A5064690451- MicroRNA in disease regulation
- Telomeres, Telomerase, and Senescence
- Extracellular vesicles in disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- COVID-19 Clinical Research Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Long-Term Effects of COVID-19
- Inflammasome and immune disorders
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Cardiovascular Disease and Adiposity
- Advanced Glycation End Products research
- COVID-19 and healthcare impacts
- Dementia and Cognitive Impairment Research
- Metabolism, Diabetes, and Cancer
- Lipoproteins and Cardiovascular Health
- Fatty Acid Research and Health
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Bone Metabolism and Diseases
- IL-33, ST2, and ILC Pathways
- Adipose Tissue and Metabolism
- Biomarkers in Disease Mechanisms
Marche Polytechnic University
2016-2025
Istituti Clinici Scientifici Maugeri
2023-2025
The role of epigenetics in endothelial cell senescence is a cutting-edge topic ageing research. However, little known the relative contribution to pro-senescence signal propagation provided by microRNAs shuttled extracellular vesicles (EVs) released from senescent cells. Analysis microRNA and DNA methylation profiles non-senescent (control) (SEN) human umbilical vein cells (HUVECs), profiling their cognate small EVs (sEVs) large demonstrated that SEN significantly greater sEV number than...
Sodium-glucose cotransporter 2 (SGLT-2) inhibitors (i) reduce cardiovascular and renal events in patients with without type diabetes (T2D). However, the underlying mechanisms are debated. Low-grade inflammation (LGI) is a key driver of vascular complications, suggested to be attenuated by SGLT-2i animal models. Based on specific working hypothesis, here we investigated net effect LGI T2D possible mechanism. We enrolled treated either stable therapy or other glucose-lowering drugs (GLD) (n =...
Diabetic status is characterized by chronic low-grade inflammation and an increased burden of senescent cells. Recently, the senescence-associated secretory phenotype (SASP) has been suggested as a possible source inflammatory factors in obesity-induced type 2 diabetes. However, while senescence known consequence hyperglycaemia, evidences SASP result glycaemic insult are missing. In addition, few data available regarding which cell types main SASP-spreading cells vivo. Adopting four-pronged...
// Fabiola Olivieri 1,2 , Liana Spazzafumo 3 Massimiliano Bonafè 4 Rina Recchioni 2 Francesco Prattichizzo 1 Fiorella Marcheselli Luigina Micolucci Emanuela Mensà Angelica Giuliani Gabriele Santini Mirko Gobbi Raffaella Lazzarini Massimo Boemi 5 Roberto Testa 6 Antonicelli 7 Antonio Domenico Procopio and Anna Rita Bonfigli 8 Department of Clinical Molecular Sciences, DISCLIMO, Università Politecnica delle Marche, Ancona, Italy Center Pathology Innovative Therapy,...
The development of type-2 diabetes mellitus (T2DM) and its complications is largely due to the complex interaction between genetic factors environmental influences, mainly dietary habits lifestyle, which can either accelerate or slow down disease progression. Recent findings suggest potential involvement epigenetic mechanisms as a crucial interface effects predisposition factors. common denominator promoting T2DM progression that they trigger an inflammatory response, inflammation-mediated...
Advanced glycation end-products (AGEs) and their interaction with the receptor for advanced (RAGE) play a pivotal role in development progression of type 2 diabetes. In this retrospective cohort study, we explored association circulating levels soluble RAGE (sRAGE) isoforms, i.e., endogenous secretory esRAGE cleaved cRAGE, AGEs respective ratios 15-year all-cause mortality diabetes.Baseline sRAGE isoforms concentration were measured by ELISA 362 patients diabetes 125 age- gender-matched...
Abstract Background The triglyceride glucose index (TyG index) is a marker of insulin resistance linked to the incidence major adverse cardiovascular events (MACE) in diverse populations. However, its long-term prognostic role type 2 diabetes (T2D) remains underexplored. This study evaluated predictive value TyG for all-cause mortality and MACE T2D over period more than 15 years. Methods A retrospective analysis was conducted on cohort 568 patients with (median age: 67 years, IQR 61–72...
