A5024937751- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Chemokine receptors and signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Cellular Mechanics and Interactions
- Immune Response and Inflammation
- Celiac Disease Research and Management
- Monoclonal and Polyclonal Antibodies Research
- Microscopic Colitis
- Immune cells in cancer
- Protein Kinase Regulation and GTPase Signaling
- Protease and Inhibitor Mechanisms
- Helicobacter pylori-related gastroenterology studies
- CAR-T cell therapy research
- Cytokine Signaling Pathways and Interactions
- Cancer Immunotherapy and Biomarkers
- Dermatology and Skin Diseases
- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- HER2/EGFR in Cancer Research
- Signaling Pathways in Disease
- Urticaria and Related Conditions
- interferon and immune responses
- Immunotherapy and Immune Responses
Sapienza University of Rome
2012-2025
Policlinico Umberto I
2013-2021
Italian Institute of Technology
2015
Zero to Three
2012
Istituto Pasteur
1998-2009
New York University
1994-2000
Memorial Sloan Kettering Cancer Center
1999
Fondazione IRCCS Istituto Nazionale dei Tumori
1998
Istituto Neurologico Mediterraneo
1998
Kaplan (United States)
1996-1997
We provide evidence that a class of integrins combines with the adaptor Shc and thereby Grb2. Coimmunoprecipitation mutagenesis experiments indicate recruitment is specified by extracellular or transmembrane domain integrin α subunit suggest this process mediated caveolin. Mutagenesis dominant-negative inhibition studies reveal necessary sufficient for activation MAP kinase pathway in response to ligation. Mitogens Shc-activating cooperate promote transcription from Fos serum element transit...
The chemokine CXC ligand 8 (CXCL8)/IL-8 and related agonists recruit activate polymorphonuclear cells by binding the receptor 1 (CXCR1) CXCR2. Here we characterize unique mode of action a small-molecule inhibitor (Repertaxin) CXCR1 Structural biochemical data are consistent with noncompetitive allosteric interaction between Repertaxin, which, locking in an inactive conformation, prevents signaling. Repertaxin is effective cell recruitment vivo protects organs against reperfusion injury....
To evaluate the role of local inflammation in pathogenesis and postoperative recurrence chronic subdural hematoma (CSDH), authors conducted an investigation a selected group patients who could clearly recall traumatic event did not have other risk factors for CSDH. Inflammation was analyzed by measuring concentration proinflammatory inflammatory cytokines interleukin (IL)-6 IL-8. The also investigated possible relationship between high levels that were measured CSDH.A prospective study...
Upon ligand binding, the alpha6beta4 integrin becomes phosphorylated on tyrosine residues and combines sequentially with adaptor molecules Shc Grb2, linking to ras pathway, cytoskeletal elements of hemidesmosomes. Since is expressed in a variety tissues regulated by EGF receptor (EGFR), we have examined effect signaling functions alpha6beta4. Experiments immunoblotting anti-phosphotyrosine antibodies immunoprecipitation followed phosphoamino acid analysis phosphopeptide mapping showed that...
Abstract Mice exposed to standard (SE) or enriched environment (EE) were transplanted with murine human glioma cells and differences in tumour development evaluated. We report that EE exposure affects: (i) size, increasing mice survival; (ii) establishment, proliferation invasion; (iii) microglia/macrophage (M/Mφ) activation; (iv) natural killer (NK) cell infiltration (v) cerebral levels of IL-15 BDNF. Direct infusion BDNF the brain significantly reduces growth. demonstrate increases...
The interleukin-6 (IL-6) and the chemokine CCL5 are implicated in development progression of several forms tumours including that prostate. expression prostate specific membrane antigen (PSMA) is augmented high-grade metastatic tumors. Observations clinical behaviour tumors suggest increased secretion IL-6 higher PSMA may be correlated. We hypothesized could endowed with signalling properties its stimulation might impact on regulation gene CCL5. herein demonstrate cross-linking cell surface...
Microglial cells participate in brain development and influence neuronal loss synaptic maturation. Fractalkine is an important chemokine whose expression increases during that can microglia function via the fractalkine receptor, CX3CR1. Mice lacking Cx3cr1 show a variety of defects thought to be result deficient function. Activation CX3CR1 for proper migration sites injury into development. However, little known about how modulates microglial properties Here we examined morphology, response...
