A5027602447- MicroRNA in disease regulation
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Acute Myeloid Leukemia Research
- RNA Research and Splicing
- Circular RNAs in diseases
- Retinoids in leukemia and cellular processes
- Myasthenia Gravis and Thymoma
- Cancer-related gene regulation
- Histone Deacetylase Inhibitors Research
- RNA Interference and Gene Delivery
- Renal cell carcinoma treatment
- Immune cells in cancer
- Renal and related cancers
- Protein Degradation and Inhibitors
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- Endoplasmic Reticulum Stress and Disease
- Pituitary Gland Disorders and Treatments
- Ubiquitin and proteasome pathways
- Cancer Treatment and Pharmacology
- interferon and immune responses
- Glioma Diagnosis and Treatment
- Hippo pathway signaling and YAP/TAZ
- Cancer-related Molecular Pathways
Sapienza University of Rome
2016-2025
Istituto Pasteur
2018-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2019
European Institute of Oncology
2002
DNA methylation of tumor suppressor genes is a frequent mechanism transcriptional silencing in cancer. The molecular mechanisms underlying the specificity are unknown. We report here that leukemia-promoting PML-RAR fusion protein induces gene hypermethylation and by recruiting methyltransferases to target promoters contributes its leukemogenic potential. Retinoic acid treatment promoter demethylation, reexpression, reversion transformed phenotype. These results establish mechanistic link...
The Wilms tumor 1 (WT1)-associated protein (WTAP) is upregulated in many tumors, including, acute myeloid leukemia (AML), where it plays an oncogenic role by interacting with different proteins involved RNA processing and cell proliferation. In addition, WTAP also a regulator of the nuclear complex required for deposition N6-methyladenosine (m6A) into mRNAs, containing METTL3 methyltransferase. However, not clear if may have m6A-independent regulatory functions that might contribute to its...
Abstract Background In recent years, the use of ferritins as nano-vehicles for drug delivery is taking center stage. Compared to other similar nanocarriers, Archaeoglobus fulgidus ferritin particularly interesting due its unique ability assemble-disassemble under very mild conditions. Recently this was engineered get a chimeric protein targeted human CD71 receptor, typically overexpressed in cancer cells. Results used generate self-assembling hybrid nanoparticle hosting an aminic dendrimer...
Abstract Epigenetic alterations of chromatin due to aberrant histone deacetylase (HDAC) activity and transcriptional silencing all-trans retinoic acid (ATRA) pathway are events linked the pathogenesis acute myeloid leukemia (AML) that can be targeted by specific treatments. A pilot study was carried out in eight refractory or high-risk AML patients not eligible for intensive therapy assess biological therapeutic activities HDAC inhibitor valproic (VPA) used remodel chromatin, followed...
In plants, as in mammals, mutations SNF2-like DNA helicases/ATPases were shown to affect not only chromatin structure but also global methylation patterns, suggesting a potential functional link between and epigenetic marks. The ATPase containing nucleosome remodeling deacetylase corepressor complex (NuRD) is involved gene transcriptional repression remodeling. We have previously that the leukemogenic protein PML-RARa represses target genes through recruitment of methytransferases Polycomb...
Increased expression or aberrant activation of c-Myc plays an important role in leukemogenesis. Here, we show that acute myeloid leukemia (AML), directly controls the EZH2, a component Polycomb repressive complex 2, and miR-26a. miR-26a is downregulated primary blasts from AML patients and, during differentiation cells, induced together with decrease Ezh2 levels. Previously, EZH2 was shown to be regulated by at translational levels lymphomas. However, demonstrate AML, variation mainly...
// James M. Hughes 1 , Ivano Legnini Beatrice Salvatori 1, 6 Silvia Masciarelli 2 Marcella Marchioni 3 Francesco Fazi Mariangela Morlando Irene Bozzoni 3, 4, 5 Alessandro Fatica Department of Biology and Biotechnology "C. Darwin", Sapienza University Rome, Italy Anatomical, Histological, Forensic & Orthopaedic Sciences, Institute Biology, Molecular Medicine Nanobiotechnology, CNR, 4 Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Pasteur Fondazione Cenci-Bolognetti,...
Abstract In this work, we have exploited the unique properties of a chimeric archaeal-human ferritin to encapsulate, deliver and release cytochrome c induce apoptosis in myeloid leukemia cell line. The protein combines versatility 24-meric assembly cargo incorporation capability Archaeglobus fulgidus with specific binding human H CD71, “heavy duty” carrier responsible for transferrin-iron uptake. Delivery ferritin-encapsulated C Acute Promyelocytic Leukemia (APL) NB4 line, highly resistant...
MicroRNA have a central role in normal haematopoiesis and are deregulated acute myeloid leukaemia (AML). The purpose of the study was to investigate by qRT-PCR expression miRNAs involved differentiation (miR-424, miR-155, miR-223, miR-17-5p) 48 patients with cytogenetically AML well characterized for NPM1 and/or FLT3 mutations. Three types normalization were used data validation. We found that miR-424 down-modulated AMLs NPM1mutA regardless status. On contrary, miR-155 showed up-regulation...
Blocks in genetic programs required for terminal myeloid differentiation and aberrant proliferation characterize acute leukemia (AML) cells. 1,25-Dihydroxy-vitamin D3 (VitD3) arrests of AML cells induces their into mature monocytes. In a previous study, we showed that miR-26a was induced upon VitD3-mediated monocytic differentiation. Here, identify E2F7 as novel target miR-26a. We show significantly promotes cell cycle progression inhibits also demonstrate binds the cyclin-dependent kinase...