A5025576419- Acute Myeloid Leukemia Research
- Retinoids in leukemia and cellular processes
- Histone Deacetylase Inhibitors Research
- Estrogen and related hormone effects
- Epigenetics and DNA Methylation
- Protein Degradation and Inhibitors
- MicroRNA in disease regulation
- Antioxidant Activity and Oxidative Stress
- Cancer-related molecular mechanisms research
- Acute Lymphoblastic Leukemia research
- Circular RNAs in diseases
- Muscle Physiology and Disorders
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Chronic Myeloid Leukemia Treatments
- Pluripotent Stem Cells Research
- Neuroendocrine regulation and behavior
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Genomics and Chromatin Dynamics
- Microfluidic and Bio-sensing Technologies
- Anesthesia and Neurotoxicity Research
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Chronic Lymphocytic Leukemia Research
- RNA Research and Splicing
Sapienza University of Rome
2016-2025
University Medical Center Groningen
2023
University of Groningen
2023
Istituto per la Ricerca Sociale
2012
Medico
2009
Park Foundation
2005-2009
Vita-Salute San Raffaele University
2006
Vitenparken
2005
Jewish General Hospital
1996-2004
McGill University
2002-2004
DNA methylation of tumor suppressor genes is a frequent mechanism transcriptional silencing in cancer. The molecular mechanisms underlying the specificity are unknown. We report here that leukemia-promoting PML-RAR fusion protein induces gene hypermethylation and by recruiting methyltransferases to target promoters contributes its leukemogenic potential. Retinoic acid treatment promoter demethylation, reexpression, reversion transformed phenotype. These results establish mechanistic link...
Background: Retinoids, which are derivatives of vitamin A, induce differentiation acute promyelocytic leukemia (APL) cells in vitro and patients.However, APL develop resistance to retinoic acid treatment.Arsenic trioxide (As 2 O 3 ) can clinical remission patients with APL, including those who have relapsed after treatment, by inducing apoptosis (programmed cell death) the cells.In this study, we investigated molecular mechanisms As induces acid-sensitive NB4 cells, acid-resistant these...
MicroRNAs (miRNAs) are small non-coding RNAs which contribute to the regulation of many physiological and pathological processes. Conventionally, miRNAs perform their activity in cytoplasm where they regulate gene expression by interacting a sequence-specific manner with mature messenger RNAs. Recent studies point presence nucleus. This review summarizes current findings regarding molecular activities nuclear miRNAs. These molecules can at transcriptional level directly binding DNA on...
Abstract Ex vivo amplification of human hematopoietic stem cells (HSC) without loss their self-renewing potential represents an important target for transplantation, gene and cellular therapies. Valproic acid is a safe widely used neurologic agent that acts as potent inhibitor histone deacetylase activities. Here, we show valproic addition to liquid cultures CD34+ isolated from cord blood, mobilized peripheral bone marrow strongly enhances the ex expansion different cytokine cocktails shown...
Fusion proteins involving the retinoic acid receptor alpha (RAR alpha) and PML or PLZF nuclear protein are genetic markers of acute promyelocytic leukemias (APLs). APLs with PML-RAR PLZF-RAR fusion phenotypically indistinguishable except that they differ in their sensitivity to (RA)-induced differentiation: blasts sensitive RA patients enter disease remission after treatment, while do not. We here report (i) like expression, expression blocks terminal differentiation hematopoietic precursor...
Abstract Epigenetic alterations of chromatin due to aberrant histone deacetylase (HDAC) activity and transcriptional silencing all-trans retinoic acid (ATRA) pathway are events linked the pathogenesis acute myeloid leukemia (AML) that can be targeted by specific treatments. A pilot study was carried out in eight refractory or high-risk AML patients not eligible for intensive therapy assess biological therapeutic activities HDAC inhibitor valproic (VPA) used remodel chromatin, followed...
In plants, as in mammals, mutations SNF2-like DNA helicases/ATPases were shown to affect not only chromatin structure but also global methylation patterns, suggesting a potential functional link between and epigenetic marks. The ATPase containing nucleosome remodeling deacetylase corepressor complex (NuRD) is involved gene transcriptional repression remodeling. We have previously that the leukemogenic protein PML-RARa represses target genes through recruitment of methytransferases Polycomb...