A5015227791- RNA Research and Splicing
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Neurogenesis and neuroplasticity mechanisms
- Tissue Engineering and Regenerative Medicine
- Fuel Cells and Related Materials
- RNA and protein synthesis mechanisms
- Cancer-related Molecular Pathways
- Mesenchymal stem cell research
- Cancer Genomics and Diagnostics
- Nerve injury and regeneration
- MicroRNA in disease regulation
- Wnt/β-catenin signaling in development and cancer
- Advanced Glycation End Products research
- Animal Genetics and Reproduction
- RNA Interference and Gene Delivery
- Cutaneous Melanoma Detection and Management
- interferon and immune responses
- Erythropoietin and Anemia Treatment
- Congenital heart defects research
- Electrospun Nanofibers in Biomedical Applications
- Virus-based gene therapy research
University of Milan
2014-2019
University of Trento
2012-2016
Fondazione IRCCS Istituto Neurologico Carlo Besta
2015
University of Modena and Reggio Emilia
2012
University of Milano-Bicocca
2008-2009
San Raffaele University of Rome
2002
The transcription factor Sox2 is active in neural stem cells, and Sox2`knockdown' mice show defects stem/progenitor cells the hippocampus eye, possibly some neurons. In humans, heterozygous deficiency associated with eye abnormalities, hippocampal malformation epilepsy. To better understand role of Sox2, we performed vitro differentiation studies on cultured from embryonic adult brains `knockdown' mutants. expression high undifferentiated declines differentiation, but remains visible at...
Epithelial-to-mesenchymal transition (EMT) is a core process underlying cell movement during embryonic development and morphogenesis. Cancer cells hijack this developmental program to execute multi-step cascade, leading tumorigenesis metastasis. CD133 (PROM1), marker of cancer stem cells, has been shown facilitate EMT in various cancers, but the regulatory networks controlling gene expression function remain incompletely delineated. In study, we show that ribonucleoprotein complex including...
Abstract Post-transcriptional regulation is an essential determinant of gene expression programs in physiological and pathological conditions. HuR a RNA-binding protein that orchestrates the stabilization translation mRNAs, critical inflammation tumor progression, including necrosis factor-alpha (TNF). We identified low molecular weight compound 15,16-dihydrotanshinone-I (DHTS), well known traditional Chinese medicine practice, through validated high throughput screening on set...
Abstract Background HuR, an RNA binding protein involved in the post-transcriptional regulation of a wide spectrum mRNAs, has been demonstrated to be determinant carcinogenesis and tumor aggressiveness several cancer types. In this study, we investigated role HuR apoptosis chemoresistance induced by widely used anticancer drug doxorubicin human breast cells (MCF-7). Results We showed that acts early phase cell response doxorubicin, being translocate into cytoplasm upon phosphorylation....
The subcutaneous adipose tissue provides a clear advantage over other mesenchymal stem cell sources due to the ease with which it can be accessed, as well of isolating residing cells. Human adipose-derived cells (hADSCs), localized in stromal–vascular portion, isolated ex vivo using combination washing steps and enzymatic digestion. In this study, we report that microfragmented human lipoaspirated is better source compared normal tissue. structural composition microfragments comparable...
LncRNAs play crucial roles in cellular processes and their regulatory effects the adult brain neural stem cells (NSCs) remain to be entirely characterized. We report that 10 lncRNAs (LincENC1, FABL, lincp21, HAUNT, PERIL, lincBRN1a, lincBRN1b, HOTTIP, TUG1 FENDRR) are expressed during murine NSCs differentiation interact with RNA-binding protein ELAVL1/HuR. Furthermore, we characterize function of two deregulated lncRNAs, lincBRN1a NSCs' differentiation. Their inhibition leads induction...
The transcription factor Sox2 is essential for neural stem cells (NSC) maintenance in the hippocampus and vitro . Emx2 also critical hippocampal development NSC self-renewal. Searching 'modifier' genes affecting deficiency phenotype mouse, we observed that loss of one allele substantially increased telencephalic β-geo (LacZ) expression a transgene driven by 5′ or 3′ enhancer. Reciprocally, overexpression cultures inhibited activity same transgene. In vivo , levels medial wall, including...
