A5044399312- Epigenetics and DNA Methylation
- Bone and Dental Protein Studies
- Cancer Research and Treatments
- Cell death mechanisms and regulation
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- S100 Proteins and Annexins
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Prostate Cancer Treatment and Research
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- Cancer, Lipids, and Metabolism
- Cancer-related gene regulation
- Mast cells and histamine
- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- ATP Synthase and ATPases Research
- Vitamin D Research Studies
- Click Chemistry and Applications
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Estrogen and related hormone effects
Fondazione IRCCS Istituto Nazionale dei Tumori
2016-2025
University of Rome Tor Vergata
2024
Tumori Foundation
2022
University of Milan
2009-2015
XIAP (X-linked inhibitor of apoptosis protein) is an anti-apoptotic protein exerting its activity by binding and suppressing caspases. As overexpressed in several tumours, which it apparently contributes to chemoresistance, because vivo antagonised second mitochondria-derived activator caspase (SMAC)/direct apoptosis-binding with low pI, small molecules mimicking SMAC (so called SMAC-mimetics) can potentially overcome tumour resistance promoting apoptosis. Three homodimeric compounds were...
Abstract Tumor growth and development is determined by both cancer cell–autonomous microenvironmental mechanisms, including the contribution of infiltrating immune cells. Because role mast cells (MC) in this process poorly characterized even controversial, we investigated their part breast cancer. Crossing C57BL/6 MMTV-PyMT mice, which spontaneously develop mammary carcinomas, with MC-deficient C57BL/6-KitW-sh/W-sh (Wsh) showed that MCs promote tumor prevent basal CK5-positive areas favor a...
Progression to stage IV disease remains the main cause of breast cancer-related deaths. Increasing knowledge on hematogenous phase metastasis is key for exploiting entire window opportunity interfere with early dissemination and achieve a more effective control. Recent evidence suggests that circulating tumor cells (CTCs) possess diverse adaptive mechanisms survive in blood eventually metastasize, encouraging research into CTC-directed therapies.On hypothesis distinguishing molecular...
Highlights•ZEB1+ AML blasts are immune regulatory via Th17 expansion•IL-17 promotes proliferation and invasion of ZEB1+ cells•IL-17 ZEB1 binding to target gene promoters•ZEB1 blast features combine regulation chemoresistanceSummaryAcute myeloid leukemia (AML) progression is influenced by suppression induced cells. ZEB1, a critical transcription factor in epithelial-to-mesenchymal transition, demonstrates functions AML. Silencing leukemic cells reduces engraftment extramedullary disease...
Smac mimetics (SMs) comprise a class of small molecules that target members the inhibitor apoptosis family pro-survival proteins, whose expression in cancer cells hinders action conventional chemotherapeutics. Herein, we describe activity SM83, newly synthesised dimeric SM, two ascites models: athymic nude mice injected intraperitoneally with IGROV-1 human ovarian carcinoma and immunocompetent BALB/c murine Meth A sarcoma cells. SM83 rapidly killed ascitic vivo (prolonging mouse survival),...
Chk2 is a nuclear protein kinase involved in the DNA damage-induced ataxia telangiectasia mutated-dependent checkpoint arrest at multiple cell cycle phases. Searching for Chk2-binding proteins by yeast two-hybrid system, we identified strong interaction with karyopherin-α2 (KPNA-2), gene product active import of bearing localization signal (NLS). This finding was confirmed glutathione S-transferase pull-down and co-immunoprecipitation assays. Of three predicted NLSs, located amino acids...
Breast cancer is the most common type of in women worldwide, with luminal subtype being widespread. Although characterized by better prognosis compared other subtypes, breast still considered a threatening disease due to therapy resistance, which occurs via both cell- and non-cell-autonomous mechanisms. Jumonji domain-containing 6, arginine demethylase lysine hydroxylase (JMJD6) endowed negative prognostic value and, its epigenetic activity, it known regulate many intrinsic cell pathways. So...
Artemis plays a crucial role in the hairpin‐opening step of antigen receptor VDJ gene recombination presence catalytic subunit deoxyribonucleic acid (DNA)‐dependent protein kinase (DNA‐PKcs). A defect causes human radiosensitive‐severe combined immunodeficiency. Cells from Artemis‐deficient patients and mice display increased chromosomal instability, but precise function this factor response to DNA damage remains be elucidate. In study, we show that is hyperphosphorylated an...
AbstractREGγ is a member of the 11S regulatory particle that activates 20S proteasome. Studies in REGγ deficient mice indicated an additional role for this protein cell cycle regulation and proliferation control. In paper we demonstrate equally expressed throughout cycle, but undergoes distinctive subcellular localization at mitosis. Thus, while interphase cells nuclear, telophase it localizes on chromosomes, suggesting mitotic progression. Furthermore, found overexpression weakens arrest...
Abstract Loss of ataxia telangiectasia mutated (ATM) kinase, a key factor the DNA damage response (DDR) pathway, causes cancer predisposing and neurodegenerative syndrome ataxia-telangiectasia (A-T). To investigate mechanisms neurodegeneration, we have reprogrammed fibroblasts from ATM-null A-T patients normal controls to pluripotency (human-induced pluripotent stem cells), derived these neural precursor cells able terminally differentiate into post-mitotic neurons positive >90% for β...
Fibroblasts are crucial mediators of tumor-stroma cross-talk through synthesis and remodeling the extracellular matrix production multiple soluble factors. Nonetheless, little is still known about specific determinants fibroblast pro-tumorigenic activity in lung cancer. Here, we aimed at understanding role miRNAs, which often altered stromal cells, reprogramming fibroblasts towards a tumor-supporting phenotype. We employed co-culture-based high-throughput screening to identify miRNAs...