A5049231418- Peptidase Inhibition and Analysis
- Chronic Myeloid Leukemia Treatments
- Cancer Cells and Metastasis
- CAR-T cell therapy research
- Cancer Genomics and Diagnostics
- GDF15 and Related Biomarkers
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Cancer Immunotherapy and Biomarkers
- Cancer-related molecular mechanisms research
- Glioma Diagnosis and Treatment
- Immune cells in cancer
- Ferroptosis and cancer prognosis
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Breast Cancer Treatment Studies
- Neonatal Respiratory Health Research
- SARS-CoV-2 and COVID-19 Research
- Molecular Biology Techniques and Applications
- Circular RNAs in diseases
- Hematopoietic Stem Cell Transplantation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Prostate Cancer Treatment and Research
- Immunotherapy and Immune Responses
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- PI3K/AKT/mTOR signaling in cancer
Fondazione IRCCS Istituto Nazionale dei Tumori
2016-2025
Tumori Foundation
2022
Animal experimentation is a vast ecosystem that tries to make different issues such as legislative, ethical and scientific coexist. Research in animal has made many strides thanks the 3Rs principle attached legislative decrees, but for this very reason, it needs be evenly implemented both among countries have adhered decrees team members who design execute experimental practice. In article, we emphasize importance of principle's application, with particular focus on concept Reduction related...
Progression to stage IV disease remains the main cause of breast cancer-related deaths. Increasing knowledge on hematogenous phase metastasis is key for exploiting entire window opportunity interfere with early dissemination and achieve a more effective control. Recent evidence suggests that circulating tumor cells (CTCs) possess diverse adaptive mechanisms survive in blood eventually metastasize, encouraging research into CTC-directed therapies.On hypothesis distinguishing molecular...
Well-differentiated/dedifferentiated liposarcomas (WD/DDLPSs) account for ∼60% of all liposarcomas. They have a poor prognosis due to limited therapeutic options. WD/DDLPSs are characterized by aberrant expression mouse double minute 2 (MDM2), which forms G-quadruplexes (G4s) in its promoter. Here, we investigated the possibility targeting with small molecules against MDM2 G4s. Among tested, naphthalene diimide derivative QN-302 significantly impaired WD/DDLPS cell growth and activity...
Colorectal cancer (CRC) screening programs help diagnose precursors and early cancers reduce CRC mortality. However, currently recommended tests, the fecal immunochemical test (FIT) colonoscopy, have low uptake. There is therefore a pressing need for strategies that are minimally invasive consequently more acceptable to patients, most likely blood based, increase identification. MicroRNAs (miRNAs) released from cells detectable in plasma remarkably stable form, making them ideal biomarkers....
Predictive biomarkers of response to immune-checkpoint inhibitors (ICIs) are an urgent clinical need. The aim this study is identify manageable parameters use in practice select patients with higher probability ICIs. Two-hundred-and-seventy-one consecutive metastatic solid tumor patients, treated from 2013 until 2017 anti- Programmed death-ligand 1 (PD-L1)/programmed cell death protein (PD-1) ICIs, were evaluated for baseline lactate dehydrogenase (LDH) serum level, performance status (PS),...
Neutrophil extracellular traps (NETs) are web-like chromatin structures composed by dsDNA and histones, decorated with antimicrobial proteins. Their interaction dendritic cells (DCs) allows DC activation maturation toward presentation of NET-associated antigens. Differently from other types cell death that imply protein denaturation, NETosis preserves the proteins localized onto DNA threads for proper enzymatic activity conformational status, including immunogenic epitopes. Besides...
Cancer is a systemic disease able to reprogram the bone marrow (BM) niche towards protumorigenic state. The impact of cancer on specific BM subpopulations can qualitatively differ according signals released by tumor, which vary basis tissue origin. Using spontaneous model mammary carcinoma, we identified mesenchymal stem cells (MSC) as first sensors distal and key mediators reprogramming. Through release IL1B, MSCs induced transcriptional upregulation nuclear translocation activating...
Abstract Purpose: Epithelioid hemangioendothelioma (EHE), an ultra-rare sarcoma, poses therapeutic challenges because of limited efficacy conventional chemotherapy in advanced cases, necessitating exploration new treatment avenues and identification novel aggressive biomarkers. This study aimed at (i) utilizing a patient-derived xenograft model EHE its associated cell line to assess the sirolimus (ii) analyzing two distinct patient cohorts pinpoint circulating biomarkers aggressiveness....
In population-based screens, tissue biopsy remains the standard practice for women with imaging that suggests breast cancer. We examined circulating microRNAs as minimally invasive diagnostic biomarkers to discriminate malignant from benign lesions. miRNAs were analyzed by OpenArray in a retrospective cohort of plasma samples including 100 patients (T), 89 disease (B), and 99 healthy donors (HD) divided into training testing sets prospective (BABE) 289 suspicious findings who underwent...