// Alessandra Bisio 1 , Elisa Latorre 2 Virginia Andreotti 3 Brigitte Bressac-de Paillerets 4 Mark Harland 5 Giovanna Bianchi Scarra Paola Ghiorzo Robert C. Spitale 6 Alessandro Provenzani Alberto Inga Laboratory of Transcriptional Networks, Centre for Integrative Biology, CIBIO, University Trento, Italy Genomic Screening, Genetics Rare Hereditary Cancers, DiMI, Genoa, and IRCCS AOU San Martino-IST, Service de Génétique, Institut Gustave Roussy, Villejuif, France Section Epidemiology...
The protein kinase C<i>δ</i> (PKC<i>δ</i>) interacts with and phosphorylates HuR, dictating its functionality. We show here that the genotoxic stimulus induced by doxorubicin triggers PKC<i>δ</i> interaction HuR leads to phosphorylation on serines 221 318 cytoplasmic translocation. This series of events is crucial elicit death pathway triggered necessary promote function in post-transcriptional regulation gene expression, because genetic ablation caused inability bind target mRNAs,...
// Natthakan Thongon 1, * , Ilaria Castiglioni 2, Chiara Zucal 1 Elisa Latorre Vito D'Agostino Inga Bauer 3 Michael Pancher 4 Alberto Ballestrero Georg Feldmann 5 Alessio Nencioni Alessandro Provenzani Laboratory of Genomic Screening, Centre for Integrative Biology, University Trento, Italy 2 Gene Expression and Muscular Dystrophy, San Raffaele Scientific Institute, Milan, Department Internal Medicine, Genoa, High Throughput Screening Facility, Pancreatic Cancer Translational Research,...
Erythropoietin-releasing neural precursor cells (Er-NPCs) are a subclass of subventricular zone-derived progenitors, capable surviving for 6 hr after death donor. They present higher differentiation. Here, Er-NPCs were studied in animal model Parkinson’s disease. Dopaminergic degeneration was caused by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine intraperitoneal administration C57BL/6 mice. The loss function evaluated specific behavioral tests. (2.5 × 10 5 ) expressing the green fluorescent...
Since 2005, sex determining region y–box 2 (SOX2) has drawn the attention of scientific community for being one key transcription factors responsible pluripotency induction in somatic stem cells. Our research investigated turnover regulation SOX2 mRNA human adipose-derived cells, considered most valuable sources cells regenerative medicine. Mitoxantrone is a drug that acts on nucleic acids primarily used to treat certain types cancer and was recently shown ameliorate outcome autoimmune...
Abstract In mammalian systems, controlled progression through the cell cycle is essential for normal proliferation and its loss a hallmark of malignancy. p16INK4a inhibits cyclin-dependent kinases CDK4 CDK6, thereby keeping retinoblastoma protein (pRB) in hypo-phosphorylated state, which can lead to G1/S checkpoint activation. addition plays crucial role process replicative senescence. or inactivation associated with predisposition melanoma, pancreatic cancer other malignancies, highlighting...
Abstract CDKN2A/p16INK4a is an important tumor-suppressor gene whose dysregulation associated with melanoma. We have recently demonstrated that the p16INK4a expression can be modulated by IRES-dependent mRNA translation and this regulation might role during tumorigenesis. also identified YBX1 as RNA binding protein targeting stimulating p16 efficiency. The IRES function was particularly active in hypoxia inhibition of mTOR. This post-transcriptional regulatory mechanism would potentially a...
<div>Abstract<p>Epithelial-to-mesenchymal transition (EMT) is a core process underlying cell movement during embryonic development and morphogenesis. Cancer cells hijack this developmental program to execute multi-step cascade, leading tumorigenesis metastasis. CD133 (PROM1), marker of cancer stem cells, has been shown facilitate EMT in various cancers, but the regulatory networks controlling gene expression function remain incompletely delineated. In study, we show that...
<p>Quality controls on HuR and MALAT1 silencing</p>