A5074228388- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Wnt/β-catenin signaling in development and cancer
- Skin and Cellular Biology Research
- Renal and related cancers
- Cancer Genomics and Diagnostics
- Pluripotent Stem Cells Research
- Erythrocyte Function and Pathophysiology
- Congenital heart defects research
- Monoclonal and Polyclonal Antibodies Research
- Hippo pathway signaling and YAP/TAZ
- Cancer-related gene regulation
- Microbial infections and disease research
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Signaling Pathways in Disease
- Protease and Inhibitor Mechanisms
- Blood properties and coagulation
- RNA Research and Splicing
- Blood groups and transfusion
- Heat shock proteins research
- Molecular Biology Techniques and Applications
- Educational Reforms and Innovations
- Educational Robotics and Engineering
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
1994-2013
Ospedale Maggiore
2007-2009
Istituti di Ricovero e Cura a Carattere Scientifico
2007-2009
University of Milano-Bicocca
2005-2009
San Raffaele University of Rome
2005
Vita-Salute San Raffaele University
2004
European Molecular Biology Laboratory
2003
New York University
1993-2000
Centre National de la Recherche Scientifique
1991
The transcription factor Sox2 is active in neural stem cells, and Sox2`knockdown' mice show defects stem/progenitor cells the hippocampus eye, possibly some neurons. In humans, heterozygous deficiency associated with eye abnormalities, hippocampal malformation epilepsy. To better understand role of Sox2, we performed vitro differentiation studies on cultured from embryonic adult brains `knockdown' mutants. expression high undifferentiated declines differentiation, but remains visible at...
To examine the function of alpha 6 beta 4 integrin we have determined its ligand-binding ability and overexpressed two potentially dominant negative mutant subunits, lacking either cytoplasmic or extracellular domain, in bladder epithelial 804G cells. The results cell adhesion radioligand-binding assays showed that is a receptor for several laminin isoforms, including 1, 2, 4, 5. Overexpression tail-less head-less subunit did not suppress 4-mediated to laminins, as both types transfectants...
The alpha 6 beta 4 integrin is structurally distinct from all the other known integrins because cytoplasmic domain of unusually large and contains four type III fibronectin-like modules toward its C-terminus. To examine function tail, we have expressed full-length truncated human cDNAs in rat bladder epithelial 804G cells, which form hemidesmosome-like adhesions vitro. cDNA encoded wild-type subunit associated with endogenous was recruited at cell surface within adhesions. A recombinant 4,...
The integrin β4 has a long cytodomain necessary for hemidesmosome formation. A yeast two-hybrid screen using uncovered protein called p27BBP that represents interactor. Both in and vitro, binds the two NH2-terminal fibronectin type III modules of β4, region required signaling Sequence analysis revealed was not previously known no homology with any isolated mammalian protein, but 85% identical to gene product unknown function. Expression studies by Northern situ hybridization showed that,...
Cadherin-mediated cell-cell adhesion is dynamically modulated during epithelial-mesenchymal transition triggered by activation of receptor tyrosine kinases (RTK) in epithelial cells. Several cadherin-binding proteins have been identified that control adhesion. However, the mechanisms which intercellular and cell motility are coregulated still unknown. Here, we delineate a hitherto uncharted cooperation between RTKs, RhoA GTPase, p120 catenin instructing motile behavior to We found expression...
Experimental data suggest that cell-based therapies may be useful for cardiac regeneration following ischaemic heart disease. Bone marrow (BM) cells have been reported to contribute tissue repair after myocardial infarction (MI) by a variety of humoural and cellular mechanisms. However, there is no direct evidence, so far, BM can generate stem (CSCs). To investigate whether repopulate the Kit(+) CSCs pool, we transplanted from transgenic mice, expressing green fluorescent protein under...
Here, we show that the subcellular localization of alpha-like RNA polymerase II core subunit 3 (RPB3) is regulated during muscle differentiation. We have recently demonstrated expression RPB3 differentiation and that, inside (RNAP II), it directly involved in contacting regulatory proteins such as myogenic transcription factor Myogenin activating ATF4. for first time, RPB3, addition to its presence role RNAP enzyme, accumulates cytoplasm cycling cells migrates nucleus upon induction program....
Background During late differentiation, erythroid cells undergo profound changes involving actin filament remodeling. One of the proteins controlling dynamics is gelsolin, a calcium-activated severing and capping protein. Gelsolin-null (Gsn−/−) mice generated in C57BL/6 background are viable fertile.1Design Methods We analyzed functional roles gelsolin erythropoiesis by: (i) evaluating expression murine fetal liver at different stages differentiation (using reverse transcription polymerase...
SUMMARY: BACKGROUND: In a previous publication we described method for Jk(a)/Jk(b), Fy(a)/Fy(b), S/s, K/k, Kp(a)/Kp(b), Js(a)/Js(b), Co(a)/Co(b), and Lu(a)/Lu(b) genotyping based on microsphere suspension array. Here, an improved version of the assay is presented. METHODS: TWO MULTIPLEX POLYMERASE CHAIN REACTIONS (PCR) WERE DEVELOPED: one amplification samples routinely tested other those systems that are less frequently. Each biotinylated PCR product hybridized in single multiplex assay. A...