In 2012, European Society of Pediatric Gastroenterology, Hepatology, and Nutrition published novel guidelines on celiac disease (CD) diagnosis. Symptomatic children with serum anti-transglutaminase (anti-tTG) antibody levels ≥10 times upper limit normal (ULN) could avoid duodenal biopsies after positive HLA test anti-endomysial antibodies (EMAs). So far, both asymptomatic symptomatic patients anti-tTG titer <10 ULN should undergo endoscopy to confirm The aim this study was assess the...
In glioma, microglia and infiltrating macrophages are exposed to factors that force them produce cytokines chemokines, which contribute tumor growth maintaining a pro-tumorigenic, immunosuppressed microenvironment. We demonstrate housing glioma-bearing mice in enriched environment (EE) reverts the immunosuppressive phenotype of myeloid cells, by modulating inflammatory gene expression. Under these conditions, branching patrolling activity cells is increased, their phagocytic promoted....
Wnt1-inducible signaling pathway protein 1 (WISP1) is a novel adipokine participating in adipose tissue (AT) dysfunction; so far, no data on WISP1 diabetes are available. To evaluate plasma subjects with type 2 (T2D) and its correlates linked to AT inflammation. For this cross-sectional study, 97 consecutive dysmetabolic patients were recruited at the outpatient clinics of Sapienza University Rome; 71 them had T2D, (n = 35) or without 36) obesity, 26 obese diabetes. Twenty-one normal-weight...
Background Local allergic rhinitis (LAR) is a phenotype of that has been poorly studied in children. It characterized by the same symptoms but with absence markers systemic atopy. Objective To identify children affected LAR and to analyze pathogenesis this disease. We chose focus our attention on interleukin (IL) thymic stromal lymphopoietin (TSLP). Methods enrolled 20 nonallergic (negative skin-prick test results serum specific immunoglobulin E [sIgE] values). Each patient underwent nasal...
Tat, the transactivator of HIV-1 gene expression, is released by acutely HIV-1-infected T-cells and promotes adhesion, migration, growth inflammatory cytokine-activated endothelial Kaposi's sarcoma cells. It has been previously demonstrated that these effects Tat are due to its ability bind through arginine-glycine-aspartic (RGD) region alpha5beta1 alphavbeta3 integrins. However, signaling pathways linking regulation cellular functions incompletely understood. Here, we report ligation on...
Upon noxious insults, cells of the brain parenchyma activate endogenous self-protective mechanisms to counteract damage. Interplay between microglia and astrocytes can be determinant build a physiological response stimuli arisen from injury or stress, thus understanding cross talk would helpful elucidate role glial in protective might contribute development new strategy mobilize such program reduce cell death. Here we demonstrate that chemokines CX3CL1 CXCL16 are molecular players...
// Giuseppina D'Alessandro 1, 2 , Alfonso Grimaldi 1 Chece Alessandra Porzia 3 Vincenzo Esposito 2, 4 Antonio Santoro Maurizio Salvati 5 Fabrizio Mainiero 6 Davide Ragozzino Silvia Di Angelantonio 7 Heike Wulff 8 Myriam Catalano Cristina Limatola 9 Department of Physiology and Pharmacology, Sapienza University Rome, Italy IRCCS Neuromed, Pozzilli, Molecular Medicine, Neurology Psychiatry, Science Medical Surgical Biotechnology, Experimental Center for Life Nanoscience, Istituto Italiano di...
Recent evidence indicates that integrin engagement results in the activation of biochemical signaling events important for regulating different cell functions, such as migration, adhesion, proliferation, differentiation, apoptosis, and specific gene expression. Here, we report β1 ligation on human natural killer (NK) cells Ras/mitogen-activated protein kinase pathways. Formation Shc–growth factor receptor–bound 2 (Grb2) Shc–proline-rich tyrosine 2–Grb2 complexes are receptor-proximal...
Recent evidence indicates that integrin ligation results in activation of focal adhesion kinase (pp125FAK), the prototype a new subfamily nonreceptor protein tyrosine (PTK), including FAKB and proline-rich 2 (PYK-2), also termed cell kinase-beta or related kinase. We have previously shown cross-linking alpha 4 beta 1 5 fibronectin receptors on human NK cells stimulates phosphorylation two proteins migrating at 105 115 kDa. Here we report integrins PTK activity PYK-2. PYK-2 was maximal min...