A5084575138- Hepatitis Viruses Studies and Epidemiology
- Hepatitis B Virus Studies
- Metabolism and Genetic Disorders
- Viral gastroenteritis research and epidemiology
- Folate and B Vitamins Research
- Hepatitis C virus research
- Viral Infections and Immunology Research
- Mitochondrial Function and Pathology
- Lysosomal Storage Disorders Research
- Neonatal Health and Biochemistry
- Liver Disease and Transplantation
- Amino Acid Enzymes and Metabolism
- Central Venous Catheters and Hemodialysis
- Cancer, Hypoxia, and Metabolism
- Cellular transport and secretion
- Glycosylation and Glycoproteins Research
- Liver Disease Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- HIV/AIDS Research and Interventions
- Peroxisome Proliferator-Activated Receptors
- Autoimmune and Inflammatory Disorders Research
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Cardiac Arrhythmias and Treatments
- Lipid metabolism and disorders
- Ion Transport and Channel Regulation
Los Andes Peruvian University
2024
Centro Hospitalar Lisboa Norte
2018-2024
Santa Casa da Misericórdia de Almeirim
2024
Hospital de Santa Maria
2013-2023
University of Lisbon
2003-2023
Hospital Prof. Dr. Fernando Fonseca
2017-2023
Instituto Politécnico de Santarém
2022
Lister Hospital
2021
Universidade Nova de Lisboa
2021
Fundação Oswaldo Cruz
2007-2019
The rat cDNA sequence of PC4 (rPC4), representing a new member the Kex2/subtilisin-like proprotein convertases, demonstrated presence at least three rPC4 mRNAs resulting in production rPC4-A (654 amino acids), rPC4-B (619 and rPC4-C (609 acids) with different C-terminal sequences. Analogous to PC4, cDNAs were also found for mouse PC4. observed molecular diversity mRNA possibly results from differential splicing and/or exon skipping parent gene. mRNA, major form 2.8 kilobases, was highly...
The complete genome of a hepatitis B virus (HBV) from Brazil that expressed the subtype adw4 HBV surface antigen (HBsAg) was cloned and sequenced. genome, termed w4B, consists 3215 bp. overall genetic organization typical hepadnaviruses with four open reading frames including preC region found to be conserved. When comparing w4B sequence 19 genomes it was, however, more divergent (15%) than any other thus far reported. Until now, no 11% divergence has been Distinct five known genotypes A E,...
Hepatitis B virus (HBV) serum markers (HBsAg, anti-HBs, anti-HBc) and antihepatitis C antibody (anti-HCV) were prospectively followed in haemodialysis CAPD patients. From January 1987 to 1990, 185 patients on 124 analysed. Among susceptible HBV (69 70 CAPD), there 17 HBsAg seroconversions (0.19/patient-year) 1 (0.01/patient-year). A Cox proportional hazards model showed that treatment was the only risk factor significantly associated with infection, thus suggesting transmission through...
Sporadic acute hepatitis E cases occurring in non-endemic areas have been associated to genotypes 3 and 4 of virus. Several studies demonstrated the relationship among human animals strains, mostly pigs deers, from respective characterizing zoonosis. Circulation genotype HEV Brazilian swine herds already demonstrated. Nevertheless, no confirmed reported date Brazil.A study was developed attempt identification retrospective carried out with 64 serum samples patients non-A-C performed identify...
Introduction: Rare disorders that are genetically and clinically heterogeneous, such as mitochondrial diseases (MDs), have a challenging diagnosis. Nuclear genes codify most proteins involved in biogenesis, despite all mitochondria having their own DNA. The development of next-generation sequencing (NGS) technologies has revolutionized the understanding many pathogenesis MDs. In this new genetic era, using NGS approach, we aimed to identify etiology for suspected MD cohort 450 Portuguese...
A one-year survey was conducted to examine hepatitis virus (HAV) prevalence, distribution of genotypes and their relationship bacterial indicators in raw treated sewage samples.Fifty samples (raw = 25 25) were collected twice monthly from one treatment plant Rio de Janeiro. Virus concentration performed by adsorption an electronegative membrane followed ultrafiltration. Viral RNA detected nested reverse transcriptase-polymerase chain reaction (RT-PCR) real-time PCR positive products directly...
The aim of this study was to determine the occurrences group A rotavirus (RVA), norovirus (NoV) and human adenovirus (HAdV) in surface waters an urban lagoon (Rodrigo de Freitas Lagoon) city Rio Janeiro, Brazil. During one year surveillance, water samples were obtained from other interconnected ecosystems (river beach). concentrated using adsorption-elution method with a negatively charged membrane tested by qualitative quantitative polymerase chain reaction assays. RVA most prevalent virus...
