A5049251176- Breast Cancer Treatment Studies
- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Molecular Biology Techniques and Applications
- Cancer-related Molecular Pathways
- Lung Cancer Treatments and Mutations
- BRCA gene mutations in cancer
- Estrogen and related hormone effects
- Gene expression and cancer classification
- Lymphoma Diagnosis and Treatment
- HER2/EGFR in Cancer Research
- Bioinformatics and Genomic Networks
- Ovarian cancer diagnosis and treatment
- Cancer, Lipids, and Metabolism
- Breast Lesions and Carcinomas
- Head and Neck Cancer Studies
- Cancer Cells and Metastasis
- Drug Transport and Resistance Mechanisms
- Cancer-related molecular mechanisms research
- DNA Repair Mechanisms
- Protease and Inhibitor Mechanisms
- Iron Metabolism and Disorders
- Bone and Dental Protein Studies
- Polyomavirus and related diseases
- Erythropoietin and Anemia Treatment
Fondazione IRCCS Istituto Nazionale dei Tumori
2013-2023
University of Milan
2009-2014
IFOM
2005-2013
ASST Melegnano e della Martesana
1993
Institute of Cell Biology and Neurobiology
1992
Journal Article The Bcl-2 Protein: a Prognostic Indicator Strongly Related to p53 Protein in Lymph Node-Negative Breast Cancer Patients Get access Rosella Silvestrini, Silvestrini Search for other works by this author on: Oxford Academic PubMed Google Scholar Silvia Veneroni, Veneroni Maria Grazia Daidone, Daidone Elvira Benini, Benini Patrizia Boracchi, Boracchi Maura Mezzetti, Mezzetti Giovanni Di Fronzo, Fronzo Franco Rilke, Rilke Umberto Veronesi JNCI: of the National Institute, Volume...
At present, most decisions concerning the use of adjuvant therapy in lymph node-negative breast cancer patients are made on basis traditional factors such as tumor size, nodal status, and histopathologic features. However, prognostic being investigated that could identify high-risk groups better address treatment efforts for those patients. Identification more accurate markers, expression mutant p53 protein encoded by (also known TP53) suppressor gene, reproducible, easily assessable,...
Abstract Background Aberrant expression of microRNAs, small non-coding RNA molecules that post-transcriptionally repress gene expression, seems to be causatively linked the pathogenesis cancer. In this context, miR-21 was found overexpressed in different human cancers (e.g. glioblastoma, breast cancer). addition, it is thought endowed with oncogenic properties due its ability negatively modulate tumor-suppressor genes PTEN ) and cause reversion malignant phenotype when knocked- down several...
BACKGROUND AND PURPOSE The tumor-suppressor gene TP53 and the proto-oncogene bcl-2 encode, respectively, for a nuclear phosphoprotein mitochondrial protein involved in multiple cellular functions. proteins provide prognostic information node-negative breast cancer are supposed to influence treatment responsiveness. We analyzed predictive role of p53 expression, alone association with other variables, postmenopausal women node-positive, estrogen receptor-positive (ER+) cancers treated radical...
Data from microarray studies have been used to develop predictive models for treatment outcome in breast cancer, such as a recently proposed model antiestrogen response after tamoxifen that was based on the expression ratio of two genes. We attempted validate this an independent cohort 58 patients with resectable estrogen receptor-positive cancer. measured genes HOXB13 and IL17BR real time-quantitative polymerase chain reaction assessed association between their by use univariate logistic...
Abstract Background Gene expression profiling is moving from the research setting to practical clinical use. signatures able correctly identify high risk breast cancer patients as well predict response treatment are currently under intense investigation. While technical issues dealing with RNA preparation, choice of array platforms, statistical analytical tools taken into account, tissue collection process seldom considered. The time elapsed between surgical removal and freezing samples for...
Changes in amount and composition of extracellular matrix (ECM) are considered a hallmark tumor development. We tested the hypothesis that abnormal production ECM components leads to blood-released molecules representing circulating biomarkers. Candidate genes were selected through class comparison two publicly available datasets confirmed paired normal associated fibroblasts from breast carcinoma (BC) specimens. Production release evaluated human dermal (NHDFs) treated with conditioned...
•ctDNA was detected in 77% of early-stage TNBC patients undergoing neoadjuvant chemotherapy.•Patients with still detectable ctDNA after NAC were more than twice as likely to relapse those undetectable levels.•Detection during follow-up antedated clinical overt metastases up 13 months.•ctDNA all but one non-recurrent patient a temporary peak only 1 8 samples tested.•CTCs progressing cases lacked epithelial surface markers and showed therapeutically exploitable molecular features. BackgroundAs...
