A5035574540- Iron Metabolism and Disorders
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Photochromic and Fluorescence Chemistry
- Bacterial Genetics and Biotechnology
- Metabolism and Genetic Disorders
- Amino Acid Enzymes and Metabolism
- Bacteriophages and microbial interactions
- Trace Elements in Health
- Receptor Mechanisms and Signaling
- RNA Interference and Gene Delivery
- Enzyme Structure and Function
- Hemoglobinopathies and Related Disorders
- Electron Spin Resonance Studies
- Lanthanide and Transition Metal Complexes
- Genomics and Phylogenetic Studies
- Peptidase Inhibition and Analysis
- Biotin and Related Studies
- HER2/EGFR in Cancer Research
- Magnetism in coordination complexes
- Retinal Development and Disorders
- Neonatal Health and Biochemistry
- Antibiotic Resistance in Bacteria
- Photoreceptor and optogenetics research
- RNA Research and Splicing
Sapienza University of Rome
2016-2020
Italian Institute of Technology
2019
University of Lisbon
2014-2015
Abstract In this work, we have exploited the unique properties of a chimeric archaeal-human ferritin to encapsulate, deliver and release cytochrome c induce apoptosis in myeloid leukemia cell line. The protein combines versatility 24-meric assembly cargo incorporation capability Archaeglobus fulgidus with specific binding human H CD71, “heavy duty” carrier responsible for transferrin-iron uptake. Delivery ferritin-encapsulated C Acute Promyelocytic Leukemia (APL) NB4 line, highly resistant...
Lipopolysaccharides are major constituents of the extracellular leaflet in bacterial outer membrane and form an effective physical barrier for environmental threats antibiotics Gram-negative bacteria. The last step LPS insertion via Lpt pathway is mediated by LptD/E protein complex. Detailed insights into architecture LptDE transporter complexes have been derived from X-ray crystallography. However, no structure a laterally open LptD transporter, transient state that occurs during release,...
Galactose-1-phosphate uridylyltransferase (GALT) is a key enzyme in galactose metabolism, particularly important the neonatal period due to ingestion of galactose-containing milk. GALT deficiency results genetic disorder classic galactosemia, whose pathophysiology still not fully elucidated. Whereas galactosemia has been hypothesized result from misfolding, thorough functional-structural characterization most prevalent variants was lacking, hampering development alternative therapeutic...
Human ferritins have been extensively studied to be used as nanocarriers for diverse applications and could represent a convenient alternative targeted delivery of anticancer drugs imaging agents. However, the most relevant limitation their is need highly acidic experimental conditions during initial steps particle/cargo assembly, process that affect both drug stability complete reassembly ferritin cage. To overcome this issue unique assembly Archaeoglobus fulgidus was genetically engineered...
Ferritin H-homopolymers have been extensively used as nanocarriers for diverse applications in the targeted delivery of drugs and imaging agents, due to their unique ability bind transferrin receptor (CD71), highly overexpressed most tumor cells. In order incorporate novel fluorescence properties, we fused a lanthanide binding tag (LBT) C-terminal end mouse H-chain ferritin, HFt. The HFt-LBT possesses one high affinity Terbium site per each 24 subunits provided by six coordinating aminoacid...
Classic galactosemia is a rare genetic disease of the galactose metabolism, resulting from deficient activity galactose-1-phosphate uridylyltransferase (GALT). The current standard care lifelong dietary restriction galactose, which however fails to prevent development long-term complications. Structural-functional studies demonstrated that most prevalent GALT mutations give rise proteins with increased propensity aggregate in solution. Arginine known stabilizer aggregation-prone proteins,...
Pyrene fluorescence changes upon ferritin self-assembly allowed to establish the kinetic and thermodynamic details of archaeal ferritins oligomerization mechanism was successfully visualized<italic>in vitro</italic>by two photon microscopy.
Abstract G protein-coupled receptors (GPCRs) are the largest family of cell surface in humans. The binding and dissociation ligands tunes inherent conformational flexibility these important drug targets towards distinct functional states. To trigger such protein-ligand interaction dynamics within human adenosine A 2A receptor, we designed seven photochemical affinity switches derived from anti-Parkinson’s istradefylline. In a rational approach based on UV/Vis spectroscopy, time-resolved...
Photoisomerization of ligands is a key process in the field photopharmacology. Thus, kinetics and efficiency this initial photoreaction are great importance but can be influenced by molecular environment binding pocket resulting confinement reaction pathway. In study, we investigated photoisomerization an azobenzene derivative anti-Parkinson's drug istradefylline. To identify impact pocket, ligand was examined solution bound to its target protein, A
G protein-coupled receptors (GPCRs) are the largest family of cell surface in humans. The binding and dissociation ligands tunes inherent conformational flexibility these important drug targets towards distinct functional states. Here we show how to trigger resolve protein-ligand interaction dynamics within human adenosine A
Abstract Lipopolysaccharides (LPS) are major constituents of the extracellular leaflet in bacterial outer membrane and form an effective physical barrier for environmental threats antibiotics Gram-negative bacteria 1 . The last step LPS insertion via Lpt pathway is mediated by LptD/E protein complex 2 Despite detailed insights from X-ray crystallography into architecture LptDE transporter complexes 3–5 , no structure a laterally open LptD has been described, transient state that occurs...