A5022421135- Telomeres, Telomerase, and Senescence
- Advanced biosensing and bioanalysis techniques
- PARP inhibition in cancer therapy
- Autophagy in Disease and Therapy
- Sirtuins and Resveratrol in Medicine
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- DNA and Nucleic Acid Chemistry
- MicroRNA in disease regulation
- Virus-based gene therapy research
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Bioinformatics and Genomic Networks
- Renal and related cancers
- Parvovirus B19 Infection Studies
- Viral Infections and Immunology Research
- Plant Virus Research Studies
- Parkinson's Disease Mechanisms and Treatments
- Advanced Glycation End Products research
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Animal Virus Infections Studies
Istituti di Ricovero e Cura a Carattere Scientifico
2019-2025
Università degli Studi della Tuscia
2022
University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca
2021
Instituto Nazionale Tumori Regina Elena
2019
Tumori Foundation
2019
Institute of Molecular Biology and Pathology
2018
Sapienza University of Rome
2018
Abstract Heterozygous germline mutations in BRCA2 predispose to breast and ovarian cancer. Contrary non-cancerous cells, where deletion causes cell cycle arrest or death, tumors carrying inactivation continue proliferate. Here we set out investigate adaptation loss of focusing on genome-wide transcriptome alterations. Human cells which expression is inhibited for 4 28 days are subjected RNA-seq analyses revealing a biphasic response abrogation. The early, acute consists downregulation genes...
KHSRP (KH-type splicing regulatory protein) is a multifunctional nucleic acid-binding protein that regulates various cellular processes, with critical roles in controlling gene expression. G-quadruplexes (G4s) are noncanonical acid structures involved essential activities, including expression, and recognized as potential therapeutic targets cancer. The biological functions of G4s mediated by proteins making their formation highly dynamic within cells. Therefore, the recognition specific...
Abstract The Telomere Repeat-Binding factor 2 (TRF2) contributes to cancer progression by both telomere-dependent and independent mechanisms, including immune escape angiogenesis. Here, we found that TRF2, through its Basic domain, directly interacts with Emerin forming a complex, Lamin A/C, B1, SUN1, SUN2. Importantly, TRF2 association the inner nuclear membrane is functional proper establishment of cell polarity, finally promoting productive 1D 3D migration in triple negative breast cells...
Abstract Background Canine parvovirus (CPV) is one of the most important pathogens dogs. Despite vaccination, CPV infections are still ubiquitous in dogs, and three antigenic variants 2a, 2b 2c variously distributed canine population worldwide. To date, no information available on circulating some European countries. The aim this study was to genetically characterise detected ten dogs with clinical signs acute gastroenteritis Romania. presence Carnivore protoparvovirus 1 DNA investigated...
TERF2/TRF2 is a pleiotropic telomeric protein that plays crucial role in tumor formation and progression through several telomere-dependent -independent mechanisms. Here, we uncovered novel function for this regulating the macroautophagic/autophagic process upon different stimuli. By using both biochemical cell biology approaches, found TERF2 binds to non-histone chromatin-associated HMGB1, interaction functional nuclear/cytoplasmic localization. Specifically, silencing of alters redox...
Kidney cancer and clear cell renal carcinoma (ccRCC) are the 16th most common cause of death worldwide. ccRCC is often metastasized at diagnosis, surgery remains main treatment; therefore, early diagnosis new therapeutic strategies highly desirable. KAT inhibitor CPTH2 lowers histone H3 acetylation induces apoptosis in colon cultured cerebellar granule neurons. In this study, we have evaluated effects on 786-O line analyzed drug targets expressed tumor tissues different grade. decreases...
Abstract Background Breast Cancer (BC) can be classified, due to its heterogeneity, into multiple subtypes that differ for prognosis and clinical management. Notably, triple negative breast cancer (TNBC) – the most aggressive BC form is refractory endocrine of target therapies. In this view, taxane-based therapy still represents elective strategy treatment tumor. However, variability in patients’ response, management TNBC an unmet medical need. Telomeric Binding Factor 2 (TRF2), a key...
Drug repositioning strategy represents a valid tool to accelerate the pharmacological development through identification of new applications for already existing compounds. In this view, we aimed at discovering molecules able trigger telomere-localized DNA damage and tumor cell death. By applying an automated high-content spinning-disk microscopy, performed screening identifying, on library 527 drugs, negatively affect expression TRF2, key protein in telomere maintenance. FK866, resulting...