A5009349322- Photochromic and Fluorescence Chemistry
- Receptor Mechanisms and Signaling
- Enzyme Catalysis and Immobilization
- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Protein Kinase Regulation and GTPase Signaling
- Catalysis for Biomass Conversion
- Biochemical and Structural Characterization
- Neuroscience and Neuropharmacology Research
- Enzyme Structure and Function
- Mass Spectrometry Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Metabolism, Diabetes, and Cancer
University of Graz
2022-2023
Ca' Foscari University of Venice
2019
Native and promiscuous catalytic activities of flavin-dependent Old Yellow Enzymes (OYEs) reported to date are initiated by the reduced flavin upon electron transfer. As a rare exception, isomerization nonactivated C═C bond was shown be hydride-independent with two nonstereoselective yeast OYEs. Here, we report asymmetric prochiral model substrate, γ-methyl β,γ-butenolide, corresponding (R)- (S)-enantiomers α,β-butenolide in up >99% ee stereocomplementary OYEs algal fungal origin,...
ABSTRACT Heterotrimeric G proteins play a central role in cellular signaling, acting as switchable molecular regulators. Consequently, pharmacological agents to control protein activity are of utmost importance advance our understanding this signal transduction system. The natural depsipeptides FR900359 (FR) and YM-254890 (YM) two highly specific widely used inhibitors heterotrimeric Gq/11 proteins. These compounds have traditionally been understood inhibit GDP dissociation by preventing the...
Abstract G protein-coupled receptors (GPCRs) are the largest family of cell surface in humans. The binding and dissociation ligands tunes inherent conformational flexibility these important drug targets towards distinct functional states. To trigger such protein-ligand interaction dynamics within human adenosine A 2A receptor, we designed seven photochemical affinity switches derived from anti-Parkinson’s istradefylline. In a rational approach based on UV/Vis spectroscopy, time-resolved...
G protein-coupled receptors (GPCRs) are the largest family of cell surface in humans. The binding and dissociation ligands tunes inherent conformational flexibility these important drug targets towards distinct functional states. Here we show how to trigger resolve protein-ligand interaction dynamics within human adenosine A
Abstract The formal asymmetric and stereodivergent enzymatic reduction of α‐angelica lactone to both enantiomers γ‐valerolactone was achieved in a one‐pot cascade by uniting the promiscuous stereoselective isomerization activity Old Yellow Enzymes with their native reductase activity. In addition running one enzyme for each catalytic step, bifunctional isomerase‐reductase biocatalyst designed fusing two Enzymes, thereby generating an unprecedented case artificial catalyzing nonactivated C=C...