A5007557827- Nanoparticle-Based Drug Delivery
- Radiopharmaceutical Chemistry and Applications
- Prostate Cancer Treatment and Research
- Toxin Mechanisms and Immunotoxins
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Transgenic Plants and Applications
- Cell Adhesion Molecules Research
- Iron Metabolism and Disorders
- Mass Spectrometry Techniques and Applications
- Laser-Ablation Synthesis of Nanoparticles
- Glycosylation and Glycoproteins Research
- Biosensors and Analytical Detection
- Photodynamic Therapy Research Studies
- Peptidase Inhibition and Analysis
- Prostate Cancer Diagnosis and Treatment
- Protein purification and stability
- Lipid Membrane Structure and Behavior
- Cancer Research and Treatments
- Medical Imaging Techniques and Applications
University of Padua
2023-2025
University of Verona
2014-2023
City of Hope
2011
University Hospital Cologne
2011
University of Bologna
2006
École Polytechnique Fédérale de Lausanne
2005
Institute of Molecular Science and Technologies
2005
Osaka University
2005
Azienda Ospedaliera Universitaria Integrata Verona
2004-2005
Myeloid cells can restrain antitumor immunity by metabolic pathways, such as the degradation of l-arginine, whose concentrations are regulated arginase 1 (ARG1) enzyme. Results from preclinical studies indicate important role arginine metabolism in pancreatic ductal adenocarcinoma (PDAC) progression, suggesting a potential for clinical application; however, divergent evolution ARG1 expression and function rodents humans has restricted translation. To overcome this dichotomy, here, we show...
Diagnostic approaches based on multimodal imaging are needed for accurate selection of the therapeutic regimens in several diseases, although dose administered contrast drugs must be reduced to minimize side effects. Therefore, large efforts deployed development agents (MCAs) that permit complementary visualization same diseased area with different sensitivity and spatial resolution by applying multiple diagnostic techniques. Ideally, MCAs should also allow tissues high during surgical...
A novel human ferritin-based nanocarrier, composed of 24 modified monomers able to auto-assemble into a protein cage, was produced and used as selective carrier anti-tumor payloads. Each monomer derives from the genetic fusion two distinct modules, namely heavy chain ferritin (HFt) stabilizing/protective PAS polypeptide sequence rich in proline (P), serine (S), alanine (A) residues. Two genetically fused constructs containing polymers with 40- 75-residue lengths, respectively, were compared....
The interleukin-6 (IL-6) and the chemokine CCL5 are implicated in development progression of several forms tumours including that prostate. expression prostate specific membrane antigen (PSMA) is augmented high-grade metastatic tumors. Observations clinical behaviour tumors suggest increased secretion IL-6 higher PSMA may be correlated. We hypothesized could endowed with signalling properties its stimulation might impact on regulation gene CCL5. herein demonstrate cross-linking cell surface...
Multifunctional iron oxide (FeOx) magnetic nanoparticles (MNPs) are promising items for biomedical applications. They studied as theranostic agents cancer treatment, selective probes bioanalytical assays, controllable carriers drug delivery and biocompatible tools cell sorting or tissue repair. Here we report a new method the synthesis in water of FeOx–MNPsvia top-down physical technique consisting Laser Ablation Synthesis Solution (LASiS). LASiS is green that does not require chemicals...
Both radionuclide imaging and near-infrared fluorescent (NIRF) have a high sensitivity to detect tumors in vivo. The combination of these modalities using dual-labeled antibodies may allow both preoperative intraoperative tumor localization be used image-guided surgery ensure complete resection tissue. Here, we evaluated the potential dual-modality prostate cancer with monoclonal antibody D2B, directed against an extracellular domain prostate-specific membrane antigen (PSMA). For studies,...
A novel electrochemiluminescence immunosensor based on carbon nanotubes for PSMA detection in very complex matrixes exceeds the state of-the-art.
Immunology-based interventions have been proposed as a promising curative chance to effectively attack postoperative minimal residual disease and distant metastatic localizations of prostate tumors. We developed chimeric antigen receptor (CAR) construct targeting the human prostate-specific membrane (hPSMA), based on novel high affinity specific mAb. As transfer method, we employed last-generation lentiviral vectors (LV) carrying synthetic bidirectional promoter capable robust coordinated...
Prostate cancer (PCa) has become the most common among males in Europe and USA. Adoptive immunotherapy appears a promising strategy to control advanced stages of disease by specifically targeting tumor, particular through chimeric antigen receptor T (CAR-T) cell therapy. Despite advancements CAR-T technology treatment hematological malignancies, solid tumors still represent challenge. To overcome current limits, other cellular effectors than lymphocytes are under study as possible candidates...
