A5008779360- Muscle Physiology and Disorders
- Cancer-related Molecular Pathways
- RNA Research and Splicing
- Histone Deacetylase Inhibitors Research
- MicroRNA in disease regulation
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- Cell death mechanisms and regulation
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Anesthesia and Pain Management
- Circular RNAs in diseases
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Fungal and yeast genetics research
- Signaling Pathways in Disease
- NF-κB Signaling Pathways
- Endoplasmic Reticulum Stress and Disease
- Ion channel regulation and function
- Toxin Mechanisms and Immunotoxins
- Heat shock proteins research
- Systemic Sclerosis and Related Diseases
- Cancer, Stress, Anesthesia, and Immune Response
University of L'Aquila
2006-2024
Chinese Academy of Fishery Sciences
2017
National Institute of Arthritis and Musculoskeletal and Skin Diseases
2004-2007
National Institutes of Health
2004-2007
QIMR Berghofer Medical Research Institute
2007
Labor (Italy)
2007
Deutsches Historisches Institut Rom
2005
University of Wisconsin–Madison
2004
Cancer Institute (WIA)
2003
The Ezh2 protein endows the Polycomb PRC2 and PRC3 complexes with histone lysine methyltransferase (HKMT) activity that is associated transcriptional repression. We report expression was developmentally regulated in myotome compartment of mouse somites its down-regulation coincided activation muscle gene differentiation satellite-cell-derived myoblasts. Increased inhibited differentiation, this property conferred by SET domain, required for HKMT activity. In undifferentiated myoblasts,...
The mechanism of skeletal myoblast fusion is not well understood. We show that endogenous nitric oxide (NO) generation required for both in embryonic myoblasts and satellite cells. effect NO concentration time dependent, being evident only at the onset differentiation, direct on process itself. action mediated through a tightly regulated activation guanylate cyclase cyclic guanosine monophosphate (cGMP), so much deregulation cGMP signaling leads to fusion-induced hypertrophy...
Summary Systemic sclerosis (SSc) is a chronic disease, with early activation of the immune system. The aim our work was to address how SSc–mesenchymal stem cells (MSCs), although senescent, might preserve specific immunomodulatory abilities during SSc. MSCs were obtained from 10 SSc patients and healthy controls (HC). Senescence evaluated by assessing cell cycle, β-galactosidase (β-Gal) activity, p21 p53 expression; doxorubicin used as acute senescence stimulus evaluate their ability react...
hRPB11 is a core subunit of RNA polymerase II (pol II) specifically down-regulated on doxorubicin (dox) treatment. Levels this protein profoundly affect cell differentiation, proliferation, and tumorigenicity in vivo. Here we describe Che-1, novel human that interacts with hRPB11. Che-1 possesses domain high homology Escherichia coli final sigma-factor 70 SV40 large T antigen. In addition, report the retinoblastoma susceptibility gene (Rb) by two distinct domains. Functionally, demonstrate...
MyoD is sufficient to initiate the skeletal muscle gene expression program. Transcription of certain target genes occurs in early phases, whereas that others induced only at later stages, although present throughout differentiation process. acetylation regulates transcriptional competency, yet whether this post-translational modification equally relevant for activation all targets unknown. Moreover, molecular mechanisms through which ensures achieves its optimal activity remain unexplored....
We have previously demonstrated that DNA damage leads to stabilization and accumulation of Che-1, an RNA polymerase II-binding protein plays important role in transcriptional activation p53 maintenance the G(2)/M checkpoint. Here we show Che-1 is down-regulated during apoptotic process. found E3 ligase HMD2 physically functionally interacts with promotes its degradation via ubiquitin-dependent proteasomal system. Furthermore, response stimuli peptidyl-prolyl isomerase Pin1 conformational...
Che-1 is a recently identified human RNA polymerase II binding protein involved in the regulation of gene transcription and cell proliferation. We previously demonstrated that inhibits Rb growth-suppressing function by interfering with Rb-mediated HDAC1 recruitment on E2F target promoters. By hybridization cancer profile arrays, we found expression strongly down-regulated several tumors, including colon kidney carcinomas, compared relative normal tissues. Consistent these data,...
Pediatric neuroblastomas (NBs) are heterogeneous, aggressive, therapy-resistant embryonal tumors that originate from cells of neural crest origin committed to the sympathoadrenal progenitor cell lineage. Stress- and drug-resistance mechanisms drive post-therapeutic relapse metastatic progression, characterization inhibition which major goals in improving therapeutic responses. NBs include alternative TrkAIII splicing neurotrophin receptor tropomyosin-related kinase A (NTRK1/TrkA), correlates...
RPB3 is a core subunit of RNA polymerase II (pol II) that, together with the RPB11 subunit, forms heterodimer considered as functional counterpart bacterial α homodimer involved in promoter recognition. We previously employed yeast two‐hybrid system and identified an interaction between myogenic transcription factor myogenin, demonstrating involvement this muscle differentiation. In paper we report another known factor, ATF4. found that intensity ATF4 similar to one myogenin. This involves...
RNA polymerase II core subunit 3 (RPB3) is an a-like of (pol II). It selectively down-regulated upon treatment with doxorubicin (dox). Due to the failure skeletal muscle cells differentiate when exposed dox, we hypothesized that RPB3 involved in differentiation. To this end, have isolated human RPB3-interacting proteins by using yeast two-hybrid screening. interest interaction between and myogenic transcription factor myogenin was identified. This involves a specific region protein not...
Here, we show that the subcellular localization of alpha-like RNA polymerase II core subunit 3 (RPB3) is regulated during muscle differentiation. We have recently demonstrated expression RPB3 differentiation and that, inside (RNAP II), it directly involved in contacting regulatory proteins such as myogenic transcription factor Myogenin activating ATF4. for first time, RPB3, addition to its presence role RNAP enzyme, accumulates cytoplasm cycling cells migrates nucleus upon induction program....
We previously isolated the human RPB11 cDNA, encoding 13.3 kDa subunit of RNA polymerase II, and demonstrated that expression this is modulated by doxorubicin. Using hRPB11 as bait in a yeast two-hybrid system, two cDNA variants second II subunit, hRPB3, have now been characterized. These hRPB3 mRNA species differed 3' UTR region length, longer transcript containing AU-rich sequence motif mediates degradation. Both transcripts share similar pattern distribution adult tissues, with...
Aldolase is a key enzyme involved in glycolysis, gluconeogenesis, and the pentose phosphate pathway. To establish expression patterns of all three aldolase isozyme genes different tissues during early embryogenesis lower vertebrates, as well to explore functional differences between these isozymes, grass carp was selected model owing its relatively high glucose-metabolizing capability. Based on cDNA sequences A, B, C genes, isozymes were analyzed using quantitative real-time polymerase chain...