A5086205217- Microbial infections and disease research
- Effects of Environmental Stressors on Livestock
- Telomeres, Telomerase, and Senescence
- Salmonella and Campylobacter epidemiology
- Animal Disease Management and Epidemiology
- Veterinary Equine Medical Research
- Antibiotic Resistance in Bacteria
- Reproductive Physiology in Livestock
- Vector-Borne Animal Diseases
- Pharmaceutical and Antibiotic Environmental Impacts
- Animal Behavior and Welfare Studies
- Human-Animal Interaction Studies
- Animal health and immunology
- Clostridium difficile and Clostridium perfringens research
- Pharmacological Effects of Natural Compounds
- Rabies epidemiology and control
- Agriculture Sustainability and Environmental Impact
- Veterinary medicine and infectious diseases
- Sirtuins and Resveratrol in Medicine
- PARP inhibition in cancer therapy
- Zoonotic diseases and public health
- DNA Repair Mechanisms
- Mycobacterium research and diagnosis
- Genetic and phenotypic traits in livestock
- Herpesvirus Infections and Treatments
Istituti di Ricovero e Cura a Carattere Scientifico
2022-2025
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche
2014-2024
Sapienza University of Rome
2015
Istituto Sperimentale per la Frutticoltura
2015
This study investigated the mortality of dairy cows during heat waves. Mortality data (46,610 cases) referred to older than 24mo that died on a farm from all causes May 1 September 30 6-yr period (2002-2007). Weather were obtained 12 weather stations located in different areas Italy. Heat waves defined for each station as at least 3 consecutive days, (2002-2007), when daily maximum temperature exceeded 90th percentile reference distribution (1971-2000). Summer days classified wave (HW) or...
Telomere repeat binding factor 2 (TRF2) has been increasingly recognized to be involved in telomere maintenance and DNA damage response. Here, we show that TRF2 directly binds SIRT6 a independent manner this interaction is increased upon replication stress. Knockdown of up-regulates protein levels counteracts its down-regulation during response, leading cell survival. Moreover, report deactetylates vivo the TRFH domain TRF2, which turn, ubiquitylated activating ubiquitin-dependent...
Most human tumors maintain telomere lengths by telomerase, whereas a portion of them (10–15%) uses mechanism named alternative lengthening telomeres (ALT). The telomeric G-quadruplex (G4) ligand RHPS4 is known for its potent antiproliferative effect, as shown in telomerase-positive cancer models. Moreover, also able to reduce cell proliferation ALT cells, although the influence G4 stabilization on has so far been poorly investigated. Here we show that sensitivity comparable ALT-positive...
Abstract The Telomere Repeat-Binding factor 2 (TRF2) contributes to cancer progression by both telomere-dependent and independent mechanisms, including immune escape angiogenesis. Here, we found that TRF2, through its Basic domain, directly interacts with Emerin forming a complex, Lamin A/C, B1, SUN1, SUN2. Importantly, TRF2 association the inner nuclear membrane is functional proper establishment of cell polarity, finally promoting productive 1D 3D migration in triple negative breast cells...
Clostridium (C.) perfringens is the causative agent of several diseases and enteric infections in animals humans. The pathogenicity bacterium largely mediated by production a wide range toxins. Individual C. strains produce only subsets this toxin repertoire, which permits classification seven toxinotypes (A–G). In addition, variety minor toxins further characterizes single strains. aim work was to evaluate, using Polymerase Chain Reaction (PCR) assays, diversity 632 isolated Italy over 15...
The overuse of antibiotics in livestock contributes to the antibiotic resistance pandemic. assessment actual consumption is crucial limiting expansion problem effectively. aim this study was provide first qualitative and quantitative analysis antimicrobial usage using data from paper-based registers on dairy beef farms located Umbria region, Italy. Antimicrobial therapies a one-year period were collected 101 with at least 50 cattle each. Defined daily doses (DDDvet) defined course (DCDvet)...