Despite the multitude of therapeutic agents available to treat prostate cancer (PC), there are still no effective and safe measures tumor. It remains a challenge develop simple approach target PC with specific antibodies. In our study, D2B monoclonal antibodies against prostate-specific membrane antigen (PSMA) were used. We investigated functionalization gold nanoparticles (AuNPs) generate favorable physicochemical biological properties that mediate binding PC. For this purpose, AuNPs size...
Gold nanocorals (AuNC) are obtained by a “green” method consisting of laser irradiation, followed anisotropic self-assembly without templating agents. The AuNC dispersion has broadband plasmon absorption from the visible to near infrared region, unitary light-to-heat conversion efficiency, versatile surface chemistry and biocompatibility.
Monitoring Prostate Cancer (PCa) biomarkers is an efficient way to diagnosis this disease early, since it improves the therapeutic success rate and suppresses PCa patient mortality: for reason a powerful analytical technique such as electrochemiluminescence (ECL) already used application, but its widespread usability still hampered by high cost of commercial ECL equipment. We describe innovative approach selective sensitive detection biomarker sarcosine, obtained synergistic...
Immunosuppression is a hallmark of tumor progression, and treatments that inhibit or deplete monocytic myeloid-derived suppressive cells could promote anti-tumor immunity. c-FLIP central regulator caspase-8-mediated apoptosis necroptosis. Here we show low-dose cytotoxic chemotherapy agents cause linked to down-regulation selectively in monocytes. Enforced expression viral FLIP rescues monocytes from cytotoxicity concurrently induces potent immunosuppressive activity, T cell cultures vivo...
Rationale: Prostate cancer (PCa) recurrences after surgery frequently occur. To improve the outcome surgical resection of tumor, theranostic multimodal anti-PSMA targeting agent 111In-DTPA-D2B-IRDye700DX was developed and characterized for both pre- intra-operative tumor localization eradication (residual) tissue by PSMA-targeted photodynamic therapy (tPDT), which is a highly selective treatment based on molecules conjugated to photosensitizers that can induce cell destruction upon exposure...
There is presently no effective therapy for relapsing, metastatic, androgen-independent prostate cancer. Immunotherapy with monoclonal antibody-vehicled toxins (Immunotoxins, ITs) may be a promising novel treatment option the management of cancer in these cases.Three anti-prostate specific membrane antigen (anti-PSMA) monoclonals (J591, PEQ226.5, and PM2P079.1) were cross-linked to ricin A-chain (RTA; native or recombinant), their cytotoxic effects investigated monolayer three-dimensional...
Doxorubicin is employed alone or in combination for the treatment of several hematological and solid malignancies; despite its efficacy, there are associated cardiotoxicity limits both application patients with heart disease risk factors also long-term use. HFt-MP-PAS40 a genetically engineered human ferritin heavy chain (HFt)-based construct able to efficiently entrap deliver doxorubicin cancer cells. HF-MP-PAS contains short motif sequence (defined as MP) responsive proteolytic cleavage by...
Abstract We aimed to evaluate a fluorescent-labeled single chain variable fragment (scFv) of the anti-PSMA antibody as specific probe for detection prostate cancer by in vivo fluorescence imaging. An orthotopic model was generated injecting LNCaP cells into lobe. ScFvD2B, high affinity fragment, labeled using near-infrared fluorophore generate imaging (X770-scFvD2B). PSMA-unrelated scFv-X770 used control. Probes were injected intravenously mice with tumors and monitored molecular tomography...
The stromal vascular fraction (SVF) consists of a heterogeneous population stem and cells, generally obtained from adipose tissue by enzymatic digestion. For human cell-based therapies, mechanical process methods to obtain SVF represent an advantageous approach because they have fewer regulatory restrictions for their clinical use. aim this study was characterize novel commercial system obtaining without substantial manipulations. Lipoaspirate samples collected 27 informed patients were...
Abstract The mitochondrial chaperone TRAP1 is a key regulator of cellular homeostasis and its activity has important implications in neurodegeneration, ischemia cancer. Recent evidence indicated that mutations are involved several disorders, even though the structural basis for impact point on functions never been studied. By exploiting modular structure-based framework molecular dynamics simulations, we investigated effect five structure stability. Each mutation differentially impacts...
D2B is a new monoclonal antibody directed against an extracellular domain of prostate‐specific membrane antigen (PSMA), which overexpressed in prostate cancer. The potential IgG, and F(ab′) 2 Fab fragments this for targeting cancer was determined mice bearing subcutaneous xenografts. optimal time point imaging biodistribution microSPECT studies with 111 In‐D2B In‐capromab pendetide, PSMA‐expressing LNCaP PSMA‐negative PC3 tumors at several points after injection. All In‐labeled formats...