// Francesco Prattichizzo 1,2 , Angelica Giuliani 1 Rina Recchioni 3 Massimiliano Bonafè 4 Fiorella Marcheselli Sabrina De Carolis Anna Campanati 5 Katia Giuliodori Maria Rita Rippo Francesca Brugè 6 Luca Tiano Carla Micucci Antonio Ceriello 2 Annamaria Offidani Domenico Procopio 1,3 and Fabiola Olivieri Department of Clinical Molecular Sciences, DISCLIMO, Università Politecnica delle Marche, Ancona, Italy Insititut d’Investigacions Biomèdiques August Pi i...
Innovative biomarkers are needed to improve the management of patients with type 2 diabetes mellitus (T2DM). Blood circulating miRNAs have been proposed as a potential tool detect T2DM complications, but lack tissue specificity, among other reasons, has hampered their translation clinical settings. Extracellular vesicle (EV)-shuttled an alternative approach. Here, we adapted immunomagnetic bead-based method isolate plasma CD31+ EVs harvest vesicles deriving from tissues relevant for...
Abstract Fibroblast Growth Factor 21 (FGF21), Differentiation 15 (GDF15), and Humanin (HN) are mitochondrial stress-related mitokines, whose role in health disease is still debated. In this study, we confirmed that their plasma levels positively correlated with age healthy subjects. However, when looking at patients type 2 diabetes (T2D) or Alzheimer’s (AD), two age-related diseases sharing a impairment, found GDF15 elevated T2D but not AD represents risk factor for complications, while...
Abstract Background Individuals with type 2 diabetes (T2D) face an increased mortality risk, not fully captured by canonical risk factors. Biological age estimation through DNA methylation (DNAm), i.e . the epigenetic clocks, is emerging as a possible tool to improve stratification for multiple outcomes. However, whether these tools predict independently of factors in subjects T2D unknown. Methods Among cohort 568 patients followed 16.8 years, we selected subgroup 50 subjects, 27 survived...
Leukocyte telomere length (LTL) shortening is found in a number of age-related diseases, including type 2 diabetes (T2DM). In this study its possible association with mortality was analyzed sample 568 T2DM patients (mean age 65.9 ± 9 years), who were followed for median 10.2 years (interquartile range 2.2). A demographic, laboratory and clinical parameters determined at baseline evaluated as risk factors. LTL measured by quantitative real-time PCR reported T/S (telomere-to-single copy gene...
Anti-virulence strategies are being explored as a novel approach to combat pathogens. Such include inhibition of surface adhesion, tissue invasion, toxin production, and/or interference with the gene regulation other virulence traits. Listeria monocytogenes, causative agent listeriosis, is facultative intracellular food pathogen characterized by wide distribution in environment. Its ability persist within biofilms and develop resistance sanitizers cause significant problems processing plants...
Residual inflammatory risk (RIR) is defined as persistent circulating levels of high sensitivity C-reactive protein (hs-CRP) >2 mg/L despite an optimal (<70 mg/dL) control LDL-cholesterol (LDL-C) and represents emerging factor for the development cardiovascular events in patients at atherosclerosis. Sparse data are available regarding prevalence RIR with type 2 diabetes (T2D) clinical variables associated hs-CRP elevation. Here, we report from a well-characterized cohort T2D (n = 511)...
In this randomized, double-blind, single-center trial (ANZCTR number ACTRN12619000436178) we aimed to investigate changes in endothelium-dependent vasodilation induced by ubiquinol, the reduced form of coenzyme Q10 (CoQ10), healthy subjects with moderate dyslipidemia. Fifty-one low-density lipoprotein (LDL) cholesterol levels 130–200 mg/dL, not taking statins or other lipid lowering treatments, (2.5%–6.0%) endothelial dysfunction as measured flow-mediated dilation (FMD) brachial artery, and...