinically relevant biomarkers for number physiopathological processes, including inflammatory responses inflammation-related...
The age-related changes of immune system functions are complex phenomena incompletely understood. acquired shows a functional decline in ability to respond new pathogens during aging, whereas serum levels inflammatory cytokines increased with age. source this systemic chronic inflammation, named inflammaging, was mainly attributed the progressive activation cells over time. However, recent studies have shown that process cellular senescence can be an important additional contributor since...
Diabetic status is characterized by chronic low-grade inflammation and an increased burden of senescent cells. Recently, the senescence-associated secretory phenotype (SASP) has been suggested as a possible source inflammatory factors in obesity-induced type 2 diabetes. However, while senescence known consequence hyperglycaemia, evidences SASP result glycaemic insult are missing. In addition, few data available regarding which cell types main SASP-spreading cells vivo. Adopting four-pronged...
Aging | doi:10.18632/aging.100693. Fabiola Olivieri, Massimiliano Bonafè, Liana Spazzafumo, Mirko Gobbi, Francesco Prattichizzo, Rina Recchioni, Fiorella Marcheselli, Lucia La Sala, Roberta Galeazzi, Maria Rita Rippo, Gianluca Fulgenzi, Sabrina Angelini, Raffaella Lazzarini, Anna Bonfigli, Francesca Brugè, Luca Tiano, Stefano Genovese, Antonio Ceriello, Massimo Boemi, Claudio Franceschi, Domenico Procopio, Roberto Testa
The development of type-2 diabetes mellitus (T2DM) and its complications is largely due to the complex interaction between genetic factors environmental influences, mainly dietary habits lifestyle, which can either accelerate or slow down disease progression. Recent findings suggest potential involvement epigenetic mechanisms as a crucial interface effects predisposition factors. common denominator promoting T2DM progression that they trigger an inflammatory response, inflammation-mediated...
Aging is a complex multidimensional, progressive remodeling process affecting multiple organ systems. While many studies have focused on studying aging across organs, assessment of the contribution individual organs to overall processes cutting-edge issue. An organ's biological age might influence other revealing multiorgan network. Recent data demonstrated similar yet asynchronous inter-organs and inter-individuals progression aging, thereby providing foundation track sources declining...
Lipid and glycolipid diffusible mediators are involved in the intracellular progression amplification of apoptotic signals. GD3 ganglioside is rapidly synthesized from accumulated ceramide after clustering death-inducing receptors triggers apoptosis. Here we show that induces dissipation ΔΨm swelling isolated mitochondria, which results mitochondrial release cytochrome c, apoptosis inducing factor, caspase 9. Soluble factors released GD3-treated mitochondria sufficient to trigger DNA...
Ceramides deriving from sphingomyelin hydrolysis are important mediators of apoptotic signals originating Fas (APO-1/CD95). However, definitive evidence for the role played by individual sphingomyelinases is still lacking. We have analyzed lymphoblastoid cell lines derived patients affected Niemann Pick disease (NPD), an autosomal recessive disorder caused loss-of-function mutations within acidic sphingomyelinase (ASM) gene. NPD lymphoblasts, which display normal neutral activity, fail to...
Diabet. Med. 28, 1388–1394 (2011) Abstract Objective The key goal of diabetes management is to prevent complications. While the patho‐physiological mechanisms responsible for complications have been extensively studied, at present it impossible predict which patient with could develop In recent years, role leukocyte telomere length in pathogenesis cardiovascular disease and Type 2 has investigated. However, studies aiming investigate telomeres development progression diabetes, as well...