Abstract Telomeres are nucleoprotein structures at eukaryotic chromosome termini. Their stability is preserved by a six-protein complex named shelterin. Among these, TRF1 binds telomere duplex and assists DNA replication with mechanisms only partly clarified. Here we found that poly (ADP-ribose) polymerase 1 (PARP1) interacts covalently PARylates in S-phase modifying its affinity. Therefore, genetic pharmacological inhibition of PARP1 impairs the dynamic association bromodeoxyuridine...
Subclinical mastitis (SM) is one of the most important diseases affecting dairy ewes worldwide, with negative impact on animal health, farm income and public health. Animals SM often remain untreated because disease may not be revealed. Increase in somatic cell count (SCC) positive bacteriology for pathogens milk samples are indicative but evidence only these alterations must suggest an uncertain (UM). UM defined when bacteriological examination (Latent-SM) or SCC>500 000 cells/ml...
The emergence of Clostridioides difficile as the main agent antibiotic-associated diarrhoea has raised concerns about its potential zoonotic role in different animal species. use antimicrobials is a major risk factor for C. infection. Here, we provide data on infection dairy and beef calves Umbria, region central Italy. This cross-sectional study focuses prevalence, factors, ribotypes, toxinotypes antimicrobial resistance profiles circulating ribotypes. A prevalence 19.8% (CI95%, 12–27.6%)...
TERF2/TRF2 is a pleiotropic telomeric protein that plays crucial role in tumor formation and progression through several telomere-dependent -independent mechanisms. Here, we uncovered novel function for this regulating the macroautophagic/autophagic process upon different stimuli. By using both biochemical cell biology approaches, found TERF2 binds to non-histone chromatin-associated HMGB1, interaction functional nuclear/cytoplasmic localization. Specifically, silencing of alters redox...
Summary Trichophyton verrucosum is the most common ringworm agent in cattle. Epidemiology of cattle dermatophytoses Central Italy not clear. Its diffusion among and herdsmen was investigated 20 Umbrian farms, Italy. Hairs scales were taken from 395 animals 31 workers. Typical present 71.7% under 6 months 11% over months. T. isolated 98.9% symptomatic heads prevalent dermatophyte all herds (isolated 18 farms). mentagrophytes var. found 16 eight asymptomatic young animals. Prevalence carriers...
Acute-phase proteins and trace elements are considered biomarkers of the immune response to infection, inflammation, trauma other pathological conditions, as well indicators health status productivity farm animals. Given scarcity published data on this topic, purpose study was determine serum levels amyloid A (SAA) haptoglobin, lysozyme, iron zinc in clinically healthy Lacaune sheep lactation months 3-7 ranging age from 2 6 years. The mean for SAA (12.2 µg/mL), lysozyme (1.47 (78.9 µg/dL)...
Serum protein electrophoresis (SPE) provides useful information in ruminants, but reference intervals (RI) are different from other species. There have been no reports of SPE RI for dairy sheep using agarose gel (AGE).The purpose the study was to evaluate serum concentration total (TP) and fractions determined by AGE mid-lactating ewes, establish RI, assess potential differences between Lacaune (L) Sarda (S) breeds.Blood samples were collected healthy, ewes. assessed a semi-automated system....
Carcinomas of the left colon represent a neoplasm older patients (late onset), but epidemiologic evidence has been showing an increasing incidence in 50 years or younger (early onset). In this study, we investigate pathologic and molecular features early- late-onset carcinoma colon.We selected 22 21 70 with left-sided colorectal (CRC). All samples were evaluated for features, microsatellite instability, KRAS BRAF mutations. Moreover, both groups analyzed to identify CpG island methylator...
Cystic echinococcosis (CE) is a complex zoonosis with domestic and sylvatic life-cycles, involving different intermediate definitive host species. Many previous studies have highlighted the lack of surveillance system for CE, its persistence in Italy, endemicity several Italian regions. Because absence uniform program both humans animals, disease occurrence widely underestimated. This study aimed to estimate prevalence ovine CE Italy. Survey data on Echinococcus granulosus infections sheep...