Due to the rarity of vaginal intraepithelial neoplasia (VAIN), it is impossible define best treatment approach or assess morbidity. However, brachytherapy (BT) could be a valuable choice for VAIN grade 3 (VAIN3). The aim this paper was report single-institution study application high-dose-rate BT and evaluate clinical outcomes as well investigate dose-effect relationship stenosis.We retrospectively collected hospital records plans 14 consecutive women treated in our department from August...
Active surveillance (AS) has evolved as a strategy alternative to radical treatments for very low risk and low-risk prostate cancer (PCa). However, current criteria selecting AS patients are still suboptimal. Here, we performed an unprecedented analysis of the circulating miRNome investigate whether specific miRNAs associated with disease reclassification can provide refinement standard clinicopathological features improving patient selection. The global miRNA expression profiles were...
Prostate cancer (PCa) ranges from indolent to aggressive tumors that may rapidly progress and metastasize. The switch PCa is fostered by reactive stroma infiltrating tumor foci. Therefore, stroma-based biomarkers potentially improve the early detection of PCa, ameliorating disease classification. Gene expression profiles fibroblasts highlighted up-regulation genes related deposition, including periostin sparc. Here, potential as a stromal biomarker has been investigated on prostatectomies...
Breast cancer (BC) is a heterogeneous disease in which the tumor microenvironment (TME) seems to impact clinical outcome. Here, we investigated whether combination of gene expression signatures relating both structural and immune TME aspects can help predict prognosis women with high-grade BC (HGBC). Thus, focused on combined molecular biomarker variable that involved extracellular matrix (ECM)-associated (ECM3 signature) interferon (IFN)-associated metagene (IFN metagene) expression. In 97...
Purpose To study a robust and reproducible procedure to investigate relation between focal brain radiotherapy (RT) low doses, neurocognitive impairment late White Matter Gray alterations, as shown by Diffusion Tensor Imaging (DTI), in children. Methods materials Forty-five patients (23 males 22 females, median age at RT 6.2 years, evaluations 11.1 years) who had received for tumors were recruited DTI exams tests. Patients’ brains parceled 116 regions of interest (ROIs) using an available...
Abstract BACKGROUND Young patients with malignant brain tumors show neurocognitive alterations as both consequences of the tumor and treatments received. A prospective study longitudinal radiation damages after focal RT was activated in 2017. We present first analysis correlating localization, symptoms, neurological/endocrinological impairments, surgery/ies if adopted, cognitive assessments at time enrollment. METHODS Sixty-six children eligible for were enrolled underwent a assessment based...
Abstract Background. Lung cancer (LC) patients, despite innovative treatments such as target and immune-therapies, still have the poorest five-year survival rate. In this context possibility of an early detection can anticipate therapeutic intervention, avoiding aggressive therapies thus prolonging disease-free overall survival. Therefore, identification new circulating biomarkers represents a relevant clinical need. Methods. patients (n=63) heavy smokers (HS) free-subjects (n=52) were...
<div>AbstractPurpose:<p>Epithelioid hemangioendothelioma (EHE), an ultra-rare sarcoma, poses therapeutic challenges because of limited efficacy conventional chemotherapy in advanced cases, necessitating exploration new treatment avenues and identification novel aggressive biomarkers. This study aimed at (i) utilizing a patient-derived xenograft model EHE its associated cell line to assess the sirolimus (ii) analyzing two distinct patient cohorts pinpoint circulating biomarkers...
<p>Sirolimus inhibited the growth of patient-derived models EHE. <b>A,</b> Cell inhibition curves obtained after exposure to different doses doxorubicin or sirolimus. Data are reported as percentage drug-treated cells compared with control and represent mean ± SD three independent experiments. <b>B,</b> Growth reporting RTW (mean SEM) in doxorubicin- sirolimus-treated mouse groups (nine mice/group), which 1 indicates tumor weight at beginning treatment. The...
<p>Supplementary Figure 4. Transcriptomic profiles of EHE clinical tumor and PDX.</p>
<p>Characterization of EHE PDX and paired cell line. Representative pictures the clinical sample corresponding model. <b>A,</b> The histology was assessed on hematoxylin eosin–stained slides. Scale bar, 100 µm. <i>WWTR1::CAMTA1</i> dual-color dual-fusion FISH pattern in diploid tumor cells showing one single fusion, consistently with genomic profile. <b>B,</b> Genomic profile (left) (right). <b>C,</b> Loss (blue) gain (red) were depicted...
<p>Supplementary Figure 10. GDF-15 down-regulation induced by sirolimus and/or ATF4/5 silencing.</p>
<p>Supplementary Figure 3. Chromosomal architecture of the PDX compared to clinical sample in chromosomes 7 and 11.</p>
<p>Circulating GDF-15 levels correlated with disease aggressiveness in patients EHE. <b>A,</b> Analysis of circulating cytokines plasma samples 16 EHE and four healthy donors using the Human XL Cytokine Array showed higher compared donors. <b>B,</b> levels, as detected by ELISA expressed pg/mL plasma, a training (retrospective; 8 higher-risk 12 lower-risk 32 donors) testing (prospective; 6 15 cohort both cohorts. <b>C,</b> Two clinical cases are...