In clinical breast cancer research, the utility of certain biomarkers as predictors response to surgery, chemotherapy, or hormonal therapy has been studied intensively. Much less research done on relevance biologic radiotherapy, which represents an effective local-regional treatment for cancer.The involved in DNA damage repair (p53 protein), control programmed cell death and Bcl-2 proteins), cellular detoxification (glutathione S-transferase-pi [GST-pi] enzyme) predicting local recurrence...
Abstract. The potential of different methods to investigate proliferative activity cell populations was analysed for non‐Hodgkin's lymphomas. Cells in S phase and all cycling cells were determined on suspensions obtained from fresh lymph node material by [ 3 H]‐thymidine autoradiography ([ H]TdR LI), a monoclonal antibody bromodeoxyuridine (BrdU the Ki67. A good correlation observed between values LI BrdU ( r s = 0.90; P < 0.01), 0.62; 0.01) Ki67 0.64; individual Using median approaches...
Background Genome-wide gene expression analyses of tumors are a powerful tool to identify signatures associated with biologically and clinically relevant characteristics for several tumor types under clinical validation by prospective trials. However, handling processing specimens may significantly affect the molecular data obtained from their analysis. We studied effects tissue time on in human normal colon tissues undergoing routine surgical procedures. Methods RNA extracted 15 patients at...
To obtain a more integrated understanding of the different breast cancer phenotypes and to investigate whether bio-molecular profiles can distinguish between specific histotypes, we explored interrelations among several biologic variables indicative of, or related to, hormone dependence, proliferation apoptosis control, angiogenesis in ductal lobular carcinomas, most common histotypes. Oestrogen progesterone receptors, tumour proliferative activity, expression cyclin A, p16ink4A, p27kip1,...
The recent dramatic increase in breast cancer incidence across China with progressive urbanization and economic development has signaled the urgent need for molecular clinical detailing of Chinese population. Our analyses a unique transethnic collection frozen specimens from Shanghai Fudan Cancer Center (Chinese Han) profiled simultaneously an analogous Caucasian Italian series revealed consistent transcriptomic data lacking batch effects. prevalence Luminal A subtype was significantly lower...
We have previously reported hepcidin and ferritin increases in the plasma of breast cancer patients, but not patients with benign disease. hypothesized that these differences systemic iron homeostasis may reflect alterations different iron-related proteins also play a key biochemical regulatory role cancer. Thus, here we explored expression bundle molecules involved both tumorigenesis tissue samples. Enzyme-linked immunosorbent assay (ELISA) or reverse-phase protein array (RPPA), were used...
Hepcidin-25 production is stimulated by systemic inflammation, and it interferes with iron utilization, leading to anemia. This study aimed investigate the relationships between plasma levels of hepcidin, interleukin-6 (IL-6), erythropoietin (EPO) erythroferrone (ERFE) in patients benign breast disease or cancer.Plasma samples from a cohort 131 (47 84 cancer) were subjected evaluation IL-6, EPO ERFE using SELDI-TOF-MS immunoassays.An elevated hepcidin was observed malignant tumors compared...
Interest in translational studies on breast cancer is presently devoted to identify biological predictors of treatment response. In patients with operable cancer, subjected primary and adjuvant chemotherapy, we analyzed the predictivity objective clinical response relapse-free survival markers related different cellular aspects functions. Tumour proliferative rate (evaluated as 3H-thymidine-labelling index, TLI), oestrogen progesterone receptors (ER PgR, evaluated by dextran-coated-charcoal...
Abstract The profiles of functional (proliferative rate and cell distribution in the cycle) phenotypic (nuclear DNA content hormone receptor status) biological markers expression P53 Bcl‐2 proteins were prospectively evaluated breast cancers before after different regimens primary chemotherapy. Overall, changes induced on 2 proliferation indices ( 3 H‐thymidine labelling index, H‐dT LI, flow‐cytometric S‐phase fraction, FCM‐S) mainly consisted a decrease for rapidly proliferating tumours an...
The aim of this study was to define the clinical relevance functional biomarkers, prospectively assessed in a randomized protocol, patients with Stage III-IV epithelial ovarian cancer. protocol compared cisplatin polychemotherapy that included and cyclophosphamide.In subset 168 invasive cancer cell proliferation determined by 3H-thymidine labeling index, DNA ploidy flow cytometry, expression p53, bcl-2, glutathione S-transferase-pi (GST-pi) evaluated immunohistochemistry using antibodies...
Abstract The relation between the [ 3 H]‐thymidine labelling index (3H‐Thy LI), which evaluates S‐phase cells, and monoclonal antibody Ki‐67 (MoAb Ki‐67), recognizes a nuclear antigen expressed during cell cycle, was defined in 35 non‐Hodgkin's lymphomas (NHL). Significantly higher median values of H]‐Thy LI Ki‐67‐positive cells were observed for high‐grade than low‐grade malignancy tumours according to Kiel classification, but with wide overlapping two morphologic subgroups. A significant...