Epidemiological studies found that hepatitis E virus genotype 3 (HEV-3) infection was associated with chronic and cirrhosis in immunocompromised patients. Our study aimed to investigate the relationship between host immunosuppressive status occurrence of HEV-related hepatitis. Here we describe a successful experimental study, using cynomolgus monkeys previously treated tacrolimus, potent calcineurin inhibitor immunosuppressant, infected Brazilian HEV-3 strain isolated from naturally pigs....
Mediterranean regions are among the most important areas for global grape production, characterized by dry climates and frequent challenges associated with soil salinity. In these environments, chloride toxicity is a major factor limiting vine growth fruit quality. Despite critical role of exclusion in salinity tolerance, genetic mechanisms underlying this trait remain poorly understood. study, we leveraged one largest Vitis germplasm collections, comprising 335 accessions from 18 wild...
Grape cluster compactness is a key trait that influence fruit quality, yield, and disease susceptibility. Understanding the genetic basis of this essential for optimizing vineyard management improving grapevine cultivars. In study, we performed quantitative locus (QTL) mapping to identify genomic regions associated with architecture yield components in bi-parental population derived from Vitis vinifera cv. Riesling crossed Cabernet Sauvignon. A total 138 full-sibling progeny were evaluated...
Abstract Hepatitis A virus (HAV) isolates from around the world have been classified into seven genotypes (I–VII). Most human strains belong to genotype I, which has divided two subgenotypes, and B. South America provided a small number of studied at genome level. In present study, IgM anti‐HAV antibodies were detected in 116 out 250 (46%) serum samples collected consecutive patients with acute hepatitis referred Brazilian Reference Center for Viral Hepatitis, Rio de Janeiro. RNA extracted...
Abstract Hepatitis B virus (HBV) genomes have been classified into six groups, A–F, group F being the most divergent. South America has provided smallest number of strains studied at genome level. The only HBV strain from this region sequenced completely in F, and contains divergent presently known [Naumann et al. (1983): Journal General Virology 74:1627–1632]. To evaluate genetic relatedness between isolated rest world, a restriction endonuclease analysis was carried out on 14 (4 adw 2, 3...
Abstract Pathogenic variants in the NGLY1 gene are associated with a Congenital Disorder of Deglycosylation (CDDG) characterized by delays reaching developmental milestones, complex hyperkinetic movement disorder, transient elevation transaminases, and alacrima or hypolacrima. To date, only few cases deficiency have been identified reported literature. This report highlights first child non‐consanguineous parents no relevant family history who presented hypotonia poor weight gain since...
There are no data concerning the genotypic analysis of hepatitis B virus (HBV) infection in Brazilian dialysis centers. Serum samples from all hemodialysis patients (n = 282) Goiânia City, Central Brazil, were tested for surface antigen (HBsAg). An overall prevalence 12.0% was found, ranging 0 to 33.3% depending on Positive 34) submitted serological subtyping by monoclonal ELISA and HBV DNA detection polymerase chain reaction (PCR). Among 30 PCR-positive samples, 26 genotyped use line probe...
Abstract A serological and molecular study of hepatitis B virus (HBV) infection was carried out in dialysis units Central Brazil. Between 1995 1999, serum samples from all HBsAg‐positive hemodialysis patients ( n = 43) were tested for HBeAg/anti‐HBe subtyping by monoclonal ELISA. HBV DNA detected PCR positive genotyped restriction fragment polymorphism pattern (RFLP) methodology. TheHBsAg prevalence declined this population during the survey period (12–5.8%). HBeAg anti‐HBe 23 (53.5%) 18...
Using a probe consisting of the full-length cDNA sequence mouse pro-hormone convertase PC1 (mPC1), we isolated from lambda gt10 human pituitary library number contiguous clones, which composite 3.3-kb defined complete coding (hPC1). The hPC1 encodes protein containing 753 amino acids and potentially two N-glycosylation sites, one carboxy-terminal amidation site, cAMP-dependent kinase Ser phosphorylation tyrosine an ArgGlyAsp (RGD) sequence. Like mPC1, exhibits amphipathic domain involved in...
Summary This work studied the replication sites of hepatitis A virus (HAV) in cynomolgus monkeys ( Macaca fascicularis ) after intravenous inoculation. The were inoculated with Brazilian strain (HAF‐203). Monkeys euthanized on days 15, 30, 45 and 60 postinoculation (pi). Liver samples, submandibular salivary gland, mesenteric lymph node tonsils removed for virological pathological evaluation. Immunofluorescence analyses liver gland sections using confocal laser scanning microscopy revealed...
Abstract A limited number of hepatitis virus (HAV) isolates from South America have been characterised at the genomic level. IgM anti‐HAV positive serum samples collected patients with living in five geographical regions Brazil (North, Northeast, Central, South, and Southeast) were used to obtain HAV determine their genetic relatedness. Of 232 case isolates, sequence data obtained VP1/2A junction region genome. All classified genotype I; 231 belonged subgenotype IA, one IB. four States...